Outcome of oral infection in mice inoculated with Trypanosoma cruzi IV of the Western Brazilian Amazon

Teston, Ana Paula Margioto; Abreu, Ana Paula de; Abegg, Camila Piva; Gomes, Mônica Lúcia; Toledo, Max Jean de Ornelas

doi:10.1016/j.actatropica.2016.11.019

Cited by 18 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data show that the qPCR technique, in addition to quantifying parasite load, is a powerful tool for the detection of T. cruzi DNA, both in the diagnosis and in the monitoring of cure after etiological treatment of CD. The results of qPCR in mice infected with a TcII strain in this study confirm the findings of Teston et al [27], who described the greater sensitivity of qPCR over cPCR in mice orally inoculated with four strains belonging to another T. cruzi DTU (TcIV). In the present study, 1 × 10 4 BT forms were used for the oral inoculation of animals; the parasitemia levels of the untreated animals were 40X higher than those observed in mice inoculated by the same route and with the same strain, but using a greater inoculum of 2 × 10 6 metacyclic trypomastigotes derived from culture in LIT medium [21].…”

Section: Discussionsupporting

confidence: 91%

“…Our group has demonstrated that the oral infection of mice with different strains of T. cruzi (TcI, TcII and TcIV) is more severe than the infection by the intraperitoneal route using the same inoculation dose [26,27], and has a worse response to treatment with BZ [unpublished data]. Despite the promising results obtained in vitro, studies on the in vivo trypanocidal action of EOs are scarce and a literature review shows that only two publications by our group have evaluated these compounds in mice infected with T. cruzi.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

Sarto

Silva

Fernandes

et al. 2021

BMC Complement Med Ther

Self Cite

View full text Add to dashboard Cite

Background Trypanosoma cruzi is the etiological agent of Chagas disease (CD) or American trypanosomiasis, an important public health problem in Latin America. Benznidazole (BZ), a drug available for its treatment, has limited efficacy and significant side effects. Essential oils (EOs) have demonstrated trypanocidal activity and may constitute a therapeutic alternative. Our aim was to evaluate the efficacy of the EOs of clove (CEO - Syzygium aromaticum) and ginger (GEO - Zingiber officinale), administered alone and in combination with BZ, in Swiss mice infected with T. cruzi. Methods The animals were inoculated with 10,000 blood trypomastigotes of the Y strain of T. cruzi II by gavage and divided into four groups (n = 12 to 15): 1) untreated control (NT); 2) treated with BZ; 3) treated with CEO or GEO; and 4) treated with BZ + CEO or GEO. The treatments consisted of oral administration of 100 mg/kg/day, from the 5th day after parasite inoculation, for 20 consecutive days. All groups were submitted to fresh blood examination (FBE), blood culture (BC), conventional PCR (cPCR) and real-time PCR (qPCR), before and after immunosuppression with cyclophosphamide. Results Clove and ginger EOs, administered alone and in combination with BZ, promoted suppression of parasitemia (p < 0.0001), except for the animals treated with CEO alone, which presented a parasitemia curve similar to NT animals. However, there was a decrease in the BC positivity rate (p < 0.05) and parasite load (< 0.0001) in this group. Treatment with GEO alone, on the other hand, besides promoting a decrease in the BC positivity rate (p < 0.05) and parasite load (p < 0.01), this EO also resulted in a decrease in mortality rate (p < 0.05) of treated mice. Conclusions Decreased parasite load, as detected by qPCR, was observed in all treatment groups (BZ, CEO, GEO and BZ + EOs), demonstrating benefits even in the absence of parasitological cure, thus opening perspectives for further studies.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

Sarto

Silva

Fernandes

et al. 2021

BMC Complement Med Ther

Self Cite

View full text Add to dashboard Cite

show abstract

“…In endemic areas of the Amazon region of Brazil, and in eastern Colombia and Venezuela, TcIV is considered to be the predominant DTU responsible for most of the acute diseases caused by this DTU (Carrasco et al ., 2012; Guhl and Ramírez, 2013; Segovia et al ., 2013; Monteiro et al ., 2013 a ; 2013 b ; Margioto Teston et al ., 2017). TcIV has also been strongly incriminated with lethal cases relating to oral transmission in Colombia and Brazil (Monteiro et al ., 2013 a ; 2013 b ; Ramírez et al ., 2013; Dario et al ., 2016; Hernández et al ., 2016).…”

