Outdoor airborne fungi captured by viable and non-viable methods

Fernández-Rodríguez, Santiago; Maya‐Manzano, José María; Silva-Palacios, Inmaculada; Gonzalo-Garijo, Ángela

doi:10.1016/j.funeco.2013.11.004

Cited by 38 publications

(20 citation statements)

References 57 publications

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“…The daily average total spore concentration of 3,608 ± 3,182 spores m -3 noted in the present study is comparable to that described in some European studies (Docampo et al, 2011;Fernández-Rodríguez et al, 2014). However, our recorded concentrations were lower than those found in a study conducted in Puerto Rico (Quintero et al, 2010), where the mean concentration in the peak month (September) reached to nearly 30,000 spores m -3 , (our peak data was 8,126 spores m -3 in June).…”

Section: Discussionsupporting

confidence: 91%

“…These data provide information on temporal variation (i.e., daily, diurnal, and seasonal variations) (Liao and Luo, 2005;Yamamoto et al, 2012;Fernández-Rodríguez et al, 2014), spatial variation (Chow et al, 2015), and the predictive land-use regression (LUR) models (Kallawicha et al, 2015). In the United States, the concentrations of ambient fungal spores and other aeroallergens (e.g., grass, weed, and pollen) are provided by the National Allergy Bureau of the American Academy of Allergy, Asthma & Immunology on a daily basis as open access data on the Internet, which can be used as a guideline by the residents in the monitored areas, particularly the susceptible group, for avoiding or minimizing exposure (National Allergy Bureau, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ambient Fungal Spore Concentration in a Subtropical Metropolis: Temporal Distributions and Meteorological Determinants

Kallawicha

Chen

Chao

et al. 2017

Aerosol Air Qual. Res.

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Ambient particles comprise approximately 25% of fungal spores, which cause adverse health outcomes such as respiratory diseases, allergy, and infection. In this study, we investigated temporal variations and distributions of ambient fungal spores in an urban area of the Taipei metropolis for over 1 year. A Burkard 7-day volumetric spore trap was used to collect air samples. Samples collected daily were stained, counted, and identified on the basis of morphological characteristics. The associations between fungal spores and environmental parameters were then evaluated through multiple regression analysis. Daily monitoring data revealed a large variation in fungal spore concentrations. Specifically, fungal spores peaked during summer months (June-August) and declined during winter months (December-early March); moreover, the average concentration of total fungal spores was 3,607.97 ± 3,181.81 spores m -3 . Ascospores were the most prevalent taxon that was recovered from the samples, followed by basidiospores, Aspergillus/Penicillium, and Cladosporium. Multiple regression analysis revealed that meteorological parameters were the main predictors of fungal concentrations. Temperature, wind speed, and humidity were consistently correlated with total fungi and major fungal taxa, and sunlight had a negative association with ascospores. Among the atmospheric pollutants, particulate matter with an aerodynamic diameter ≤ 10 µm (PM 10 ) and ozone were positively associated with fungal spores. Carbon monoxide (CO) at lag day 1 had a negative association with basidiospores. This is the first study to characterize daily concentrations and determinants of ambient fungal spores in an urban area of Taipei metropolis. The obtained data can be used to evaluate the health impact of fungal spore exposure on the residents of the Taipei metropolitan area.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Ambient Fungal Spore Concentration in a Subtropical Metropolis: Temporal Distributions and Meteorological Determinants

Kallawicha

Chen

Chao

et al. 2017

Aerosol Air Qual. Res.

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“…It is also possible to link our spore data from outdoor air to many other studies that used microscopic enumeration. For example, our median spore levels (outdoor: 69 × 10 3 m −3 ) were many folds higher than that published by Li and Kendrick in samples from outside residences in Kitchner‐Waterloo (Ontario, Canada): 2.9 × 10 3 m −3 ; Codina and colleagues in outdoor samples from Tampa (Florida, URA): 2.4 × 10 3 m −3 , Fernandez‐Rodriguez et al in outdoor samples from Badajoz (Spain): 0.09‐10 × 10 3 m −3 and Jara et al in samples from outdoor in North American Midwest: 9 × 10 3 m −3 . However, our data are comparable with the ranges reported by Gots et al when reviewing studies that covered various regions in United States: 0.4‐80 × 10 3 m −3 and Reponen and co‐workers in New Orleans and Ohio (1.1‐8.4 × 10 3 m −3 ) .…”

