Outbreak of Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae with high qnr prevalence in a Chinese hospital

Zhang, Rong; Wang, Xuanding; Cai, Jiachang; Hong, Zhou; Lv, H.-J.; Hu, Qing; Chen, Gongxiang

doi:10.1099/jmm.0.015826-0

Cited by 34 publications

(31 citation statements)

References 30 publications

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“…In China, KPC-2 was first detected in 2007 from a CR-KP isolate (Queenan and Bush 2007;Nordmann et al 2011;Tzouvelekis et al 2012). At present, KPC-2 is thought to be a predominant carbapenemase in Enterobacteriaceae in our country (Chen et al 2011;Qi et al 2011;Zhang et al 2011). However, in this study, the prevalence rate of KPC-2 in CR-KPs from our hospital is only 17.5% (7/40), which is much lower than rates in other studies in China (Chen et al 2011;Qi et al 2011;Zhang et al 2011).…”

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confidence: 82%

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High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Zhao

et al. 2014

Can. J. Microbiol.

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The aim of this study was to characterize the carbapenemases in carbapenem-resistant Klebsiella pneumoniae (CR-KP) from a Chinese teaching hospital. A total of 40 CR-KPs were screened for the presence of carbapenemases. Minimum inhibitory concentrations were determined by agar dilution. The modified Hodge test was used for the detection of carbapenemase production. Carbapenemase, extended-spectrum β-lactamase, and AmpC genes were detected using polymerase chain reaction (PCR) and sequencing. A conjugation test was performed using a broth culture mating method, transferred plasmids were typed by PCR-based replicon typing, and clonal relatedness was investigated by enterobacterial repetitive intergenic consensus sequences PCR (ERIC-PCR) and multilocus sequence typing (MLST). The results revealed that modified Hodge test was positive for 28 CR-KPs, and CR-KPs exhibited high resistance rates against various antibiotics, except colistin (5.0%) and tigecycline (22.5%). ERIC and MLST profiles showed no clonal outbreak. PCR demonstrated a high prevalence rate (55.0%, 22/40) of metallo-β-lactamases (MBLs) in CR-KPs. IMP-4, IMP-8, NDM-1, and KPC-2 were identified in 14 (35.0%), 7 (17.5%), 2 (5.0%), and 7 (17.5%) isolates, respectively. Notably, 2 CR-KPs coproduced 2 carbapenemases simultaneously (IMP-8/NDM-1 and IMP-4/KPC-2). In vitro transfer of carbapenem resistance was successful for 11 MBL-producing CR-KPs. The extended spectrum β-lactamase genes were detected in 30 (75.0%) of these CR-KPs. To the best of our knowledge, this is the first report focusing on carbapenem resistance in K. pneumoniae due to metalloenzymes in China. Screening and surveillance of MBLs in Enterobacteriaceae is urgently needed in this region to control and prevent the spread of these resistance determinants.

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confidence: 82%

“…In China, CR-KPs have disseminated widely in recent years, and most of them are typically attributed to the production of KPCs (Chen et al 2011;Qi et al 2011;Zhang et al 2011). In this study, we performed a local surveillance study of CR-KPs to investigate the prevalence of different carbapenemase resistance genes in a Chinese teaching hospital.…”

mentioning

confidence: 99%

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Zhao

et al. 2014

Can. J. Microbiol.

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“…Using specific primers and sequencing (12), a qnrB19 gene was detected in the parental strain. These results were similar to those of previous reports which demonstrated that KPC-producing isolates are resistant not only to all ␤-lactam antibiotics, including carbapenems, but also to some non-␤-lactam antibiotics, such as fluoroquinolones (13,14).…”

supporting

confidence: 82%

Emergence of KPC-2-Producing Salmonella enterica Serotype Schwarzengrund in Argentina

Jure

Duprilot

Musa

et al. 2014

Antimicrob Agents Chemother

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“…5 Though KPC played a major role in high-level resistance to carbapenems and other (8-lactams, several other ^-lactamases, such as TEM, SHV, and CTX-M enzymes, also might have existed in K. pneumoniae simultaneously. 6 ' 7 However, in our study, only WaT EM and bla^c were discovered in zjm002. Considering that the TEM enzyme contributed nothing directly to carbapenem resistance, the KPC enzyme might be the result of it.…”

Section: Novel Kpc Variant From a Carbapenemresistant Strain Of Klebscontrasting

confidence: 61%

Novel KPC Variant from a Carbapenem-Resistant Strain of Klebsiella pneumoniae in a Chinese Hospital

Zhu

Jiang

Mi³

et al. 2011

Infect. Control Hosp. Epidemiol.

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Outbreak of Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae with high qnr prevalence in a Chinese hospital

Cited by 34 publications

References 30 publications

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Emergence of KPC-2-Producing Salmonella enterica Serotype Schwarzengrund in Argentina

Novel KPC Variant from a Carbapenem-Resistant Strain of Klebsiella pneumoniae in a Chinese Hospital

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