The early detection of cancer cells and their visualization before and after surgery are essential for successful pre-and postoperative disease management. Although fluorescence imaging is a sensitive and versatile tool that is finding increasing use in clinical applications, there is a lack of tumour-targeting fluorophores. We report here a family of fluorescent Schiff base organotin dyes (1: Et 2 NÀ L-SnPh 2 , 2: Et 2 NÀ L-SnBu 2 , 3: MeOÀ L-SnPh 2 , 4: MeOÀ L-SnBu 2 , 5: HOÀ L-SnPh 2 , and 6: HOÀ L-SnBu 2 , where L = 2-hydroxybenzylidene-4-hydroxybenzhydrazine). In addition to one-photon-excited fluorescence, efficient twophoton excitation was demonstrated in compounds 1-4. Two of the compounds (5 and 6), both with hydroxyl substituents, were capable of selective accumulation in HeLa cells, allowing differentiation from normal cells (periodontal ligament cells). Compounds 1 and 3 showed excellent cancer cell staining (HeLa) using two-photon bioimaging, which is promising for biomedicine applications.