Section: T Cruzi Heterogeneity and CCCmentioning

confidence: 99%

A systematic review of the Trypanosoma cruzi genetic heterogeneity, host immune response and genetic factors as plausible drivers of chronic chagasic cardiomyopathy

et al. 2018

View full text Add to dashboard Cite

Chagas disease is a complex tropical pathology caused by the kinetoplastid Trypanosoma cruzi. This parasite displays massive genetic diversity and has been classified by international consensus in at least six Discrete Typing Units (DTUs) that are broadly distributed in the American continent. The main clinical manifestation of the disease is the chronic chagasic cardiomyopathy (CCC) that is lethal in the infected individuals. However, one intriguing feature is that only 30-40% of the infected individuals will develop CCC. Some authors have suggested that the immune response, host genetic factors, virulence factors and even the massive genetic heterogeneity of T. cruzi are responsible of this clinical pattern. To date, no conclusive data support the reason why a few percentages of the infected individuals will develop CCC. Therefore, we decided to conduct a systematic review analysing the host genetic factors, immune response, cytokine production, virulence factors and the plausible association of the parasite DTUs and CCC. The epidemiological and clinical implications are herein discussed.

show abstract

“…The phenotypic characteristics of the two isolates that were utilised in this study are not known, but they are phylogenetically distinct (C8 clone 1 from the TcI DTU and 10R26 from the TcIV DTU) and both have relevance to CD within humans [34,35]. The C8 clone 1 isolate was obtained from a vector within the domestic cycle, and 10R26 was isolated from a monkey in the sylvatic cycle.…”

Section: Parasitesmentioning

confidence: 99%

The effect of reinfection and mixed Trypanosoma cruzi infections on disease progression in mice

et al. 2018

View full text Add to dashboard Cite

The progression of Chagas disease (CD) varies significantly from host to host and is affected by multiple factors. In particular, mixed strain infections and reinfections have the potential to exacerbate disease progression subsequently affecting clinical management of patients with CD. Consequently, an associated reduction in therapeutic intervention and poor prognosis may occur due to this exacerbated disease state. This study investigated the effects of mixed strain infections and reinfection with Trypanosoma cruzi in mice, using two isolates from different discrete typing units, TcI (C8 clone 1) and TcIV (10R26). There were no significant differences in mortality rate, body weight or body condition among mice infected with either C8 clone 1, 10R26, or a mixture of both isolates. However, the parasite was found in a significantly greater number of host organs in mice infected with a mixture of isolates, and the histopathological response to infection was significantly greater in mice infected with C8 clone 1 alone, and C8 clone 1+10R26 mixed infections than in mice infected with 10R26 alone. To investigate the effects of reinfection, mice received either a double exposure to C8 clone 1; a double exposure to 10R26; exposure to C8 clone 1 followed by 10R26; or exposure to 10R26 followed by C8 clone 1. Compared to single infection groups, mortality was significantly increased, while survival time, body weight and body condition were all significantly decreased across all reinfection groups, with no significant differences among these groups. The mortality rate over all reinfection groups was 63.6%, compared to 0% in single infection groups, however there was no evidence of a greater histopathological response to infection. These results suggest firstly, that the C8 clone 1 isolate is more virulent than the 10R26 isolate, and secondly, that a more disseminated infection may occur with a mixture of isolates than with single isolates, although there is no evidence that mixed infections have a greater pathological effect. By contrast, reinfections do have major effects on host survivability and thus disease outcome. This confirms previous research demonstrating spontaneous deaths following reinfection, a phenomenon that to our knowledge has only been reported once before.

show abstract

Outcome of oral infection in mice inoculated with Trypanosoma cruzi IV of the Western Brazilian Amazon

Cited by 18 publications

References 23 publications

Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

A systematic review of the Trypanosoma cruzi genetic heterogeneity, host immune response and genetic factors as plausible drivers of chronic chagasic cardiomyopathy

The effect of reinfection and mixed Trypanosoma cruzi infections on disease progression in mice

Contact Info

Product

Resources

About