Outer membrane vesicles produced by <i>Burkholderia cepacia</i> cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

Kim, Se Yeon; Kim, Mi‐Hyun; Son, Joo Hee; Kim, Kyung Sik; Yun, Sung Ho; Kim, Kyeong-Min; Kim, Shukho; Shin, Minsang; Lee, Je Chul

doi:10.1080/21505594.2020.1802193

Cited by 11 publications

(11 citation statements)

References 51 publications

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“…The study revealed a significant cell lysis/death at 12 and 24 hours following in vitro exposure of RTgill-W1 cells to 12.5 μg/mL OMVs. Consistently, Burkholderia cepacia OMVs induced cytotoxicity in host cells treated with ≥10 μg/mL of OMVs for 24h [55]. In addition, OMVs induced cytotoxicity of the salmonid CHSE-214 cells [13].…”

Section: Conservation Of De Srnas In 65 Strains With Variable Degree Of Virulencementioning

confidence: 60%

sRNAs enriched in outer membrane vesicles of pathogenic Flavobacterium psychrophilum interact with immune genes of rainbow trout

Chapagain

Ali

Kidane

et al. 2021

Preprint

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Outer membrane vesicles (OMVs) released by gram-negative bacteria during host-pathogen interactions harbor cargos, such as DNA, RNA, toxins, and virulence factors. We hypothesized that sRNAs carried within OMVs of Flavobacterium psychrophilum interact with host immune genes and affect their expression. OMVs were isolated from F. psychrophilum and visualized using transmission electron microscopy (TEM). RNA-Seq datasets generated from whole-cell F. psychrophilum and their OMVs indicated enrichment of specific sRNAs in the OMVs compared to the parent cell. Fluorescent in situ hybridization (FISH) and confocal microscopy confirmed the expression of a randomly chosen sRNA. Integrated RNA-Seq analyses of host transcriptome and bacterial sRNAs on day 5 post-infection of F. psychrophilum -resistant and -susceptible rainbow trout genetic lines revealed 516 protein-coding, 595 lncRNA, and 116 bacterial sRNA differentially expressed (DE) transcripts. Integrated and network analyses of these DE transcripts revealed immune genes targeted by bacterial sRNAs. On the top of these genes, an isoform encoding anaphase-promoting complex subunit 13 (ANAPC13_1) was highly upregulated and exhibited interaction and reciprocal expression with 21 DE sRNAs enriched in OMVs and/or located in pathogenicity islands (PAIs). In vitro treatment of the rainbow trout epithelial cell line RTgill-W1 with OMVs showed signs of cell autolysis accompanied by dynamic changes in expression of host genes when profiled 24h following treatment. The OMV-enriched sRNAs, soFE013584 and soFE002123, showed high interactions with the protection of telomeres 1 gene (POT1); essential for chromosome stability and cellular viability. Modulation of the host gene expression following OMV-treatment, which favors elements from the phagocytic, endocytic, and antigen presentation pathways in addition to HSP70, HSP90, and cochaperone proteins, provided evidence for a potential role of OMVs in boosting the host immune response. In conclusion, our work identified novel microbial targets and inherent characteristics of OMVs that could open up new avenues of treatment and prevention of fish infections.

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Section: Conservation Of De Srnas In 65 Strains With Variable Degree Of Virulencementioning

confidence: 60%

sRNAs enriched in outer membrane vesicles of pathogenic Flavobacterium psychrophilum interact with immune genes of rainbow trout

Chapagain

Ali

Kidane

et al. 2021

Preprint

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“…Acinetobacter baumannii is becoming epidemic in the hospital. The source of these microorganisms is soil and green grass and they become very aggressive with the overuse of broad-spectrum antibiotics and consequently causing serious wound infections that show drug resistance to various types of antibiotic [27].…”

Section: Discussionmentioning

confidence: 99%

Trauma-related wound infections among patients admitted to emergency teaching hospital in Duhok province, Iraq

