Outcome of 51 autologous peripheral blood stem cell transplants after uncontrolled-rate freezing (“dump freezing”) using −80°C mechanical freezer

Abstract

BACKGROUND AND OBJECTIVE:Controlled-rate freezing is a complicated, expensive, and time-consuming procedure. Therefore, there is a growing interest in uncontrolled-rate freezing (UCF) with −80°C mechanical freezers for cryopreservation of hematopoietic stem cells. This is a retrospective analysis of efficiency of UCF and outcome of autologous peripheral hematopoietic stem cell (PBSC) transplants at our center from December 2011 to June 2016.MATERIALS AND METHODS:Cryoprotectant solutions used included 5% dimethyl sulfoxide and 5% albumin with 2% hydroxyethyl starch and stored at −80°C mechanical freezer till transplant. Evaluation of cryopreservation was studied by analyzing the variation in cellularity, viability, and CD34+ stem cell dose recovery as well as clinical follow-up with engraftment.RESULTS:A total of 51 patients (23 females and 28 males) underwent autologous PBSC transplantations with a median age of 31 years (range: 3–60 years) for both hematological and nonhematological indications. Mean recovery post by UCF at −80°C mechanical was 92.9% ± 15.5% for nucleated cells, 86.6% ± 15.5% for viability, and 80% ± 21.5% in CD34+ dose. The median day to neutrophil engraftment was 10 (range 5–14 days) and platelets engraftment was 15 (range 8–45 days). The cryopreserved products were stored at −80°C for median 7 days (range 2-41 day) before transplant.DISCUSSION/CONCLUSION:Our analysis shows that PBSC can be successfully cryopreserved with mechanical uncontrolled rate freezing. This is a cheap and simple method to freeze the stem cells for a short period in resource-constrained setting.

The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells

“…Uncontrolled-rate freezing is not a technically or financially demanding method. This method has been most frequently applied to preserve peripheral blood stem cells with consistent results [ 16 , 17 , 18 , 19 ]. Kumar et al studied various uncontrolled-rate freezing protocols and noted that samples directly stored at −80 °C in 10% DMSO in uncontrolled temperature conditions for a month showed the highest proliferation rate [ 20 ].…”

“…The uncontrolled-rate freezing is not a technically or financially demanding technique compared to the previously mentioned methods. Uncontrolled-rate freezing has been most frequently applied for the preservation of peripheral blood stem [ 16 , 17 , 18 , 19 ]. Only a few published studies have described the uncontrolled-rate freezing during subsequent storage of stem cells at −80 ° C [ 20 , 21 ].…”

Real-World Evidence Data on Adverse Reactions to Infusion of Thawed Hematopoietic Progenitor Cells: Retrospective Analysis from a Single Center in India

“…To the best of the author's knowledge, this is the first such study from India. There are several previous studies from India [9][10][11][12] that have reported long-term complications and transplant outcomes after infusion of cryopreserved HPC. Only one of these studies by Setia et al 12 briefly mentions ASs during the infusion.…”

“…There are several previous studies from India [9][10][11][12] that have reported long-term complications and transplant outcomes after infusion of cryopreserved HPC. Only one of these studies by Setia et al 12 briefly mentions ASs during the infusion. However, studying the ASs during the infusion of thawed HPC was not an objective of any of these studies.…”

Cryostorage of peripheral blood hematopoietic stem cells in transplantology: current status and prospects

“…Поэтому активно растет интерес к процессу замораживания с неконтролируемой скоростью с использованием стандартных морозильных камер, который можно легко адаптировать в условиях ограниченных ресурсов. Тем более, что консервация с неконтролируемой скоростью не приводит к существенному снижению эффективности трансплантации ГСК периферической крови [22]. С целью изучения и проверки более простых и экономичных способов заморозки ГСК, Calvet L, et al (2012) провели клиническое исследование 342 аутотрансплантантов после хранения в течение 3, 6 и 12 мес.…”