Outcrossing rate in olive assessed by microsatellite and inter simple sequence repeat (ISSR) markers

Souza, Rafaeli Aparecida Vieira de

doi:10.5897/ajb11.2191

Afr. J. Biotechnol.

2012

DOI: 10.5897/ajb11.2191

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Outcrossing rate in olive assessed by microsatellite and inter simple sequence repeat (ISSR) markers

Rafaeli Aparecida Vieira de Souza¹

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2015

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Genetic relationships analysis of olive cultivars grown in China

Zhan¹,

Cheng²,

Su³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples were collected from Xichang. Five of these cultivars were issued from a Chinese breeding program. Genomic DNA samples were extracted from young leaves and PCR was used to generate SSR and ISSR markers. A total of 107 polymorphic bands were detected on thirteen SSR loci, with an average of eight alleles per locus. The observed heterozygosity ranged from 0.785 (DCA03) to 0.990 (GAPU47), and the expected heterozygosity varied between 0.782 (DCA03) and 0.940 (GAPU103A). The discrimination power ranged from 0.57 to 0.83, while the polymorphism information content values ranged from 0.768 (DCA03) to 0.934 (GAPU103A). Nine ISSR primers generated 85 reproducible bands of which 78 (91.8%) were polymorphic. Based 5959 Genetic similarities among olive cultivars in China ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 5958-5969 (2015) on our data, genetic similarity between cultivars ranged from 0.57 to 0.83. Cluster analysis revealed that 32 cultivars were clustered into six groups, which supports similar morphology such as use, oil content and fruit weight but not similar geographical origins. Our data also allow the identification of unknown cultivars and cases of synonyms.

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Genetic relationships analysis of olive cultivars grown in China

Zhan¹,

Cheng²,

Su³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

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Outcrossing rate in olive assessed by microsatellite and inter simple sequence repeat (ISSR) markers

Cited by 1 publication

References 24 publications

Genetic relationships analysis of olive cultivars grown in China

Genetic relationships analysis of olive cultivars grown in China

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