Outer Surface Protein C Peptide Derived from Borrelia burgdorferi Sensu Stricto as a Target for Serodiagnosis of Early Lyme Disease

Arnaboldi, Paul M.; Seedarnee, Rudra; Sambir, Mariya; Callister, Steven M.; Imparato, Josephine A.; Dattwyler, Raymond J.

doi:10.1128/cvi.00608-12

Cited by 43 publications

(52 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Epitope mapping of B. burgdorferi surface proteins has revealed novel peptides conserved across B. burgdorferi genospecies that are not found in other bacterial species (18). Synthetic antigen peptides derived from these epitopes demonstrate equivalent sensitivity and decreased cross-reactivity (19,20).…”

mentioning

confidence: 99%

Development of a Multiantigen Panel for Improved Detection of Borrelia burgdorferi Infection in Early Lyme Disease

et al. 2015

Self Cite

View full text Add to dashboard Cite

iThe current standard for laboratory diagnosis of Lyme disease in the United States is serologic detection of antibodies against Borrelia burgdorferi. The Centers for Disease Control and Prevention recommends a two-tiered testing algorithm; however, this scheme has limited sensitivity for detecting early Lyme disease. Thus, there is a need to improve diagnostics for Lyme disease at the early stage, when antibiotic treatment is highly efficacious. We examined novel and established antigen markers to develop a multiplex panel that identifies early infection using the combined sensitivity of multiple markers while simultaneously maintaining high specificity by requiring positive results for two markers to designate a positive test. Ten markers were selected from our initial analysis of 62 B. burgdorferi surface proteins and synthetic peptides by assessing binding of IgG and IgM to each in a training set of Lyme disease patient samples and controls. In a validation set, this 10-antigen panel identified a higher proportion of early-Lyme-disease patients as positive at the baseline or posttreatment visit than two-tiered testing (87.5% and 67.5%, respectively; P < 0.05). Equivalent specificities of 100% were observed in 26 healthy controls. Upon further analysis, positivity on the novel 10-antigen panel was associated with longer illness duration and multiple erythema migrans. The improved sensitivity and comparable specificity of our 10-antigen panel compared to two-tiered testing in detecting early B. burgdorferi infection indicates that multiplex analysis, featuring the next generation of markers, could advance diagnostic technology to better aid clinicians in diagnosing and treating early Lyme disease.

show abstract

mentioning

confidence: 99%

Development of a Multiantigen Panel for Improved Detection of Borrelia burgdorferi Infection in Early Lyme Disease

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Due to its ability to stimulate an early immune response, OspC continues to be a protein of interest with respect to LD diagnostics. A novel peptide region of OspC, OspC1, was recently identified, and preliminary data suggest it results in improved performance for the detection of early LD compared with that of pepC10 alone (62.1% versus 49.0%, respectively) (21). Further studies are needed, however, to confirm these findings and to better define a role for these antigens as markers of LD.…”

Section: Performance Characteristics Of the Current Two-tiered Testinmentioning

confidence: 73%

The Past, Present, and (Possible) Future of Serologic Testing for Lyme Disease

Theel

2016

J Clin Microbiol

View full text Add to dashboard Cite

Lyme disease prevails as the most commonly transmitted tick-borne infection in the United States, and serologic evaluation for antibodies to Borrelia burgdorferi remains the recommended modality for diagnosis. This review presents a brief historical perspective on the evolution of serologic assays for Lyme disease and provides a summary of the performance characteristics for the currently recommended two-tiered testing algorithm (TTTA). Additionally, a recently proposed alternative to the traditional TTTA is discussed, and novel methodologies, including immuno-PCR and metabolic profiling for Lyme disease, are outlined.

show abstract

“…All the dogs vaccinated with the vaccine containing OspC became positive as previously described with this vaccine. 1,9,10 6,9-11 protection may not be 100%, which emphasizes that vaccines against B. burgdorferi should not be used as the sole prevention strategy; it is imperative to also use acaricides as well. 12 The B. burgdorferi serological responses in the vaccinated dogs that developed infection were similar to those for dogs in experiment 2 that developed infection without being previously vaccinated.…”

Section: Discussionmentioning

confidence: 99%

“…18 However, care must be taken in attempting indirect comparisons of serological response in different studies, as different derivations of peptides can behave differently. 1,2,16 When the OspF antibody responses in all 36 dogs were compared with those of the C6 peptide, sensitivity and specificity were shown to both be 100%. Because antibodies against the other markers could be induced by either vaccination or infection, the sensitivities and specificities were <100%.…”

Section: Discussionmentioning

confidence: 99%

“…[3][4][5]12 For example, in one study, the seroprevalence rates for dogs residing in Connecticut and Wisconsin were 18.1% (1,846 positive tests from 10,209 samples tested) and 10.2% (6,018 positive tests from 59,070 samples tested), respectively. 4 As B. burgdorferi infection is an important cause of clinical illnesses, such as polyarthritis and nephritis, the ability of veterinarians to make an accurate diagnosis is important.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple antigen target approach using the Accuplex4 BioCD system to detect Borrelia burgdorferi antibodies in experimentally infected and vaccinated dogs

et al. 2015

View full text Add to dashboard Cite

Abstract. The primary objective of our study was to optimize detection of serum antibodies to Borrelia burgdorferi using a new commercial automated fluorescence system (Accuplex4 BioCD system, Antech Diagnostics, Lake Success, New York). The system used multiple natural and artificial peptides-outer surface proteins (OspA, OspC, OspF), an outer membrane protein (P39), and a proprietary synthetic peptide (small Lyme peptide [SLP])-and the results were compared with a commercially available enzyme-linked immunosorbent assay that uses a proprietary peptide (C6). Sera from 4 groups were evaluated: dogs vaccinated with 1 of 3 commercially available vaccines (n = 18); dogs infested with adult Ixodes scapularis (black-legged tick; n = 18); dogs previously vaccinated and then infested with I. scapularis (n = 18); and dogs with B. burgdorferi infection that were then vaccinated (n = 14). All of the vaccines evaluated induced OspA responses. However, antibodies against OspF or C6 were not induced in any of the vaccinated dogs. Additionally, the OspF antibodies had 100% sensitivity and specificity when compared to antibodies against C6 peptide. In B. burgdorferi-infected dogs, antibodies against OspC and SLP were detected in serum sooner than antibodies against the other targets. Low levels of antibodies against OspA developed in 6 of 14 B. burgdorferi-infected, unvaccinated dogs and had the shortest duration compared to the other antibodies. Detection of antibody responses to multiple B. burgdorferi targets with this system can be used to help differentiate vaccinated dogs from exposed dogs as well as acute infection from chronic infection.

show abstract

Outer Surface Protein C Peptide Derived from Borrelia burgdorferi Sensu Stricto as a Target for Serodiagnosis of Early Lyme Disease

Cited by 43 publications

References 37 publications

Development of a Multiantigen Panel for Improved Detection of Borrelia burgdorferi Infection in Early Lyme Disease

Development of a Multiantigen Panel for Improved Detection of Borrelia burgdorferi Infection in Early Lyme Disease

The Past, Present, and (Possible) Future of Serologic Testing for Lyme Disease

Multiple antigen target approach using the Accuplex4 BioCD system to detect Borrelia burgdorferi antibodies in experimentally infected and vaccinated dogs

Contact Info

Product

Resources

About