Outbreak of Febrile Respiratory Illness Associated with Adenovirus 11a Infection in a Singapore Military Training Camp

Kajon, Adriana E.; Dickson, Laura M.; Metzgar, David; Houng, Huo-Shu H.; Lee, Vernon; Tan, Boon Huan

doi:10.1128/jcm.01928-09

Cited by 89 publications

(97 citation statements)

References 29 publications

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“…For example, our qPCR assay amplifies both HAdV genome types 11p (strain Slobitski) and 11a (also designated HAdV-11/H14 [38] and HAdV-55 [39]) with equal efficiency (data not shown). The former is rarely isolated and more often associated with acute hemorrhagic cystitis and immunocompromised host disease (40), whereas the latter, which emerged as a respiratory pathogen causing outbreaks of ARI (16,41,42,43), was found to possess the HAdV-14 fiber gene, likely acquired through recombination (12). In these cases, partial hexon and fiber genes or full-genome sequencing would be recommended to more fully characterize these viruses (26).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Real-Time PCR Assay Panel for Detection and Type-Specific Identification of Epidemic Respiratory Human Adenoviruses

Trujillo-Lopez²,

Lott³

et al. 2013

J Clin Microbiol

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bOutbreaks of human adenovirus (HAdV) acute respiratory illness (ARI) have been well documented among civilians and unvaccinated military recruits. Among the 7 recognized HAdV species (A to G), species B (particularly serotypes 3, 7, 11, 14, and 21) and E (serotype 4) have more often been associated with epidemic ARI. Rapid detection and type-specific identification of these viruses would enhance outbreak response and help guide prevention and control measures. To this end, we developed type-specific real-time quantitative PCR (qPCR) assays for HAdV types 3, 4, 7, 11, 14, 16, and 21 targeting the HAdV hexon gene. All typespecific qPCR assays reproducibly detected as few as 10 copies/reaction of quantified hexon recombinant plasmids with a linear dynamic range of 8 log units (10 1 to 10 8 copies); in contrast, a generic qPCR assay that detects all HAdV types run concurrently detected between 10 and 100 copies/reaction, depending on the virus type. No nonspecific amplifications were observed with concentrated nucleic acid from 51 HAdV prototype strains or other common respiratory pathogens. All members of a panel of 137 previously typed HAdV field isolates and positive clinical specimens were correctly characterized by the type-specific qPCR assays; two different HAdV types were detected in three of the clinical specimens and confirmed by amplicon sequencing. The qPCR assays permit sensitive, specific, and quantitative detection and identification of seven clinically important respiratory HAdVs and should provide a convenient adjunct to classical typing methods for a rapid response to HAdV outbreaks.

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Section: Discussionmentioning

confidence: 99%

Quantitative Real-Time PCR Assay Panel for Detection and Type-Specific Identification of Epidemic Respiratory Human Adenoviruses

Trujillo-Lopez²,

Lott³

et al. 2013

J Clin Microbiol

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“…All patients were young, previously healthy soldiers. Although several studies have described the clinical features of adenovirus infection among military personnel (7)(8)(9)(10)(11)(12)(13), few studies have compared the clinical characteristics of pneumonic and non-pneumonic adenovirus cases or evaluated the risk factors associated with military training courses. Furthermore, few reports have provided detailed descriptions of the radiological features of adenovirus infections by means such as thinsection computed tomography (CT) (7,9).…”

Section: Introductionmentioning

confidence: 99%

Outbreak of Febrile Respiratory Illness Caused by Adenovirus at a South Korean Military Training Facility: Clinical and Radiological Characteristics of Adenovirus Pneumonia

Hwang

Park²,

Yong³

et al. 2013

Jpn J Infect Dis

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SUMMARY: Adenovirus often causes respiratory disease outbreaks in military training soldiers. Compared with adenovirus pneumonia in young military adults, symptoms arising from febrile respiratory illness (FRI) caused by adenovirus have not been previously evaluated in Korean soldiers. We conducted an event-based outbreak investigation involving 712 male soldiers aged 19 to 21 years from March 14 to 30, 2012 to evaluate the epidemiological and clinical characteristics of patients with pneumonia and FRI caused by adenovirus. We described the laboratory and radiological characteristics of patients with adenovirus pneumonia. Among these, 407 cases of FRI and 15 cases of pneumonia were identified through active surveillance (attack rate of FRI, 57.16z; attack rate of pneumonia, 2.11z). Fire training and march training may present environmental risk factors for adenovirus-associated outbreaks. Most symptoms were mild. The most frequent symptom in patients with pneumonia and FRI was cough. Patients with pneumonia were associated with an increased incidence of dizziness (crude odds ratio [cOR], 9.65; 95z confidence interval [CI], 2.38-37.15) and a decreased incidence of rhinorrhea (cOR, 0.15; 95z CI, 0.04-0.53) during adenovirus-associated outbreaks. Differential leukocyte count revealed high monocytes, low lymphocytes, and low eosinophils, and chest computed tomography revealed a consolidation pattern and right lobar pneumonia. These findings warrant a high level of suspicion for adenovirus pneumonia.

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“…Recombination of HAdV genomes plays a role in pathogen genesis (3,5,6,8,9,10,12,13). This presentation of the first HAdV-B14 isolate in China facilitates an understanding of the evolution of HAdV-B55 and provides awareness of possible recombination events between wild-type viruses and adenoviral gene delivery vectors, with additional information for the development of adenovirus vaccines.…”

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confidence: 99%

Genome Sequence of the First Human Adenovirus Type 14 Isolated in China

Zhang

Seto

Zhao

et al. 2012

J Virol

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Emergent pathogens may be examined rapidly at high resolution on a molecular level using genomics, allowing an understanding of their evolution. China is a unique environment for studying pathogens, having a large, dense, and generally closed population. Human adenovirus type 14 (HAdV-14) was originally identified as an acute respiratory disease (ARD) pathogen in The Netherlands (1955), with a second isolation in England (1957). Since then, few reports of this virus appeared until an ARD pathogen with a similar genome caused multiple outbreaks in the United States (2006 to 2009). This report presents the first genome of HAdV-B14 isolated in China (2010). As China experienced two recent outbreaks of an emergent ARD pathogen, HAdV-B55, containing much of the HAdV-B14 genome, the availability of this HAdV-B14 sequence will facilitate studies of the epidemiology of these pathogens, as well as provide a foundation for studying adenovirus evolution and the genesis of emergent pathogens. These observations may be invaluable in predicting possible recombination between wild-type viruses and adenoviral gene delivery vectors, including adenovirus vaccines.

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Outbreak of Febrile Respiratory Illness Associated with Adenovirus 11a Infection in a Singapore Military Training Camp

Cited by 89 publications

References 29 publications

Quantitative Real-Time PCR Assay Panel for Detection and Type-Specific Identification of Epidemic Respiratory Human Adenoviruses

Quantitative Real-Time PCR Assay Panel for Detection and Type-Specific Identification of Epidemic Respiratory Human Adenoviruses

Outbreak of Febrile Respiratory Illness Caused by Adenovirus at a South Korean Military Training Facility: Clinical and Radiological Characteristics of Adenovirus Pneumonia

Genome Sequence of the First Human Adenovirus Type 14 Isolated in China

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