Outcome of Conventional Adipose Tissue Grafting for Contour Deformities of Face and Role of Ex Vivo Expanded Adipose Tissue-Derived Stem Cells in Treatment of Such Deformities

Bashir, Muhammad Mustehsan; Sohail, Muhammad; Bashir, Afzaal; Khan, Farid Ahmad; Jan, Sadia Nosheen; Imran, Muhammad; Ahmad, Fridoon Jawad; Choudhery, Mahmood S

doi:10.1097/scs.0000000000004367

Cited by 18 publications

(26 citation statements)

References 32 publications

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“…Adipose tissue (47.26 ± 5.6 cc) was collected by tumescent liposuction as described by us previously . Briefly, after sterilization of skin surface, tumescent solution (0.9% saline, lidocaine, epinephrine) was infiltrated into donor sites.…”

Section: Methodsmentioning

confidence: 99%

Stromal vascular fraction‐enriched platelet‐rich plasma therapy reverses the effects of androgenetic alopecia

Butt

Hussain

Ahmad

et al. 2019

J of Cosmetic Dermatology

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Background Since antiquity, humans have been trying to devise remedies to cure androgenetic alopecia (AGA). These efforts include use of oral and topical concoctions and hair transplant strategies. As AGA affects people of all colors and creed, there has been a continuous effort to find a magic bullet against AGA. Unfortunately, to date, all the strategies to negate AGA effects have limitations and thus require new treatment options. Aim To evaluate the efficacy of use of stromal vascular fraction (SVF) in androgenetic alopecia patients. Methods Stromal vascular fraction was obtained by enzymatic digestion of autologous adipose tissue. The patients were divided into two groups, that is, platelet‐rich plasma (PRP) group and SVF‐PRP group. In PRP group, only PRP was injected, while in SVF‐PRP group a mixture of PRP and SVF was injected in affected scalp areas. After two sessions (4 weeks apart), the patients in both groups were assessed and analyzed using various parameters. Results Mean hair density in PRP group was increased from 52.44 hair/cm2 to 63.72 hair/cm2 (21.51% increase); while in SVF‐PRP group, it was 37.66 hair/cm2 before treatment and 57.11 hair/cm2 after SVF‐PRP therapy (51.64% increase). Percentage reduction in pull test was more significant in SVF‐PRP group (80.78 ± 5.84) as compared to PRP group (34.01 ± 22.44). The physician and patient assessment scores also indicated a significant improvement in SVF‐PRP group. Conclusion A combined SVF‐PRP therapy reversed effects of AGA more efficiently as compared to PRP therapy alone.

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Section: Methodsmentioning

confidence: 99%

Stromal vascular fraction‐enriched platelet‐rich plasma therapy reverses the effects of androgenetic alopecia

Butt

Hussain

Ahmad

et al. 2019

J of Cosmetic Dermatology

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Section: Introductionmentioning

confidence: 99%

“…Autologous adipose tissue grafting has been a widely accepted surgical tool for anti-aging cosmetics (Charles-De-Sá et al, 2015) and reconstructive restoration of various congenital or acquired facial soft tissue deformities (Bashir et al, 2018). However, conventional fat grafting procedure needs to be repeated multiple times to achieve satisfactory results (Bashir et al, 2018), which may be associated with the low graft survival rate and poor revascularization (Ma et al, 2015). To overcome these two limitations, recent scholars propose to combine with additional autologous adipose-derived stem cells (ASCs) which have the ability to differentiate into mature adipocytes to supplement apoptotic cells and secrete angiogenic growth factors to enhance angiogenesis (Bashir et al, 2018; Kotaro et al, 2008; Philips, Marra & Rubin, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…However, conventional fat grafting procedure needs to be repeated multiple times to achieve satisfactory results (Bashir et al, 2018), which may be associated with the low graft survival rate and poor revascularization (Ma et al, 2015). To overcome these two limitations, recent scholars propose to combine with additional autologous adipose-derived stem cells (ASCs) which have the ability to differentiate into mature adipocytes to supplement apoptotic cells and secrete angiogenic growth factors to enhance angiogenesis (Bashir et al, 2018; Kotaro et al, 2008; Philips, Marra & Rubin, 2014). The clinical trials also confirm that supplementation of ASCs to adipose grafts is superior to conventional lipoinjection for facial recontouring (Bashir et al, 2018; Kotaro et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…To overcome these two limitations, recent scholars propose to combine with additional autologous adipose-derived stem cells (ASCs) which have the ability to differentiate into mature adipocytes to supplement apoptotic cells and secrete angiogenic growth factors to enhance angiogenesis (Bashir et al, 2018; Kotaro et al, 2008; Philips, Marra & Rubin, 2014). The clinical trials also confirm that supplementation of ASCs to adipose grafts is superior to conventional lipoinjection for facial recontouring (Bashir et al, 2018; Kotaro et al, 2008). Nevertheless, the use of autologous ASCs has not been FDA-approved.…”

Section: Introductionmentioning

confidence: 99%

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Crucial lncRNAs associated with adipocyte differentiation from human adipose-derived stem cells based on co-expression and ceRNA network analyses

