Outbreak of nosocomial infections with two different MRSA-strains involved: significance of genomic DNA fragment patterns in strains otherwise difficult to type

Cuny, Cécile; Schassan, H. H.; Witte, Wolfgang

doi:10.1017/s0950268800056673

Cited by 14 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,8,32-37 Molecular typing methods were used to confirm these findings in 15 studies. 24,33,35,[38][39][40][41][42][43][44][45][46][47][48][49] The preventability of MRSA spread was demonstrated in this study by the low number of nosocomial infections or colonizations due to MRSA, despite repeated reintroductions between 1986 and 1990 ( Figure 1). Overall, only 10% of nosocomial staphylococcal infections were due to MRSA during the 8-year study, despite admission of 133 colonized patients during this period.…”

Section: December 1995supporting

confidence: 50%

Control of Methicillin-Resistant Staphylococcus aureus at a University Hospital: One Decade Later

Jernigan¹,

Clemence²,

Stott³

et al. 1995

Infection Control and Hospital Epidemiology

126

View full text Add to dashboard Cite

An increase in the incidence of nosocomial MRSA infection was associated with an increased frequency of transfer of colonized patients from nursing homes and other hospitals. The lack of correlation between rates of MRSA and MSSA infections suggested that MRSA infections significantly increased the overall rate of staphylococcal infection. Screening cultures of transfer patients from facilities with a high prevalence of MRSA may offer significant benefit by preventing nosocomial infections and reducing patient days spent in isolation.

show abstract

Section: December 1995supporting

confidence: 50%

Control of Methicillin-Resistant Staphylococcus aureus at a University Hospital: One Decade Later

Jernigan¹,

Clemence²,

Stott³

et al. 1995

Infection Control and Hospital Epidemiology

126

View full text Add to dashboard Cite

show abstract

“…The B‐type strain has been isolated from hospitals throughout Poland, whereas the A type has only been isolated in Warsaw, but this may be explained by the failure of laboratories outside Warsaw to recognize this strain [19]. It is important to note that the A and B strains can be found together in a single hospital, showing that the coexistence of different MRSA clones is feasible [28].…”

Section: Discussionmentioning

confidence: 99%

Two clones of methicillin-resistant Staphylococcus aureus in Poland

Trzciński¹,

Leeuwen

Belkum³

et al. 1997

Clinical Microbiology and Infection

View full text Add to dashboard Cite

OBJECTIVE: To evaluate relatedness among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in Poland. METHODS: Ninety-three MRSA hospital isolates were collected from different regions in Poland from 1990 to 1992. Strains were analyzed with respect to heterogeneity of methicillin resistance, phage types, resistance patterns, crystal violet staining, chromosomal DNA SmaI restriction patterns by PFGE, ERIC1 and ERIC2 AP-PCR types and DNA repeat polymorphism within the protein A gene. Resistance to methicillin was confirmed by the detection of the mecA gene by PCR. RESULTS: The combined results of typing methods demonstrate that all MRSA strains analyzed could be easily divided into two distinct clones (clonally related strains). The first consisted of strains with clear heterogeneous expression of resistance to methicillin (34 isolates) and the second showed more homogeneous resistance (59 isolates). In this study the best method for epidemiologic analysis of MRSA was found to be PFGE. A good correlation between the epidemic behavior of MRSA and a high number of repetitive DNA units within the protein A gene was observed. CONCLUSIONS: Results show that in Poland two distinct clones of epidemic MRSA have circulated in the past, easily discriminated by pheno- and genotyping methods, and both could be found together in a single hospital.

show abstract

“…Genotyping of S. aureus was performed by SmaI macrorestriction analysis using tar916-shida PCR and pulsed-field gel electrophoresis. 4,5 As shown in Fig. 1, the SmaI-macrorestriction pattern corresponded to that of the so called ÔBarnim epidemic MRSAÕ.…”

Section: Reportmentioning

confidence: 64%

Methicillin-resistant Staphylococcus aureus (MRSA) sepsis following mesh graft transplantation to treat chronic leg ulcer

Haas

Kimmritz

Witte

et al. 2002

Clinical and Experimental Dermatology

Self Cite

View full text Add to dashboard Cite

Infections with methicillin-resistant strains of Staphylococcus aureus (MRSA) from colonized leg ulcers are rare. We describe a case of MRSA sepsis following mesh graft transplantation to treat a chronic leg ulcer. MRSA were isolated from blood and midstream urine and typed by their antimicrobial sensitivity, phage and SmaI-macrorestriction patterns. A strain found during sepsis was identical to an epidemic MRSA related to epidemic MRSA 15 from Great Britain. Control swabs to detect MRSA should be implemented in epidemic areas of MRSA before grafting chronic ulcers.

show abstract

Outbreak of nosocomial infections with two different MRSA-strains involved: significance of genomic DNA fragment patterns in strains otherwise difficult to type

Cited by 14 publications

References 15 publications

Control of Methicillin-Resistant Staphylococcus aureus at a University Hospital: One Decade Later

Control of Methicillin-Resistant Staphylococcus aureus at a University Hospital: One Decade Later

Two clones of methicillin-resistant Staphylococcus aureus in Poland

Methicillin-resistant Staphylococcus aureus (MRSA) sepsis following mesh graft transplantation to treat chronic leg ulcer

Contact Info

Product

Resources

About