Outer membrane protein subtypes and investigation of recurrent Haemophilus influenzae type b disease

Edmonson, M. Bruce; Granoff, Dan M.; Barenkamp, Stephen J.; Chesney, P. Joan

doi:10.1016/s0022-3476(82)80635-5

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…Haemophilus influenza type b is the most common cause of bacterial meningitis in children in the United States. Outer membrane protein (OMP) profiles (4,6,13,22), serum bactericidal assays with cross-adsorbed antisera (1), and biotype analyses (19) have been used to distinguish among type b isolates in an effort to assess the heterogeneity of these organisms as well as to provide important epidemiologic information on the transmission of this organism. In the United States, more than 94% of the type b isolates from children with bacteremic disease are biotype I (13), and approximately 44% have a common OMP subtype, 1H (13).…”

mentioning

confidence: 99%

Lipopolysaccharide gel profiles of Haemophilus influenzae type b are not stable epidemiologic markers

Tolan¹,

Munson²,

Granoff³

1986

J Clin Microbiol

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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of lipopolysaccharide (LPS) was performed to assess the usefulness of this technique for the epidemiologic analysis of Haemophilus influenza type b isolates. LPS samples were prepared from isolates which had been passaged either in vitro or in infant rats. Preparations from paired isolates from a number of epidemiologically related clinical situations also were examined. The gel patterns of LPS prepared on different occasions from an individual isolate were stable. However, the LPS gel patterns changed in 5 of 14 (36%) of the passaged isolates, and differences in gel patterns also were observed among epidemiologically related isolates. The variability in LPS electrophoretic patterns of individual isolates indicated that this technique is not useful for the epidemiologic analysis of H. influenzae type b disease.

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mentioning

confidence: 99%

Lipopolysaccharide gel profiles of Haemophilus influenzae type b are not stable epidemiologic markers

Tolan¹,

Munson²,

Granoff³

1986

J Clin Microbiol

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“…To date, 21 different outer membrane protein subtypes have been identified, but 5 subtypes account for more than 90% of the isolates from patients hospitalized with invasive diseases in 22 states (9). This subtyping system has been proven useful for epidemiological studies of Haemophilus type b disease (2,Sa,7,9).…”

mentioning

confidence: 99%

Comparison of outer membrane protein subtypes of Haemophilus influenzae type b isolates from healthy children in the general population and from diseased patients

Hampton¹,

Barenkamp²,

Granoff³

1983

J Clin Microbiol

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Over a 12-month period we obtained throat cultures from 1,448 children less than 5 years of age attending well-child clinics and identified 24 carriers of Haemophilus influenzae type b (1.7%). The outer membrane protein subtypes of the strains from the carriers were compared to the subtypes of isolates from 50 patients with Haemophilus type b disease hospitalized in St. Louis, Mo., during the same period (1981 to 1982), and the latter were compared to the subtypes of isolates from 51 patients hospitalized between 1977 and 1980. There were no significant differences in the frequencies of the five most common subtypes (1L, 1H, 2L, 2H, and 3L), comparing isolates from the carriers to those from the patients. However, 5 of the 24 throat isolates had the unusual 13L subtype compared with only 1 of the 50 invasive isolates (P = 0.02). The lower frequency of 13L strains among the invasive isolates suggests that type b isolates with this subtype may be less pathogenic than type b isolates with other subtypes. Subtype 2L strains accounted for only 2% of recent cerebrospinal fluid or blood isolates, compared with 22% of those from 1977 to 1980 (P = 0.02). Subtype 1H and 3L strains together accounted for 73%, compared with 47% of the earlier ones (P = 0.02). Thus, temporal shifts may also occur in the subtype distribution of Haemophilus type b strains causing invasive disease in a community.

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“…They suggested that a two-dose regimen should be considered for children \15 months old who are recovering from an episode of invasive Hib disease [12]. Hib conjugate vaccine is immunogenic in children with no anti-PRP response to invasive Hib disease, because children are most at risk of developing a second episode of Hib invasive disease within 6 months of the initial illness [7]. Indeed, our study included 5 children who experienced recurrent episodes of invasive Hib disease.…”

Section: Discussionmentioning

confidence: 99%

“…Children \2 years old may not develop protective antibodies to PRP after episodes of invasive Hib disease [6]. Furthermore, a subpopulation of children who have recovered from invasive Hib disease may also be at risk of developing a second episode of invasive Hib disease [7]. Strategies aimed at preventing a second episode of Hib disease in children with a history of Hib disease have included immunization with Hib conjugate vaccine.…”

Section: Introductionmentioning

confidence: 99%

Anti-polyribosylribitol phosphate antibody in pediatric patients with Haemophilus influenzae type b invasive disease

Ishiwada

Honda

Tanaka

et al. 2011

Journal of Infection and Chemotherapy

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Outer membrane protein subtypes and investigation of recurrent Haemophilus influenzae type b disease

Cited by 18 publications

References 32 publications

Lipopolysaccharide gel profiles of Haemophilus influenzae type b are not stable epidemiologic markers

Lipopolysaccharide gel profiles of Haemophilus influenzae type b are not stable epidemiologic markers

Comparison of outer membrane protein subtypes of Haemophilus influenzae type b isolates from healthy children in the general population and from diseased patients

Anti-polyribosylribitol phosphate antibody in pediatric patients with Haemophilus influenzae type b invasive disease

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