Chen

Xie

Jin

2019

PeerJ

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Background Injection of adipose-derived stem cells (ASCs) is a promising treatment for facial contour deformities. However, its treatment mechanisms remain largely unknown. The study aimed to explain the molecular mechanisms of adipogenic differentiation from ASCs based on the roles of long noncoding RNAs (lncRNAs). Methods Datasets of mRNA–lncRNA (GSE113253) and miRNA (GSE72429) expression profiling were collected from Gene Expression Omnibus database. The differentially expressed genes (DEGs), lncRNAs (DELs) and miRNAs (DEMs) between undifferentiated and adipocyte differentiated human ASCs were identified using the Linear Models for Microarray Data method. DELs related co-expression and competing endogenous RNA (ceRNA) networks were constructed. Protein–protein interaction (PPI) analysis was performed to screen crucial target genes. Results A total of 748 DEGs, 17 DELs and 51 DEMs were identified. A total of 13 DELs and 279 DEGs with Pearson correlation coefficients > 0.9 and p-value < 0.01 were selected to construct the co-expression network. A total of 151 interaction pairs among 112 nodes (10 DEMs; eight DELs; 94 DEGs) were obtained to construct the ceRNA network. By comparing the lncRNAs and mRNAs in two networks, five lncRNAs (SNHG9, LINC02202, UBAC2-AS1, PTCSC3 and myocardial infarction associated transcript (MIAT)) and 32 genes (i.e., such as phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1), protein tyrosine phosphatase receptor type B (PTPRB)) were found to be shared. PPI analysis demonstrated PIK3R1 , forkhead box O1 (FOXO1; a transcription factor) and estrogen receptor 1 (ESR1) were hub genes, which could be regulated by the miRNAs that interacted with the above five lncRNAs, such as LINC02202-miR-136-5p-PIK3R1, LINC02202-miR-381-3p-FOXO1 and MIAT-miR-18a-5p-ESR1. LINC02202 also could directly co-express with PIK3R1. Furthermore, PTPRB was predicted to be modulated by co-expression with LINC01119. Conclusion MIAT, LINC02202 and LINC01119 may be potentially important, new lncRNAs associated with adipogenic differentiation of ASCs. They may be involved in adipogenesis by acting as a ceRNA or co-expressing with their targets.

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Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery

Sharaf

Kleinsasser

Schwenk‐Zieger

et al. 2019

JAMA Facial Plast Surg

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IMPORTANCE Adipose-derived mesenchymal stem cells (ASCs) have been used commonly in regenerative medicine and increasingly for head and neck surgical procedures. Lipoaspiration with centrifugation is purported to be a mild method for the extraction of ASCs used for autologous transplants to restore tissue defects or induce wound healing. The content of ASCs, their paracrine potential, and cellular potential in wound healing have not been explored for this method to our knowledge. OBJECTIVE To evaluate the characteristics of lipoaspirates used in reconstructive head and neck surgical procedures with respect to wound healing. DESIGN, SETTING, AND PARTICIPANTSThis case series study included 15 patients who received autologous fat injections in the head and neck during surgical procedures at a tertiary referral center. The study was performed from October 2017 to November 2018, and data were analyzed from October 2017 to February 2019. MAIN OUTCOMES AND MEASURESExcessive material of lipoaspirates from subcutaneous abdominal fatty tissue was examined. Cellular composition was analyzed using immunohistochemistry (IHC) and flow cytometry, and functionality was assessed through adipose, osteous, and chondral differentiation in vitro. Supernatants were tested for paracrine ASC functions in fibroblast wound-healing assays. Enzyme-linked immunosorbent assay measurement of tumor necrosis factor (TNF), vascular endothelial growth factor (VEGF), stromal-derived factor 1α (SDF-1α), and transforming growth factor β3 (TGF-β3) was performed.RESULTS Among the 15 study patients (8 [53.3%] male; mean [SD] age at the time of surgery, 63.0 [2.8] years), the stromal vascular fraction (mean [SE], 53.3% [4.2%]) represented the largest fraction within the native lipoaspirates. The cultivated cells were positive for CD73 (mean [SE], 99.90% [0.07%]), CD90 (99.40% [0.32%]), and CD105 (88.54% [2.74%]); negative for CD34 (2.70% [0.45%]) and CD45 (1.74% [0.28%]) in flow cytometry; and negative for CD14 (10.56 [2.81] per 300 IHC score) and per 300 IHC score) in IHC staining; they differentiated into osteoblasts, adipocytes, and chondrocytes. The cultivated cells showed high expression of CD44 (mean [SE], 99.78% [0.08%]) and CD273 (82.56% [5.83%]). The supernatants were negative for TNF (not detectable) and SDF-1α (not detectable) and were positive for VEGF (mean [SE], 526.74 [149.84] pg/mL for explant supernatants; 528.26 [131.79] pg/10 6 per day for cell culture supernatants) and TGF-β3 (mean [SE], 22.79 [3.49] pg/mL for explant supernatants; 7.97 [3.15] pg/10 6 per day for cell culture supernatants). Compared with control (25% or 50% mesenchymal stem cell medium), fibroblasts treated with ASC supernatant healed the scratch-induced wound faster (mean [SE]: control, 1.000 [0.160]; explant supernatant, 1.369 [0.070]; and passage 6 supernatant, 1.492 [0.094]). CONCLUSIONS AND RELEVANCEThe cells fulfilled the international accepted criteria for mesenchymal stem cells. The lipoaspirates contained ASCs that had the potential to multidifferentiate w...

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Outcome of Conventional Adipose Tissue Grafting for Contour Deformities of Face and Role of Ex Vivo Expanded Adipose Tissue-Derived Stem Cells in Treatment of Such Deformities

Cited by 18 publications

References 32 publications

Stromal vascular fraction‐enriched platelet‐rich plasma therapy reverses the effects of androgenetic alopecia

Stromal vascular fraction‐enriched platelet‐rich plasma therapy reverses the effects of androgenetic alopecia

Crucial lncRNAs associated with adipocyte differentiation from human adipose-derived stem cells based on co-expression and ceRNA network analyses

Molecular Characterization of Lipoaspirates Used in Regenerative Head and Neck Surgery

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