Outbreak of a susceptible strain of Acinetobacter species 13 (sensu Tjernberg and Ursing) in an adult neurosurgical intensive care unit

Dessel, Helke van; Kamp-Hopmans, T. E. M.; Fluit, Ad C.; Brisse, Sylvain; Smet, Anne Marie G. A. de; Dijkshoorn, Lenie; Troelstra, Annet; Verhoef, J.; Mascini, Ellen M.

doi:10.1053/jhin.2002.1212

Cited by 48 publications

(27 citation statements)

References 28 publications

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“…The reported incidence of bacteremia after diagnostic upper gastrointestinal endoscopy is about 2-5% (18,19) (22,23). Although a common environmental source for this pathogen was not found in the present case, the most possible source was the endoscope which was contaminated by the hands of staff or the hospital environment (20,22). To deal with the patients with a high risk for endocarditis, it is more important to notice hand washing and disinfection of the endoscope to prevent this event.…”

Section: F I G U R E 2 T H E Wh O L E C L I N I C a L C O U R S Ementioning

confidence: 58%

Nosocomial Acinetobacter Genomic Species 13 TU Endocarditis Following an Endoscopic Procedure

Yu-Hsien

Chen

et al. 2008

Intern. Med.

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Section: F I G U R E 2 T H E Wh O L E C L I N I C a L C O U R S Ementioning

confidence: 58%

Nosocomial Acinetobacter Genomic Species 13 TU Endocarditis Following an Endoscopic Procedure

Yu-Hsien

Chen

et al. 2008

Intern. Med.

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“…Interestingly, the ITS length (607 bp) of these three genomic species 13TU strains was also 607 bp, identical to that of strain 74510 (Table 1). In addition, the ITS sequences of these genomic species 13TU strains 74510, v104-2, 00574, and DR25612/96 appeared to be more closely related to the A. baumannii (genomic species 2) strain BCRC 10591 T than to the 13TU reference strain BCRC 15417 (0.9538 to 0.9588 versus 0.9171 identity) (Table 1), which reinforces the notion that genetic similarity among A. baumannii and genomic species 13TU is high (3) and perhaps suggests that BCRC 15417 may not be as representative for genomic species 13TU.Acinetobacter genomic species 13TU is the second most clinically relevant species in the Acb complex (3,5,6). Therefore, we believe that it is important to extend the number of Acinetobacter genomic species 13TU ITS sequences in the ITS sequence database for more-accurate genomic species identification.…”

mentioning

confidence: 58%

“…Acinetobacter genomic species 13TU is the second most clinically relevant species in the Acb complex (3,5,6). Therefore, we believe that it is important to extend the number of Acinetobacter genomic species 13TU ITS sequences in the ITS sequence database for more-accurate genomic species identification.…”

mentioning

confidence: 99%

Identification of Acinetobacter Genomic Species 13TU by Sequence Analysis of the 16S-23S rRNA Gene Spacer Region

et al. 2009

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The genus Acinetobacter currently contains up to 32 described named and unnamed (genomic) species (3). Acinetobacter baumannii, genomic species 3, and 13TU, three of the most clinically relevant species, are genetically and phenotypically very similar to an environmental species, Acinetobacter calcoaceticus, and are therefore grouped together into the socalled A. calcoaceticus-A. baumannii (Acb) complex (3). Because phenotypic identification of Acinetobacter isolates to the species level has proven to be insufficient, several genotypic methods have been developed for genomic species identification (1, 3, 4).In the last 3 years, our laboratory has routinely used the 16S-23S rRNA gene intergenic spacer (ITS) sequence-based method described by Chang et al.(1) to delineate isolates belonging to the Acb complex to the genomic species level. We found the procedure accurate and easy to perform. However, we have recently encountered a discrepancy during the characterization of Acinetobacter strain 74510 isolated originally from a clinical source at the Prince of Wales Hospital, Hong Kong, in 1995 (2). Strain 74510 was previously identified as genomic species 13TU by restriction analysis of amplified ribosomal DNA (2, 4). However, examination of the single PCR amplicon of the ITS region of strain 74510 (GenBank accession no. FJ360743) revealed an ITS sequence length of 607 bp, as opposed to the 615 bp reported by Chang et al. for the genomic species 13TU reference strain BCRC 15417 (1). On the other hand, DNA sequence analysis of this 607-bp ITS of strain 74510 showed an identity of 0.9561 to that of strain BCRC 15417 (Table 1). Upon further analysis of nucleotide sequences in GenBank, the strain 74510 ITS sequence was also found to share increasing identity (0.9950 to 0.9967) with three other genomic species 13TU strains, namely, v104-2, 00574, and DR25612/96 (Table 1). Interestingly, the ITS length (607 bp) of these three genomic species 13TU strains was also 607 bp, identical to that of strain 74510 (Table 1). In addition, the ITS sequences of these genomic species 13TU strains 74510, v104-2, 00574, and DR25612/96 appeared to be more closely related to the A. baumannii (genomic species 2) strain BCRC 10591 T than to the 13TU reference strain BCRC 15417 (0.9538 to 0.9588 versus 0.9171 identity) (Table 1), which reinforces the notion that genetic similarity among A. baumannii and genomic species 13TU is high (3) and perhaps suggests that BCRC 15417 may not be as representative for genomic species 13TU.Acinetobacter genomic species 13TU is the second most clinically relevant species in the Acb complex (3,5,6). Therefore, we believe that it is important to extend the number of Acinetobacter genomic species 13TU ITS sequences in the ITS sequence database for more-accurate genomic species identification. Also, we would suggest that the reference ITS sequence length for genomic species 13TU be 607 bp long and that strain 74510 could be considered as the "type" strain for genomic species 13TU.

