Outbreaks of Vesicular Stomatitis in Brazil caused by a distinct lineage of Alagoas vesiculovirus

Oliveira, Anapolino Macedo de; Laguardia-Nascimento, Mateus; Sales, Mariana Lázaro; Júnior, Anselmo Rivetti Vasconcelos; Camargos, Marcelo Fernandes; Melo, Cristiano Barros de; Júnior, Antônio Augusto Fonseca

doi:10.1007/s42770-021-00537-9

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…Brazil, in a broader way, considering the OVS, has been investing in training, data analysis and staff training with a scientific basis, including themes related to the inspection of products of animal origin (Rodrigues et al, 2017(Rodrigues et al, , 2018(Rodrigues et al, , 2021, international agricultural surveillance (Melo et al, 2014a(Melo et al, , 2014b(Melo et al, , 2015Sá et al, 2018), agricultural defence (Moraes et al, 2017;Oliveira et al, 2021) and diagnosis and official laboratory structure (Dias et al, 2014;Oliveira et al, 2018Oliveira et al, , 2021. This shows that the country has been preparing for the new sanitary challenges in livestock and in this sense, the DF continues to adapt to international requirements, as demonstrated in this paper, especially considering the stage Brazil is at in the National Programme for the Eradication of FMD, in which the intention is to gradually remove vaccination against FMD from cattle and buffalo herds in Brazilian states, in accordance with the proposal approved by the OIE (Brasil, 2019).…”

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confidence: 99%

Challenges to and advancements of the official veterinary service of the Federal District, Brazil

Taques¹,

Coelho

Barbosa-Júnior

et al. 2021

Braz. J. Vet. Med.

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Animal diseases can be both a precursor to social instability and a result of social instability. For disease outbreaks to be prevented or even quickly controlled, an efficient and well-structured veterinary service is fundamental. The monitoring of the efficiency of an official veterinary service (OVS) by official audits is a sine qua non condition for the progress of an efficient way to control and eradicate diseases. In this sense, the present work aims to study the advances and challenges of the OVS of the Federal District (DF), Brazil, from a study of the scores obtained by the evaluation of the Programme of Quality Evaluation and Improvement of Official Veterinary Services which is based on the World Organisation for Animal Health -Performance of Veterinary Services Pathway (Quali-SV/OIE-PVS), which was used to officially assess the quality and improvement of the OVS of the DF. In Brazil, the official evaluation was conducted by the Coordination of Evaluation and Improvement of Veterinary Services, Ministry of Agriculture, Livestock and Food Supply, considering manpower, infrastructure and financial resources. Five possible scoring levels were audited: authority, technical and operational capacity, interaction with stakeholders and access to markets. These components were described and assessed as critical competencies in the OVS assessment. Strengths and weaknesses observed during the audit were listed, and levels of OVS progress were rated and assessed. Despite the predominance of strengths in the OVS audit, the weaknesses detected require urgent corrective action, especially with regard to the autonomy of the OVS.

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Section: /11mentioning

confidence: 99%

Challenges to and advancements of the official veterinary service of the Federal District, Brazil

Taques¹,

Coelho

Barbosa-Júnior

et al. 2021

Braz. J. Vet. Med.

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“…As in studies conducted in the USA and Mexico, results of genomic analysis between the first Brazilian VSAV isolate and isolates in the last 12 years showed that these virus samples clustered into three distinct groups: group A formed exclusively by VSAV isolated in 1964, group B formed by viruses from outbreaks in the northeast region, and a third group, named C, formed by isolates from the states of Minas Gerais, Bahia, Goiás, and Tocantins. The genetic distances of protein P between the current groups, C and B, concerning group A is considerably high, being above 49% [8,9] (de Oliveira et al, 2021, Fonseca Jr. et al, 2022. This work aims to compare virus growth in cell cultures of three distinct VASV strains isolated in Brazil using quantification of viral RNA by digital droplet PCR.…”

Section: Introductionmentioning

confidence: 99%

Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR

et al. 2023

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Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study.

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Outbreaks of Vesicular Stomatitis in Brazil caused by a distinct lineage of Alagoas vesiculovirus

Cited by 2 publications

References 15 publications

Challenges to and advancements of the official veterinary service of the Federal District, Brazil

Challenges to and advancements of the official veterinary service of the Federal District, Brazil

Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR

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