Outbreak of Salmonella in Poultry of Bangladesh and possible remedy

Hoque,; Mohiuddin, Rasha; Khan, Mohammad Imran; Hannan, Mst; Alam, Mahtab

doi:10.5455/jabet.2019.d30

Cited by 13 publications

(16 citation statements)

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“…In the present study, detection of the invA gene in 10 tested Salmonella isolates indicated their pathogenic nature. Antimicrobial resistance in livestock associated Salmonella spp., including fluoroquionolone and colistin resistant Salmonella spp., is an emerging global public health threat [ 4 ]. In the present study, antimicrobial susceptibility results showed that the majority of the tested Salmonella isolates were found resistant to colistin, ciprofloxacin and tigecycline.…”

Section: Discussionmentioning

confidence: 99%

“…Salmonellosis remains a persistent threat to both human and animal health [ 2 , 3 ]. Although vaccination and good hygienic practices are among the most effective measures to prevent salmonellosis [ 4 ], antibiotics are extensively used either as growth promoters [ 5 , 6 ], prophylactic agents, or therapeutics in the poultry industry in Bangladesh [ 7 ]. The widespread misuse and overuse of antimicrobial agents in poultry settings contribute to the emergence and the development of antimicrobial resistance in livestock pathogens such as Salmonella [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Uddin

Hossain

Hasan

et al. 2021

Animals

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Colistin (polymyxin E) is widely used in animal and human medicine and is increasingly used as one of the last-resort antibiotics against Gram-negative bacilli. Due to the increased use of colistin in treating infections caused by multidrug-resistant Gram-negative bacteria, resistance to this antibiotic ought to be monitored. The study was undertaken to elucidate the molecular mechanisms, genetic relationships and phenotype correlations of colistin-resistant isolates. Here, we report the detection of the mcr-1 gene in chicken-associated Salmonella isolates in Bangladesh and its in-silico functional analysis. Out of 100 samples, 82 Salmonella spp. were isolated from chicken specimens (liver, intestine). Phenotypic disc diffusion and minimum inhibitory concentration (MIC) assay using different antimicrobial agents were performed. Salmonella isolates were characterized using PCR methods targeting genus-specific invA and mcr-1 genes with validation for the functional analysis. The majority of the tested Salmonella isolates were found resistant to colistin (92.68%), ciprofloxacin (73.17%), tigecycline (62.20%) and trimethoprim/sulfamethoxazole (60.98%). When screened using PCR, five out of ten Salmonella isolates were found to carry the mcr-1 gene. One isolate was confirmed for Salmonella enterica subsp. enterica serovar Enteritidis, and other four isolates were confirmed for Salmonella enterica subsp. enterica serovar Typhimurium. Sequencing and phylogenetic analysis revealed a divergent evolutionary relationship between the catalytic domain of Neisseria meningitidis lipooligosaccharide phosphoethanolamine transferase A (LptA) and MCR proteins, rendering them resistant to colistin. Three-dimensional homology structural analysis of MCR-1 proteins and molecular docking interactions suggested that MCR-1 and LptA share a similar substrate binding cavity, which could be validated for the functional analysis. The comprehensive molecular and in-silico analyses of the colistin resistance mcr-1 gene of Salmonella spp. of chicken origin in the present study highlight the importance of continued monitoring and surveillance for antimicrobial resistance among pathogens in food chain animals.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Uddin

Hossain

Hasan

et al. 2021

Animals

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“…Eggs and meat from poultry are indispensable protein sources in peoples meals in Bangladesh [ 1 ]. However, the advancement in the poultry production is often interrupted by the overwhelming occurrence of infectious diseases in low resource settings of developing countries such as Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

