Ouabain-like immunoreactive substances exist in the hypothalamus and the adrenal medulla in rats

Takahashi, Hakuo; Ihara, Norihiko; Terano, Yoshitake; Yamada, Hisao; Nishimura, Masato; Nakanishi, Tadashi; Yamamoto, Kazumori; Kinoshita, Yoshihiro; Yoshimura, Manabu

doi:10.1016/s0928-4680(05)80006-9

Cited by 14 publications

(13 citation statements)

References 13 publications

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“…In spontaneously hypertensive rats (11) and Dahl salt-sensitive rats (12), the central nervous system seems to be the site where the OLC exerts its hypertensinogenic effects. In support of this view, Takahashi et al demonstrated by using mAb against ouabain (named T8B11) that ouabain-like immunoreactive substances predominantly exist in the paraventricular nucleus of the rat hypothalamus (13). Using the antibody, we noticed the existence of ouabain-like immunoreactivities in the rostral ventrolateral medulla (RVLM) of rats (14).…”

Section: Introductionsupporting

confidence: 52%

Role of ouabain-like compound in the rostral ventrolateral medulla in rats.

Teruya¹,

Yamazato²,

Muratani³

et al. 1997

J. Clin. Invest.

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To determine whether ouabain-like compound (OLC) exerts modulatory influences on the activity of vasomotor neurons in the rostral ventrolateral medulla (RVLM), we examined the effects of microinjecting ouabain, digoxin-specific antibody Fab fragments, and mAb against ouabain on the rat RVLM. Microinjection of ouabain into the unilateral RVLM of anesthetized normotensive rats elicited dose-dependent increases in mean arterial pressure (MAP) and renal sympathetic nerve activity (

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Section: Introductionsupporting

confidence: 52%

Role of ouabain-like compound in the rostral ventrolateral medulla in rats.

Teruya¹,

Yamazato²,

Muratani³

et al. 1997

J. Clin. Invest.

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“…In the present case, the OLI content in the adrenal medulla was slightly greater than in the cortex. OLI was found only in the adrenal medulla in our histochemical study with use of anti-ouabain monoclonal antibody (14). Therefore, the main source of circulating OLF could be the central nervous system, but based on our findings, the adrenal medulla/pheochromocytoma may also be the source.…”

Section: Discussionmentioning

confidence: 48%

“…Furthermore, OLI and DLI are distributed in the neurons of the paraventricular and supraoptic nuclei, and in the fibers containing the substance distributed to the circumventricular area of the third ventricle. This has been demonstrated by immunohistochemistry using mono-and poly-clonal antiouabain antibodies and polyclonal anti-digoxin antibody (1,(11)(12)(13). The OLI is also found in the adrenal medulla (14).…”

Section: Discussionmentioning

confidence: 86%

“…This has been demonstrated by immunohistochemistry using mono-and poly-clonal antiouabain antibodies and polyclonal anti-digoxin antibody (1,(11)(12)(13). The OLI is also found in the adrenal medulla (14). Since adrenal cortex contains an abundance of lipids, it may be hard to stain selectively in the cortex because tissue fixation is difficult by immunohistochemistry.…”

Section: Discussionmentioning

confidence: 98%

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Increases in Plasma Ouabainlike Immunoreactivity during Surgical Extirpation of Pheochromocytoma.

