Outcome of 518 salvage oocyte-cryopreservation cycles performed as a routine procedure in an in vitro fertilization program

Sala, Giovanni Battista La; Nicoli, Alessia; Villani, Maria Teresa; Pescarini, Michela; Gallinelli, Andrea; Blickstein, Isaac

doi:10.1016/j.fertnstert.2006.04.031

Cited by 62 publications

(27 citation statements)

References 9 publications

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“…The survival rate achieved in the present study is similar to that previously observed with the 0.3 M sucrose method [7,15,16,[31][32][33][34][35][36][37] and the 0.2 M sucrose method [17,19,21]. In some of the above studies only oocytes with optimal morphology were cryopreserved [37,38] which is not the case in the present study.…”

Section: Discussionsupporting

confidence: 91%

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

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Previous reports of slow cooling of human mature oocytes have shown a reduced clinical efficiency relative to fresh oocytes. This study reports that equivalent fertilization and implantation rates to those obtained using fresh oocytes and cryopreserved embryos can be achieved with human mature oocytes dehydrated in 1.5 M propanediol and 0.2 M sucrose at 37°C and cryopreserved using slow cooling rates.

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Section: Discussionsupporting

confidence: 91%

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Gook

Edgar

2011

J Assist Reprod Genet

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“…(75)), and human oocyte cryopreservation is still considered an experimental procedure (1). The relatively poor performance of equilibrium freezing has been highlighted in recently published large trials (22)(23)(24). During the past few years, vitrification methods have been applied to cryopreserve human oocytes to determine if improvements can be made (27)(28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

“…Fabbri and colleagues (16) demonstrated a significant improvement in survival when the concentration of sucrose in the freezing medium was increased to 0.3 mol/L; reports of live births using this method or slight variations followed (17)(18)(19)(20)(21). Despite these promising results, very recent reports, some using large cohorts of patients, suggest that the current methods are still sub-optimal (22)(23)(24). It continues to be argued that caution should be taken when applying oocyte cryopreservation in a therapeutic setting (1,10,25).…”

Section: Introductionmentioning

confidence: 99%

Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

Mullen

Mei

et al. 2008

Fertility and Sterility

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Objectives-To determine the permeability of human metaphase II oocytes to ethylene glycol and water in the presence of ethylene glycol, and to use this information to develop a method to vitrify human oocytes.Design-An incomplete randomized block design was used for this study.Setting-A University-affiliated assisted reproductive center.Patients-Women undergoing assisted reproduction in the Center for Reproductive Medicine at Shandong University.Interventions-Oocytes were exposed to 1.0 molar ethylene glycol in a single step, and photographed during subsequent volume excursions.Main outcome measures-A 2-parameter model was employed to estimate the permeability to water and EG.Results-Water permeability ranged from 0.15 to 1.17 µm/(min·atm), and ethylene glycol permeability ranged from 1.5 to 30 µm/min between 7 °C at 36 °C. The activation energies for water and ethylene glycol permeability were 14.42 Kcal/mol and 21.20 Kcal/mol, respectively. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Some of the results from this study were presented at the 62 nd annual meeting of the American Society for Reproductive Medicine, 21-25 October 2006, in New Orleans, LA USA. Conflict of Interest: None. CapsuleHuman metaphase II oocyte permeability to ethylene glycol was determined. A method for vitrification, based upon fundamental principles, was developed from these data. Conclusions-Despite the lower permeability of human MII oocytes to ethylene glycol compared to previously published values for propylene glycol and dimethylsulfoxide, methods to add and remove human oocytes with a vitrifiable concentration of ethylene glycol can be designed which prevent excessive osmotic stress and minimize exposure to high concentrations of this compound. NIH Public Access

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“…Slow-freezing protocols have been mainly studied by Italian researchers. Favourable success rates have been reported, with an increasing number of pregnancies recorded over the years [9][10][11][12][13][14][15][16][17].…”

Section: Techniques For Oocyte Cryopreservationmentioning

confidence: 99%

The current challenges to efficient immature oocyte cryopreservation

Brambillasca

Guglielmo

Coticchio

et al. 2013

J Assist Reprod Genet

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Oocyte cryopreservation represents an important tool for assisted reproductive technology. It offers the opportunity to preserve fertility in women at risk of loss of the ovarian function for various pathologies. It also represents a treatment alternative for couples that cannot benefit from embryo cryopreservation because of moral, religious, or legal constrains. On the other hand, in vitro oocyte maturation has a range of applications. It can be applied in patients with a contraindication to ovarian stimulation to prevent ovarian hyperstimulation syndrome or to eliminate the risk of stimulation of hormone-sensitive tumours in cancer patients. However, while mature oocyte cryopreservation has found widespread application and oocyte in vitro maturation has a place for the treatment of specific clinical conditions, data on the efficiency of freezing of immature or in vitro matured oocytes are poorer. In this review we will focus on the combination of oocyte in vitro maturation with oocyte cryopreservation with particular emphasis on the biological implications of the cryopreservation of immature or in vitro matured oocytes. The two cryopreservation approaches, slow freezing and vitrification, will be discussed in relation to possible cryodamage occurring to subcellular structures of the oocyte and the functional interaction between oocyte and cumulus cells.

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Outcome of 518 salvage oocyte-cryopreservation cycles performed as a routine procedure in an in vitro fertilization program

Cited by 62 publications

References 9 publications

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Implantation rates of embryos generated from slow cooled human oocytes from young women are comparable to those of fresh and frozen embryos from the same age group

Human oocyte vitrification: the permeability of metaphase II oocytes to water and ethylene glycol and the appliance toward vitrification

The current challenges to efficient immature oocyte cryopreservation

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