“…A high incidence (53.5%) of Salmonella infections in adult layer chickens has been reported for the low resource settings in this country [ 22 ]. Moreover, a number of investigations have confirmed wide-scale occurrence of antimicrobial resistance in Salmonella spp., with variable incidence, between 20% and 100%, in poultry animals and associated environmental samples in Bangladesh [ 1 , 23 , 24 , 25 , 26 ]. Among different types of salmonellosis in poultry, Salmonella Gallinarum can cause more than 70% infection in layer chicken [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Salmonella Gallinarum in Small-Scale Commercial Layer Flocks: Occurrence, Molecular Diversity and Antibiogram

Haque

Akter

Islam

et al. 2021

Veterinary Sciences

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Salmonella Gallinarum is one of the most important bacterial pathogens associated with diminished egg production in poultry. The aim of this study was to understand the occurrence, molecular traits and antimicrobial resistance patterns of Salmonella Gallinarum strains isolated from small-scale commercial layer flocks with low level biosecurity standards in Bangladesh. A total of 765 samples, including cloacal swabs (535), visceral organs (50), and droppings (180), were collected from chickens of 12 layer flocks in 11 districts. Salmonella Gallinarum was isolated and characterized through culture-based method, followed by biochemical tests, sero-grouping, PCR assays, sequencing, and antibiogram. The identity of biochemically detected isolates of Salmonella Gallinarum was confirmed via genus-specific 16S rRNA gene based PCR, followed by invA and spvC genes based PCR assays. Occurrence of Salmonella Gallinarum was detected in overall 25.75% (197/765) samples, with a significantly (p < 0.05) higher incidence in visceral organs (42%) in comparison to cloacal swab (24%) and droppings (26%). Sequencing and subsequent phylogenetic analysis of invA and spvC genes in representative strains of Salmonella Gallinarum revealed a close genetic lineage, with a sequence similarity of 98.05–99.21% and 97.51–99.45%, respectively, to previously published sequences of the corresponding genes from the same serogroup strains. Remarkably, 66.5% (131/197) of the isolated strains of Salmonella Gallinarum were found to be resistant to 3 to 6 antimicrobial agents, and interpreted as multidrug resistant (MDR). The findings of this study underscore an inherent need of appropriate control measures to curb the widespread incidence of MDR Salmonella Gallinarum in small-scale commercial layer flocks, thereby, facilitating enhanced egg production and further support to the food security and safety in low resource settings.

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“…While various studies have been conducted in Bangladesh to investigate the health concerns associated with LBMs [ 4 , 7 , 10 ], there is a dearth of knowledge regarding the regulatory environment and problems confronting LBMs. As a result, this paper examines Bangladesh’s existing policies and regulations governing LBMs, as well as their implementation.…”

Section: Introductionmentioning

confidence: 99%

Live bird market in Bangladesh: Regulatory systems and operations

Irin¹,

Dilshad²,

Sattar³

et al. 2021

J Adv Vet Anim Res

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Objective: In developing countries, such as Bangladesh, the live bird market (LBM) is a vital location for the trading of live poultry. The study was carried out in nine LBMs located around Bangladesh to ascertain the present regulations and procedures governing their operation. Additionally, the responsibilities and levels of engagement of the stakeholders were determined. Materials and Methods: The data were gathered through the use of a semi-structured interview guide. Thematic analysis was used to code the interview transcripts iteratively. Results: The findings indicated that the government was directly and indirectly involved in the leasing process of the markets. A market in this country is divided into numerous sectors, including LBM, fish market, vegetable market, and grocery stores. A market’s hygienic condition is highly dependent on market authority’s decisions. In some markets, market officials conducted routine sanitary inspections. Veterinarians played a little role in the inspection procedure. Conclusion: There is no adequate, functional monitoring system to ensure that LBMs adhere to cleanliness and adequate and functional biosecurity. Biosecurity enhancements, effective cleaning programs, and regular monitoring by relevant authorities are critical for LBMs in Bangladesh.

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Outbreak of Salmonella in Poultry of Bangladesh and possible remedy

Cited by 13 publications

References 0 publications

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Salmonella Gallinarum in Small-Scale Commercial Layer Flocks: Occurrence, Molecular Diversity and Antibiogram

Live bird market in Bangladesh: Regulatory systems and operations

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