Alnakshabandie

Mammani

2023

J Infect Dev Ctries

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Introduction: Wound infection is one of the universal challenges for patients who visit the hospital after trauma and undergo surgery and/or during their admission. Trauma could be due to Road Traffic Accidents (RTA), violence, or Falling from High (FFH). There is tangible evidence of the scope and the danger of hospital-acquired infections, which are far more common and deadly than many people comprehend. Methodology: 280 samples were collected from 140 injured persons, who attended the Emergency Teaching Hospital in Duhok, Iraq from September 2021 to April 2022. 140 samples were collected on the patients’ arrival and 140 samples after admission and treatment. The isolated bacteria were manually diagnosed, and then VITEK®2 compact system was performed for confirmation. Results: 27 microbial species were identified. The common bacterial species detected on patients’ arrival were Staphylococcus epidermidis 22 (19.6%), Escherichia coli 16 (14.3%), Staphylococcus aureus 14 (12.5%), Staphylococcus lentus 10 (8.9%) and Stenotrophomonas maltophilia 6(5.4%). On the 2nd samples, which were collected after patients’ admission, the species were Staphylococcus aureus 35(31.3%), Escherichia coli 13(11.6%), Pseudomonas aeruginosa 12 (10.7%), Staphylococcus epidermidis 10 (8.9%), Acinetobacter baumannii and Klebsiella pneumonia were 8 (7.1%) each. Conclusions: The bacteria that contaminate wounds at the accident time led to serious problems after the admission as they cause wound infection with inappropriate antibiotic administration. It is established that there are differences between the bacterial species detected before and after admission in this study with p = 0.004. Furthermore, it has been demonstrated that some species that are isolated prior to the admission of patients turn hostile afterward.

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“…In addition, due to the nature and sebum oily niche of C. acnes , common proteins involved in lipolysis such as lysophospholipase and triacylglycerol lipase, were identified in the EVs. Although these enzymes are generally considered putative virulence factors, lipases are necessary for C. acnes to maintain healthy skin by metabolizing sebum and releasing fatty acids, which are necessary to keep a proper skin acidity that acts as a natural barrier against harmful pathogens, providing innate skin immunity (Kim et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

New insights into the role ofCutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders

Cros

Mir-Pedrol

Toloza

et al. 2022

Preprint

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Background: Cutibacterium acnes is one of the most prevalent bacteria that form the human skin microbiota and, depending on multifactorial conditions, may act as an opportunistic skin pathogen or can help to maintain the skin homeostasis. Actually, different phylotypes of C. acnes have been associated with different degrees of acne vulgaris development, while others, such as the H1 subtype, have been detected in patients with non-acneic skin. However, due to the physiology of the skin, the skin microbiota neither has direct access to the skin's sebaceous glands nor to the main immune cells, as they are protected by a sebum layer. Therefore, the inter-kingdom communication relies on secreted factors and bacterial extracellular vesicles (EVs). In this context, the purpose of this project is to study the role of EVs secreted by three different phylotypes of C. acnes (A1 as pathogenic, H1 as beneficial and H2 as commensal). Results: Main findings showed that the proteomic profile of the cargo embodied in the EVs reflects unique characteristics of the different C. acnes phylotypes in terms of lifestyle, survival and virulence. Moreover, in vitro skin models showed an extended pro-inflammatory modulation of A1 EVs, while H1 EVs displayed a high sebum-reducing potential. Conclusions: This study has highlighted the role of C. acnes EVs as key modulators during skin alterations, specially H1 EVs as an alternative based-natural treatment to fight acne vulgaris symptomatology.

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Outer membrane vesicles produced by Burkholderia cepacia cultured with subinhibitory concentrations of ceftazidime enhance pro-inflammatory responses

Cited by 11 publications

References 51 publications

sRNAs enriched in outer membrane vesicles of pathogenic Flavobacterium psychrophilum interact with immune genes of rainbow trout

sRNAs enriched in outer membrane vesicles of pathogenic Flavobacterium psychrophilum interact with immune genes of rainbow trout

Trauma-related wound infections among patients admitted to emergency teaching hospital in Duhok province, Iraq

New insights into the role ofCutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders

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