Section: Discussioncontrasting

confidence: 68%

Fungal aerosol composition in moldy basements

2019

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Experimental aerosolization studies revealed that fungal fragments including small fragments in the submicrometer size are released from fungal cultures and have been suggested to represent an important fraction of overall fungal aerosols in indoor environments. However, their prevalence indoors and outdoors remains poorly characterized. Moldy basements were investigated for airborne fungal particles including spores, submicron fragments, and larger fragments. Particles were collected onto poly‐L‐lysine‐coated polycarbonate filters and qualitatively and quantitatively analyzed using immunogold labeling combined with field emission scanning electron microscopy. We found that the total fungal aerosol levels including spores, submicrometer, and larger fragments in the moldy basements (median: 80 × 103 m−3) were not different from that estimated in control basements (63 × 103 m−3) and outdoor (90 × 103 m−3). However, mixed effect modeling of the fungal aerosol composition revealed that the fraction of fragments increased significantly in moldy basements, versus the spore fraction that increased significantly in outdoor air. These findings provide new insight on the compositional variation of mixed fungal aerosols in indoor as compared to outdoor air. Our results also suggest that further studies, aiming to investigate the role of fungal aerosols in the fungal exposure‐disease relationships, should consider the mixed composition of various types of fungal particles.

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“…Although aerosol microbial diversity can depend on different factors including the mechanism of the aerosol collector used (Fernández-Rodríguez et al, 2014), the combination of the BioSampler system, filtration through a nitrocellulose membrane and subsequent chaotropic solid-phase DNA extraction, lead to the isolation of amplifiable DNA in the same way as in by previous studies (Fierer et al, 2008;Kembel et al, 2012). This DNA extraction procedure also permitted the microbial DNA analysis of the statue surface, showing a rich and surprising microbial community.…”

Section: Discussionmentioning

confidence: 94%

Biodeterioration of epoxy resin: a microbial survey through culture‐independent and culture‐dependent approaches

Pangallo

Bučková

Kraková

et al. 2014

Environmental Microbiology

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During the 20th century, synthetic polymers were greatly used in the field of art. In particular, the epoxy resins were used for both conservation and for creating sculptures. The biodeterioration of these polymers has not been adequately studied. The aim of this investigation was to examine the microflora responsible for the deterioration of an epoxy statue exposed to outdoor conditions. Fungal and bacterial microflora were isolated from the art object, clustered by fluorescence-ITS (internal transcribed spacer), identified by ITS and 16S rRNA sequencing and tested for their lipolytic abilities by three agar assays. Different algal, bacterial, cyanobacterial and fungal clone libraries were constructed. The surrounding airborne microflora was analyzed using culture-dependent and culture-independent approaches. The results indicated the presence, on the statue surface, of an interesting and differentiate microbial community composed of rock-inhabiting members, algal photobionts (Trebouxia spp., Chloroidium ellipsoideum and Chlorella angustoellipsoidea), Cyanobacteria (Leptolyngbya sp., Phormidium sp., Cylindrospermum stagnale, Hassallia byssoidea and Geitlerinema sp.), black yeasts related to the species Friedmanniomyces endolithicus, Pseudotaeniolina globosa, Phaeococcomyces catenatus and Catenulostroma germanicum and several plant-associated fungi. This investigation provides new information on the potential microfloral inhabitants of epoxy resin discovering a new ecological niche, occupied mainly by several members of rock-colonizing microbial species.

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Outdoor airborne fungi captured by viable and non-viable methods

Cited by 38 publications

References 57 publications

Ambient Fungal Spore Concentration in a Subtropical Metropolis: Temporal Distributions and Meteorological Determinants

Ambient Fungal Spore Concentration in a Subtropical Metropolis: Temporal Distributions and Meteorological Determinants

Fungal aerosol composition in moldy basements

Biodeterioration of epoxy resin: a microbial survey through culture‐independent and culture‐dependent approaches

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