Outer Membrane Porin F in E. coli Is Critical for Effective Predation by
            <i>Bdellovibrio</i>

Mun, Wonsik; Upatissa, Sumudu; Lim, Sungbin; Dwidar, Mohammed; Mitchell, Robert J.

doi:10.1128/spectrum.03094-22

Cited by 5 publications

(4 citation statements)

References 28 publications

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“…Alternatively, besides allowing bile salts to pass through, OmpF could also be disadvantageous because it is a receptor for colicins and phages ( 41 – 45 ). Bdellovibrio bacteriovorus , present in the gut of animals and healthy humans ( 46 ), uses OmpF to recognize and attack E. coli ( 47 ). However, it is unlikely that an obligate aerobe such as Bdellovibrio predominates in the streptomycin-treated mouse gut.…”

Section: Discussionmentioning

confidence: 99%

OmpC-Dependent Bile Tolerance Contributes to E. coli Colonization of the Mammalian Intestine

Doranga

Conway

2023

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

OmpC-Dependent Bile Tolerance Contributes to E. coli Colonization of the Mammalian Intestine

Doranga

Conway

2023

Microbiol Spectr

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“…5C ), one might postulate that the expression of ompF2 during plant colonization may allow diffusion of toxic molecules within the bacteria and may impair bacterial growth. Interestingly, OmpF has been proposed to be recognized by the host ( 59 – 61 ). Hence, one may also postulate that ompF2 repression evades host recognition.…”

Section: Discussionmentioning

confidence: 99%

Emergence of the Dickeya genus involved duplication of the OmpF porin and the adaptation of the EnvZ-OmpR signaling network

Cochard,

Caby,

Gruau

et al. 2023

Microbiol Spectr

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Genome evolution, and more specifically gene duplication, is a key process shaping host–microorganism interaction. The conserved paralogs usually provide an advantage to the bacterium to thrive. If not, these genes become pseudogenes and disappear. Here, we show that during the emergence of the genus Dickeya , the gene encoding the porin OmpF was duplicated. Our results show that the ompF2 expression is deleterious to the virulence of Dickeya dadantii , the agent causing soft rot disease. Interestingly, ompF 2 is regulated while ompF is constitutive but activated by the EnvZ-OmpR two-component system. In vitro , acidic pH triggers the system. The pH measured in four eudicotyledons increased from an initial pH of 5.5 to 7 within 8 h post-infection. Then, the pH decreased to 5.5 at 10 h post-infection and until full maceration of the plant tissue. Yet, the production of phenolic acids by the plant’s defenses prevents the activation of the EnvZ-OmpR system to avoid the ompF2 expression even though environmental conditions should trigger this system. We highlight that gene duplication in a pathogen is not automatically an advantage for the infectious process and that, there was a need for our model organism to adapt its genetic regulatory networks to conserve these duplicated genes. IMPORTANCE Dickeya species cause various diseases in a wide range of crops and ornamental plants. Understanding the molecular program that allows the bacterium to colonize the plant is key to developing new pest control methods. Unlike other enterobacterial pathogens, Dickeya dadantii , the causal agent of soft rot disease, does not require the EnvZ-OmpR system for virulence. Here, we showed that during the emergence of the genus Dickeya , the gene encoding the porin OmpF was duplicated and that the expression of ompF2 was deleterious for virulence. We revealed that while the EnvZ-OmpR system was activated in vitro by acidic pH and even though the pH was acidic when the plant is colonized, this system was repressed by phenolic acid (generated by the plant’s defenses). These results provide a unique— biologically relevant—perspective on the consequence of gene duplication and the adaptive nature of regulatory networks to retain the duplicated gene.

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“…Strains susceptible to predation have not conclusively been shown to become resistant via mutation, although manual deletion of the OmpF porin affected predation kinetics (an effect particular to E. coli prey, not demonstrated to be a direct effect at present, [55]). Conversely, a loss-of-function screen identified mutation of secretion system genes as conveying predation susceptibility to a previously resistant Acidovorax citrulli [56].…”

Section: Relevance In the Era Of Amrmentioning

confidence: 99%

“…The acquisition and carriage of antimicrobial resistance genes by prey does not deleteriously effect predation by Bdellovibrio [ 53 ], although usage of the potential barrier of an S-layer can partly protect prey [ 54 ]. Strains susceptible to predation have not conclusively been shown to become resistant via mutation, although manual deletion of the OmpF porin affected predation kinetics (an effect particular to E. coli prey, not demonstrated to be a direct effect at present, [ 55 ]). Conversely, a loss-of-function screen identified mutation of secretion system genes as conveying predation susceptibility to a previously resistant Acidovorax citrulli [ 56 ].…”

Section: Relevance In the Era Of Amrmentioning

confidence: 99%

Moving toward a better understanding of the model bacterial predator Bdellovibrio bacteriovorus

Caulton

Lovering

2023

Microbiology

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The bacterial predator Bdellovibrio bacteriovorus is a model for the wider phenomenon of bacteria:bacteria predation, and the specialization required to achieve a lifestyle dependent on prey consumption. Bdellovibrio bacteriovorus is able to recognize, enter and ultimately consume fellow Gram-negative bacteria, killing these prey from within their periplasmic space, and lysing the host at the end of the cycle. The classic phenotype-driven characterization (and observation of predation) has benefitted from an increased focus on molecular mechanisms and fluorescence microscopy and tomography, revealing new features of several of the lifecycle stages. Herein we summarize a selection of these advances and describe likely areas for exploration that will push the field toward a more complete understanding of this fascinating ‘two-cell’ system.

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Outer Membrane Porin F in E. coli Is Critical for Effective Predation by Bdellovibrio

Cited by 5 publications

References 28 publications

OmpC-Dependent Bile Tolerance Contributes to E. coli Colonization of the Mammalian Intestine

OmpC-Dependent Bile Tolerance Contributes to E. coli Colonization of the Mammalian Intestine

Emergence of the Dickeya genus involved duplication of the OmpF porin and the adaptation of the EnvZ-OmpR signaling network

Moving toward a better understanding of the model bacterial predator Bdellovibrio bacteriovorus

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