Outer membrane vesicles of Gallibacterium anatis induce protective immunity in egg-laying hens

Pors, Susanne Elisabeth; Pedersen, Ida J.; Skjerning, Ragnhild Bager; Thøfner, Ida; Persson, Gry; Bojesen, Anders Miki

doi:10.1016/j.vetmic.2016.09.021

Cited by 11 publications

(11 citation statements)

References 26 publications

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“…Our data showed that none of the immunization strategies employed was able to successfully prevent A. pleuropneumoniae colonization or the development of lesions associated with infection. This may be due to different reasons, and our data, together with previously published studies [ 11 , 25 , 35 ], suggest caution in dismissing the selected immunogens as simply ineffective for immunization. As already mentioned, A. pleuropneumoniae possess several resistance mechanisms that allow it to avoid clearance by the host immune system.…”

Section: Discussionmentioning

confidence: 51%

“…Furthermore, thanks to their non-live nature, OMVs can be employed in vaccine formulations without incurring in the usual risks associated with live vaccines [ 29 ]. Notably, both autologous and heterologous OMVs have been reported to function as an adjuvant for co-administered recombinant antigens [ 32 – 34 ], but more recently some controversy has emerged regarding the possibility of combining OMVs with other immunogens [ 35 ]. To verify the potential of OMVs as adjuvants, we decided to combine OMVs and recombinant proteins ApfA and VacJ as a third strategy of immunization.…”

Section: Introductionmentioning

confidence: 99%

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In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Antenucci

Fougeroux

Deeney

et al. 2018

Vet Res

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Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A. pleuropneumoniae, as the majority of Gram-negative bacteria, excrete vesicles from its outer membrane (OM), accordingly defined as outer membrane vesicles (OMVs). Thanks to their antigenic similarity to the OM, OMVs have emerged as a promising tool in vaccinology. In this study we describe the in vivo testing of several vaccine prototypes for the prevention of infection by all known A. pleuropneumoniae serotypes. Previously identified vaccine candidates, the recombinant proteins ApfA and VacJ, administered individually or in various combinations with the OMVs, were employed as vaccination strategies. Our data show that the addition of the OMVs in the vaccine formulations significantly increased the specific IgG titer against both ApfA and VacJ in the immunized animals, confirming the previously postulated potential of the OMVs as adjuvant. Unfortunately, the antibody response raised did not translate into an effective protection against A. pleuropneumoniae infection, as none of the immunized groups following challenge showed a significantly lower degree of lesions than the controls. Interestingly, quite the opposite was true, as the animals with the highest IgG titers were also the ones bearing the most extensive lesions in their lungs. These results shed new light on A. pleuropneumoniae pathogenicity, suggesting that antibody-mediated cytotoxicity from the host immune response may play a central role in the development of the lesions typically associated with A. pleuropneumoniae infections.

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Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Antenucci

Fougeroux

Deeney

et al. 2018

Vet Res

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“…G. anatis potentially releases hemagglutinin in OMVs, which is capable of agglutinating avian erythrocytes (Zepeda et al 2009;Ramirez-Apolinar et al 2012;Bager, Persson, et al 2013;Johnson et al 2013;Bager et al 2014). OMVs from G. anatis induce a considerable immune response (Pors, Pedersen, et al 2016 (Lintermans et al 1991;Bougu enec & Bertin 1999). The fimbria protein (FlfA) is very important for virulence in vivo and it was demonstrated that an flfA knockout mutant (DflfA) was considerably attenuated in the natural chicken host without adherence properties ).…”

Section: Virulence Factors Of G Anatismentioning

confidence: 99%

Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofGallibacterium anatisinfection in poultry: a review

Krishnegowda

Dhama

Kumar

et al. 2020

Veterinary Quarterly

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Gallibacterium anatis is a Gram-negative bacterium of the Pasteurellaceae family that resides normally in the respiratory and reproductive tracts in poultry. It is a major cause of oophoritis, salpingitis, and peritonitis, decreases egg production and mortality in hens thereby severely affecting animal welfare and overall productivity by poultry industries across Europe, Asia, America, and Africa. In addition, it has the ability to infect wider host range including domesticated and free-ranging avian hosts as well as mammalian hosts such as cattle, pigs and human. Evaluating the common virulence factors including outer membrane vesicles, fimbriae, capsule, metalloproteases, biofilm formation, hemagglutinin, and determining novel factors such as the RTX-like toxin GtxA, elongation factor-Tu, and clustered regularly interspaced short palindromic repeats (CRISPR) has pathobiological, diagnostic, prophylactic, and therapeutic significance. Treating this bacterial pathogen with traditional antimicrobial drugs is discouraged owing to the emergence of widespread multidrug resistance, whereas the efficacy of preventing this disease by classical vaccines is limited due to its antigenic diversity. It will be necessary to acquire in-depth knowledge on important virulence factors, pathogenesis and, concerns of rising antibiotic resistance, improvised treatment regimes, and novel vaccine candidates to effectively tackle this pathogen. This review substantially describes the etio-epidemiological aspects of G. anatis infection in poultry, and updates the recent development in understanding the pathogenesis, organism evolution and therapeutic and prophylactic approaches to counter G. anatis infection for safeguarding the welfare and health of poultry.

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“…Vaccination experiments using either outer membrane vesicles and fimbrial protein FlfA or recombinant Gtx-N' (N-terminal half of GtxA) and Gtx-C' (C-terminal half of GtxA) together with FlfA resulted in protection against challenge with heterologous strains of G. anatis. This demonstrates the potential of GtxA in the development of novel vaccines against G. anatis infections in poultry (Table 1) [111,[120][121][122].…”

Section: Gtxa Martx From the Poultry Pathogen Gallibacterium Anatismentioning

confidence: 78%

RTX Toxins of Animal Pathogens and Their Role as Antigens in Vaccines and Diagnostics

Frey

2019

Toxins

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Exotoxins play a central role in the pathologies caused by most major bacterial animal pathogens. The large variety of vertebrate and invertebrate hosts in the animal kingdom is reflected by a large variety of bacterial pathogens and toxins. The group of repeats in the structural toxin (RTX) toxins is particularly abundant among bacterial pathogens of animals. Many of these toxins are described as hemolysins due to their capacity to lyse erythrocytes in vitro. Hemolysis by RTX toxins is due to the formation of cation-selective pores in the cell membrane and serves as an important marker for virulence in bacterial diagnostics. However, their physiologic relevant targets are leukocytes expressing β2 integrins, which act as specific receptors for RTX toxins. For various RTX toxins, the binding to the CD18 moiety of β2 integrins has been shown to be host specific, reflecting the molecular basis of the host range of RTX toxins expressed by bacterial pathogens. Due to the key role of RTX toxins in the pathogenesis of many bacteria, antibodies directed against specific RTX toxins protect against disease, hence, making RTX toxins valuable targets in vaccine research and development. Due to their specificity, several structural genes encoding for RTX toxins have proven to be essential in modern diagnostic applications in veterinary medicine.

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Outer membrane vesicles of Gallibacterium anatis induce protective immunity in egg-laying hens

Cited by 11 publications

References 26 publications

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae

Etiology, epidemiology, pathology, and advances in diagnosis, vaccine development, and treatment ofGallibacterium anatisinfection in poultry: a review

RTX Toxins of Animal Pathogens and Their Role as Antigens in Vaccines and Diagnostics

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