Outer membrane vesicles of Lysobacter sp.

Vasilyeva, Natalia V.; Tsfasman, I. M.; Suzina, N. E.; Stepnaya, O. A.; Kulaev, I. S.

doi:10.1134/s1607672909030041

Cited by 14 publications

(9 citation statements)

References 6 publications

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“…Depending on the species of bacteria, this response can depend on opposite signals. Upregulation of OMV production for Lysobacter occurs in response to low nutrition, whereas for Pseudomonas fragi it occurs in response to the availability of nutrients in meat media (76, 79). Enzymes contained within OMVs might degrade a distant, immobile nutrient, thereby releasing soluble nutrients into the environment.…”

Section: Regulation Of Outer Membrane Vesicle Productionmentioning

confidence: 99%

Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles

Kulp

Kuehn

2010

Annu. Rev. Microbiol.

1,218

1,263

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Gram-negative bacteria produce outer membrane vesicles (OMVs) that contain biologically active proteins and perform diverse biological processes. Unlike other secretion mechanisms, OMVs enable bacteria to secrete insoluble molecules in addition to and in complex with soluble material. OMVs allow enzymes to reach distant targets in a concentrated, protected, and targeted form. OMVs also play roles in bacterial survival: Their production is a bacterial stress response and important for nutrient acquisition, biofilm development, and pathogenesis. Key characteristics of OMV biogenesis include outward bulging of areas lacking membrane-peptidoglycan bonds, the capacity to upregulate vesicle production without also losing outer membrane integrity, enrichment or exclusion of certain proteins and lipids, and membrane fission without direct energy from ATP/GTP hydrolysis. Comparisons of similar budding mechanisms from diverse biological domains have provided new insight into evaluating mechanisms for outer membrane vesiculation.

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Section: Regulation Of Outer Membrane Vesicle Productionmentioning

confidence: 99%

Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles

Kulp

Kuehn

2010

Annu. Rev. Microbiol.

1,218

1,263

View full text Add to dashboard Cite

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“…However, cytoplasmic content, such as genetic material, is also present in MVs (11,12). The levels of MV formation differ depending on the strain and growth conditions, such as variations in temperature, exposure to antibiotics, the presence of oxygen, and nutrient availability (13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation

Fulsundar

Harms

Flaten

et al. 2014

Appl Environ Microbiol

206

197

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dOuter membrane vesicles (OMVs) are continually released from a range of bacterial species. Numerous functions of OMVs, including the facilitation of horizontal gene transfer (HGT) processes, have been proposed. In this study, we investigated whether OMVs contribute to the transfer of plasmids between bacterial cells and species using Gram-negative Acinetobacter baylyi as a model system. OMVs were extracted from bacterial cultures and tested for the ability to vector gene transfer into populations of Escherichia coli and A. baylyi, including naturally transformation-deficient mutants of A. baylyi. Anti-double-stranded DNA (anti-dsDNA) antibodies were used to determine the movement of DNA into OMVs. We also determined how stress affected the level of vesiculation and the amount of DNA in vesicles. OMVs were further characterized by measuring particle size distribution (PSD) and zeta potential. Transmission electron microscopy (TEM) and immunogold labeling were performed using antifluorescein isothiocyanate (anti-FITC)-conjugated antibodies and anti-dsDNA antibodies to track the movement of FITC-labeled and DNA-containing OMVs. Exposure to OMVs isolated from plasmid-containing donor cells resulted in HGT to A. baylyi and E. coli at transfer frequencies ranging from 10 ؊6 to 10 ؊8 , with transfer efficiencies of approximately 10 3 and 10 2 per g of vesicular DNA, respectively. Antibiotic stress was shown to affect the DNA content of OMVs as well as their hydrodynamic diameter and zeta potential. Morphological observations suggest that OMVs from A. baylyi interact with recipient cells in different ways, depending on the recipient species. Interestingly, the PSD measurements suggest that distinct size ranges of OMVs are released from A. baylyi.

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“…In the absence of protein secretion on a medium containing 10 g/l peptone, 20 g/l yeast extract, 5 g/l NaCl, pH 7.5, many small vesicles of about 20 nm in diameter formed in both strains. The results obtained made it possible to conclude for the first time that formation of membrane vesicles is a normal physiological process for this bacterium [Vasilyeva et al, 2008[Vasilyeva et al, , 2009.…”

Section: Formation Of Vesicles In Lysobacter Sp and Their Role In Sementioning

confidence: 85%

“…This indicates the absence of both extracellular muramidase L3 and autolytic muramidase in vesicles. At the same time, vesicles of both strains were found to have an activity of amidase L2 using Abz-Ala-Ala-Phe-pNA (anthranoyl-alanyl-alanyl-phenylalanyl-paranitroanilide) [Vasilyeva et al, 2009].…”

Section: Formation Of Vesicles In Lysobacter Sp and Their Role In Sementioning

confidence: 97%

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The Role of Membrane Vesicles in Secretion of <b><i>Lysobacter</i></b> sp. Bacteriolytic Enzymes

et al. 2013

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Membrane vesicles produced by bacteria have been intensively studied in the recent years. Investigators have noted their roles in essential processes in the bacterial cell including secretion of proteins by the ‘eukaryotic' vesicular mechanism. To date, formation of vesicles is not considered to be a spontaneous event. Many believe it to be a programmed process that can be guided by several mechanisms. Vesicles are derivatives of the cell envelope, which in turn is a supramolecular structure where the functioning and biogenesis of all components are interrelated. Proteins secreted beyond the cell in their translocation are also part of the cell envelope. This also suggests their role in vesicle biogenesis. This review presents the results of vesicle studies in the Gram-negative bacterium Lysobacter sp. This bacterium is of interest as it secretes a number of proteins to the environment, including bacteriolytic enzymes. Bacteriolytic enzymes, on the one hand, are important for studies from a medical point of view as they can form the basis of new generation antimicrobial means. On the other hand, they are a convenient subject for studies of vesicle functions in the vital activities of the bacterial cell.

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Outer membrane vesicles of Lysobacter sp.

Cited by 14 publications

References 6 publications

Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles

Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles

Gene Transfer Potential of Outer Membrane Vesicles of Acinetobacter baylyi and Effects of Stress on Vesiculation

The Role of Membrane Vesicles in Secretion of <b><i>Lysobacter</i></b> sp. Bacteriolytic Enzymes

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