Komiyama¹,

Nishimura²,

Munakata³

et al. 1999

Hypertens Res

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The ouabainlike factor (OLF) is thought to be an important modulator of salt and water metabolism. Plasma OLF could be derived from the central nervous system and/or the adrenal gland. Since the adrenal medulla is of neural origin, the cytology of pheochromocytoma of adrenomedullary origin resembles that of neuronal cells. Ouabainlike immunoreactivity (OLI) is, in fact, present in the adrenal medulla as shown by immunohistochemistry.The plasma levels of catecholamines and OLI were significantly elevated during surgical extirpation of pheochromocytoma in this case. To clarify the origin of circulating OLI in a patient with pheochromocytoma, the relationship between plasma OLI and catecholamines during adrenalectomy was investigated. Plasma catecholamine levels exceeded the normal reference interval, and plasma OLI was positively correlated with the patient's plasma level of norepinephrine.The peak level during operation was about 10 times higher than the baseline level. Both levels reached a maximum when the tumor was mechanically pressed, and then gradually decreased thereafter.The level of OLI in the tumor was higher than that of the normal adrenal cortex. When OLI in the tumor was characterized by reversed-phase high-performance liquid chromatography, the retention time of OLI corresponded with that of authentic ouabain. These results suggest that the circulating OLI in this patients was derived mainly from the pheochromocytoma of adrenomedullary origin. (Hypertens Res 1999; 22: 135-139)

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“…This is partly due to structural similarities in the epitope of the antigen. In several studies using the immunohistochemical technique, with an anti-digoxin monoclonal antibody, the digitalis-like immunoreactive substances were localized in the hypothalamus or the adrenal medulla [18][19][20][21] . We previously described a histochemical technique to localize the immunoreactive products of anti-digoxin monoclonal antibodies in patients who were chronically treated with digoxin [17] .…”

Section: Identification Of Digitalis-like Immunoreactive Substancesmentioning

confidence: 99%

Increased digitalis-like immunoreactive substances in patients with hypertrophic cardiomyopathy

Hayashi

Ijiri²,

Toko³

et al. 2000

European Heart Journal

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Aims Although increased digitalis-like immunoreactive substances have been found in cases of hypertension and heart failure, no information is available about digitalis-like immunoreactive substances in patients with hypertrophic cardiomyopathy. We investigated digitalis-like immunoreactive substances in the plasma and biopsied specimens of patients with hypertrophic cardiomyopathy. Methods and ResultsIn 40 patients with hypertrophic cardiomyopathy (27 with the non-obstructive type and 13 with the obstructive type), the plasma concentration of digitalis-like immunoreactive substances was studied by fluorescence polarization immunoassay. Right ventricular endomyocardial biopsy specimens were analysed immunohistochemically, using a monoclonal antibody against digoxin. An increase in digitalis-like immunoreactive substances of more than 0·2 ng . ml 1 in plasma was found in six of 27 patients with non-obstructive hypertrophic cardiomyopathy (22·2%) and five of 13 with obstructive hypertrophic cardiomyopathy (38·4%). Under light microscopy, positive staining against the antibody was observed heterogeneously on some cardiocytes. In non-obstructive hypertrophic cardiomyopathy, digitalis-like immunoreactive substances in the plasma correlated with the left atrial dimension and inversely with the cardiac index. In obstructive hypertrophic cardiomyopathy, plasma and myocardial digitalis-like immunoreactive substances were positively correlated; they also correlated with left ventricular enddiastolic pressures. Under electron microscopy, digitalislike immunoreactive substances were detected at the sarcolemma in the free wall, T-tubules, intercalated discs and Z-bands of cardiocytes.Conclusions Increased digitalis-like immunoreactive substances in plasma and cardiocytes, which may have been caused by pressure and/or volume overload, were found in patients with hypertrophic cardiomyopathy. Digitalis-like immunoreactive substances may act on the sarcolemma of cardiocytes and be transported into the cytoplasm.

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Ouabain-like immunoreactive substances exist in the hypothalamus and the adrenal medulla in rats

Cited by 14 publications

References 13 publications

Role of ouabain-like compound in the rostral ventrolateral medulla in rats.

Role of ouabain-like compound in the rostral ventrolateral medulla in rats.

Increases in Plasma Ouabainlike Immunoreactivity during Surgical Extirpation of Pheochromocytoma.

Increased digitalis-like immunoreactive substances in patients with hypertrophic cardiomyopathy

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