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“…This genomic species was well discriminated from A. baumannii and gen. sp. 3 and was also associated with nosocomial outbreaks (Dijkshoorn & van der Toorn, 1992;Seifert et al, 1995;van Dessel et al, 2002). A. lwoffii, which usually does not play a role in hospital-acquired infections, was present in only one specimen.…”

Section: Discussionmentioning

confidence: 99%

Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species

et al. 2009

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Bacteria belonging to the genus Acinetobacter are ubiquitous in soil and water. Only a few species, including Acinetobacter baumannii, and the unnamed Acinetobacter genomic species (gen. sp.) 3 and 13TU, which together with the soil organism Acinetobacter calcoaceticus are combined in the A. calcoaceticus-A. baumannii (Acb) complex, have been recognized as important nosocomial infectious agents. The ecology, epidemiology and pathology of most species are not yet well established. Lack of practical and accurate methods limits routine identification of clinical isolates and thus hampers precise identification of those of the Acb complex and other Acinetobacter species of possible clinical significance. We previously identified a 350 bp highly variable zone on the rpoB gene which appeared to be a promising target for rapid molecular identification. In the present study, we validated this method for accuracy on a collection of reference strains belonging to A. calcoaceticus (5 strains), Acinetobacter gen. sp. 3 (29 strains), A. gen. sp. 13TU (18 strains), A. baumannii (30 strains) and one strain each of A. radioresistens, A. gen. sp. 15TU, A. gen. sp. 10, A. gen. sp. 11, A. gen. sp. 'between 1 and 3' and A. gen. sp. 14TU513BJ. This represents the largest analysis to date that compares a large number of well-identified strains of the Acb complex to assess the intra-and interspecies variation within this complex. All were correctly identified with 98.9-100 % intraspecies relatedness based on partial rpoB sequence analysis. We then applied this tool to identify 99 Acinetobacter clinical isolates from four public hospitals in Marseille, France. All isolates could easily be identified to species as they were separated into 13 species sequence types with a sequence variance of 0-2.6 % from their respective type strains. Of these 99 isolates, 10 were A. haemolyticus, 52 were A. baumannii, 27 were A. gen. sp. 3, 5 were A. schindleri, 1 was A. lwoffii, and 1 was A. gen. sp. 13TU. Three were provisionally identified as A. gen. sp. 9. This is the first work to identify all specimens of a set of clinical Acinetobacter isolates at species level using rpoB sequence analysis. Our data emphasize the recognition of A. schindleri as an emerging cause of Acinetobacter-related infection and confirm that A. gen. sp. 3 is the second most commonly isolated Acinetobacter species after A. baumannii in patients.

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Outbreak of a susceptible strain of Acinetobacter species 13 (sensu Tjernberg and Ursing) in an adult neurosurgical intensive care unit

Cited by 48 publications

References 28 publications

Nosocomial Acinetobacter Genomic Species 13 TU Endocarditis Following an Endoscopic Procedure

Nosocomial Acinetobacter Genomic Species 13 TU Endocarditis Following an Endoscopic Procedure

Identification of Acinetobacter Genomic Species 13TU by Sequence Analysis of the 16S-23S rRNA Gene Spacer Region

Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species

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