Out of Water of a New Model Legume: The Nod‐independent<i>Aeschynomene evenia</i>

Arrighi, Jean‐François; Cartieaux, Fabienne

doi:10.1002/9781119053095.ch45

Cited by 2 publications

(4 citation statements)

References 36 publications

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“…Genetic analysis in both Aeschynomene groups is now necessary to uncover the genetic determinism of stem nodulation. The diploid nature of the new model species A. evenia should facilitate such a genetic analysis (Arrighi & Cartieaux, ), but the African semiaquatic Aeschynomene will provide the opportunity to investigate whether paleopolyploidy might have provided genetic material leading or contributing to the evolution of stem nodulation, which is profuse in some species of this group.…”

Section: Discussionmentioning

confidence: 99%

The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene

et al. 2016

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SummaryThe legume genus Aeschynomene is notable in the ability of certain semiaquatic species to develop nitrogen-fixing stem nodules. These species are distributed in two clades. In the first clade, all the species are characterized by the use of a unique Nod-independent symbiotic process. In the second clade, the species use a Nod-dependent symbiotic process and some of them display a profuse stem nodulation as exemplified in the African Aeschynomene afraspera.To facilitate the molecular analysis of the symbiotic characteristics of such legumes, we took an integrated molecular and cytogenetic approach to track occurrences of polyploidy events and to analyze their impact on the evolution of the African species of Aeschynomene.Our results revealed two rounds of polyploidy: a paleopolyploid event predating the African group and two neopolyploid speciations, along with significant chromosomal variations. Hence, we found that A. afraspera (8x) has inherited the contrasted genomic properties and the stem-nodulation habit of its parental lineages (4x).This study reveals a comprehensive picture of African Aeschynomene diversification. It notably evidences a history that is distinct from the diploid Nod-independent clade, providing clues for the identification of the specific determinants of the Nod-dependent and Nodindependent symbiotic processes, and for comparative analysis of stem nodulation.

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Section: Discussionmentioning

confidence: 99%

The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene

et al. 2016

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“…Among the fluorescent calli, 23 (58%) derived from epicotyls and 4 (31%) from hypocotyls displayed the ability to develop shoots (see Table 2). Certain calli consistently produced a significant number of shoots (6)(7)(8)(9)(10)(11)(12), while others formed fewer shoots (1)(2)(3)(4)(5).…”

Section: Regeneration Of Transgenic Plantsmentioning

confidence: 99%

“…A . evenia was established as a valuable working model [ 7 ] and genomic resources have been recently generated including a high-quality genome sequence [ 8 ] and a gene expression atlas. Furthermore, a comprehensive examination of symbiotic genes revealed either the absence or the lack of expression of crucial genes involved in NodF perception and intracellular infection while the downstream components of the NodF signaling pathway were expressed [ 1 , 6 ] More recently, the screening of A .…”

Section: Introductionmentioning

confidence: 99%

“…Like other Aeschynomene spp, it is phylogenetically close to the cultivated peanut (Arachis hypogaea) but distant to M. truncatula and L. japonicus. A. evenia was established as a valuable working model [7] and genomic resources have been recently generated including a high-quality genome sequence [8] and a gene expression atlas. Furthermore, a comprehensive examination of symbiotic genes revealed either the absence or the lack of expression of crucial genes involved in NodF perception and intracellular infection while the downstream components of the NodF signaling pathway were expressed [1,6] More recently, the screening of A. evenia nodulation mutants confirmed the involvement of downstream components of the NodF signaling pathway [8].…”

Section: Introductionmentioning

confidence: 99%

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Setting up Agrobacterium tumefaciens-mediated transformation of the tropical legume Aeschynomene evenia, a powerful tool for studying gene function in Nod Factor-independent symbiosis

Tisseyre,

Cartieaux,

Chabrillange

et al. 2024

PLoS ONE

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Most legumes are able to develop a root nodule symbiosis in association with proteobacteria collectively called rhizobia. Among them, the tropical species Aeschynomene evenia has the remarkable property of being nodulated by photosynthetic Rhizobia without the intervention of Nod Factors (NodF). Thereby, A. evenia has emerged as a working model for investigating the NodF-independent symbiosis. Despite the availability of numerous resources and tools to study the molecular basis of this atypical symbiosis, the lack of a transformation system based on Agrobacterium tumefaciens significantly limits the range of functional approaches. In this report, we present the development of a stable genetic transformation procedure for A. evenia. We first assessed its regeneration capability and found that a combination of two growth regulators, NAA (= Naphthalene Acetic Acid) and BAP (= 6-BenzylAminoPurine) allows the induction of budding calli from epicotyls, hypocotyls and cotyledons with a high efficiency in media containing 0,5 μM NAA (up to 100% of calli with continuous stem proliferation). To optimize the generation of transgenic lines, we employed A. tumefaciens strain EHA105 harboring a binary vector carrying the hygromycin resistance gene and the mCherry fluorescent marker. Epicotyls and hypocotyls were used as the starting material for this process. We have found that one growth medium containing a combination of NAA (0,5 μM) and BAP (2,2 μM) was sufficient to induce callogenesis and A. tumefaciens strain EHA105 was sufficiently virulent to yield a high number of transformed calli. This simple and efficient method constitutes a valuable tool that will greatly facilitate the functional studies in NodF-independent symbiosis.

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Out of Water of a New Model Legume: The Nod‐independentAeschynomene evenia

Cited by 2 publications

References 36 publications

The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene

The evolutionary dynamics of ancient and recent polyploidy in the African semiaquatic species of the legume genus Aeschynomene

Setting up Agrobacterium tumefaciens-mediated transformation of the tropical legume Aeschynomene evenia, a powerful tool for studying gene function in Nod Factor-independent symbiosis

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