Outlook on engineering methylotrophs for one-carbon-based industrial biotechnology

Pham, Diep Ngoc; Nguyen, Anh Duc; Lee, Eun Yeol

doi:10.1016/j.cej.2022.137769

Cited by 8 publications

(10 citation statements)

References 114 publications

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“…Cosubstrate strategy has been widely used for cofactor regeneration in recent years (Chen et al, 2014; Wang et al, 2017). Glucose, sucrose, or glycerol were the most commonly used cosubstrates (Hu et al, 2010; Li, Zhang, et al, 2017; Pham et al, 2022). Specifically, the flux toward oxidative PPP, which mainly generates the cofactor NAD(P)H, was reactivated upon the addition of glucose as the cosubstrate (Wasylenko et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Cosubstrate strategy and microbial electrosynthesis (MES) system to maintain redox balance were used to enhance the production of biofuels and chemicals (Rengasamy et al, 2021; Wang et al, 2017; Zhao & van der Donk, 2003). With the introduction of carbon sources such as glycerol, sucrose, and glucose, cosubstrate strategy activates intracellular glycolysis, PPP, and other catabolism pathways, thereby promoting the synthesis of intracellular reducing cofactors (Hu et al, 2010; Li, Zhang, et al, 2017; Pham et al, 2022). Besides, in MES system, the reduced electron shuttle carries electrons obtained from the cathode into the cell to convert NAD(P) + into NAD(P)H and turns into oxidized electron shuttle.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis

Huang

Chen

Cheng

et al. 2023

Biotech & Bioengineering

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The limited supply of reducing power restricts the efficient utilization of acetate in Yarrowia lipolytica. Here, microbial electrosynthesis (MES) system, enabling direct conversion of inward electrons to NAD(P)H, was used to improve the production of fatty alcohols from acetate based on pathway engineering. First, the conversion efficiency of acetate to acetyl‐CoA was reinforced by heterogenous expression of ackA‐pta genes. Second, a small amount of glucose was used as cosubstrate to activate the pentose phosphate pathway and promote intracellular reducing cofactors synthesis. Third, through the employment of MES system, the final fatty alcohols production of the engineered strain YLFL‐11 reached 83.8 mg/g dry cell weight (DCW), which was 6.17‐fold higher than the initial production of YLFL‐2 in shake flask. Furthermore, these strategies were also applied for the elevation of lupeol and betulinic acid synthesis from acetate in Y. lipolytica, demonstrating that our work provides a practical solution for cofactor supply and the assimilation of inferior carbon sources.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis

Huang

Chen

Cheng

et al. 2023

Biotech & Bioengineering

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“…In this review, we focus on the enzymatic oxidation of methane by aerobic methanotrophs. Recent reviews have addressed methanotroph physiology, engineering, and applications − and the biochemistry, structure, and spectroscopy of MMOs. − ,, Here we address both the biology and chemistry of MMOs, spanning the history of MMO research, while highlighting recent developments and providing an outlook on unresolved questions. Progress toward understanding the molecular complexity of MMOs has required the use of a diverse scientific toolbox, involving methods in biochemistry, molecular biology, computational inorganic chemistry, spectroscopy, and structural biology.…”

Section: Introductionmentioning

confidence: 99%

Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases

Tucci,

Rosenzweig

2024

Chem. Rev.

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Methane is a potent greenhouse gas that contributes significantly to climate change and is primarily regulated in Nature by methanotrophic bacteria, which consume methane gas as their source of energy and carbon, first by oxidizing it to methanol. The direct oxidation of methane to methanol is a chemically difficult transformation, accomplished in methanotrophs by complex methane monooxygenase (MMO) enzyme systems. These enzymes use iron or copper metallocofactors and have been the subject of detailed investigation. While the structure, function, and active site architecture of the copper-dependent particulate methane monooxygenase (pMMO) have been investigated extensively, its putative quaternary interactions, regulation, requisite cofactors, and mechanism remain enigmatic. The iron-dependent soluble methane monooxygenase (sMMO) has been characterized biochemically, structurally, spectroscopically, and, for the most part, mechanistically. Here, we review the history of MMO research, focusing on recent developments and providing an outlook for future directions of the field. Engineered biological catalysis systems and bioinspired synthetic catalysts may continue to emerge along with a deeper understanding of the molecular mechanisms of biological methane oxidation. Harnessing the power of these enzymes will necessitate combined efforts in biochemistry, structural biology, inorganic chemistry, microbiology, computational biology, and engineering.

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“…That is, hydrogen produced from water splitting with renewable energy (e.g., solar energy, wind energy, and tidal energy) [7] is used for the chemical reduction of carbon dioxide to generate organic C1 compounds (e.g., methane, methanol, and formate) [8][9][10] at large industrial scale (Figure 1). The resulting organic C1 compounds can then be efficiently converted into diverse products by engineered C1-utilizing cell factories under mild reaction conditions [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Constructing efficient bacterial cell factories to enable one‐carbon utilization based on quantitative biology: A review

Song,

Feng,

Zhou

et al. 2024

Quant. Biol.

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Developing methylotrophic cell factories that can efficiently catalyze organic one‐carbon (C1) feedstocks derived from electrocatalytic reduction of carbon dioxide into bio‐based chemicals and biofuels is of strategic significance for building a carbon‐neutral, sustainable economic and industrial system. With the rapid advancement of RNA sequencing technology and mass spectrometer analysis, researchers have used these quantitative microbiology methods extensively, especially isotope‐based metabolic flux analysis, to study the metabolic processes initiating from C1 feedstocks in natural C1‐utilizing bacteria and synthetic C1 bacteria. This paper reviews the use of advanced quantitative analysis in recent years to understand the metabolic network and basic principles in the metabolism of natural C1‐utilizing bacteria grown on methane, methanol, or formate. The acquired knowledge serves as a guide to rewire the central methylotrophic metabolism of natural C1‐utilizing bacteria to improve the carbon conversion efficiency, and to engineer non‐C1‐utilizing bacteria into synthetic strains that can use C1 feedstocks as the sole carbon and energy source. These progresses ultimately enhance the design and construction of highly efficient C1‐based cell factories to synthesize diverse high value‐added products. The integration of quantitative biology and synthetic biology will advance the iterative cycle of understand–design–build–testing–learning to enhance C1‐based biomanufacturing in the future.

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Outlook on engineering methylotrophs for one-carbon-based industrial biotechnology

Cited by 8 publications

References 114 publications

Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis

Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis

Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases

Constructing efficient bacterial cell factories to enable one‐carbon utilization based on quantitative biology: A review

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