Ouabain-insensitive Na+-ATPase of proximal tubules is an effector for urodilatin and atrial natriuretic peptide

Caruso-Neves, Celso; Vives, D.; Dantas, Camila; Albino, Cátia; Fonseca, Leonardo Marques da; Lara, Lucienne S.; Iso, Manami; Lopes, Anibal Gil

doi:10.1016/j.bbamem.2003.11.002

Cited by 26 publications

(8 citation statements)

References 25 publications

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“…A significant reduction in the urine ANP concentration was observed in the urine of rats treated with SAP Aa D. Therefore, we cannot exclude the possibility that the increase in renal ATPase activity induced by SAP Aa D was a result of the reduction in the renal urodilatin level. This explanation is, at least in part, consistent with the observation that renal urodilatin inhibits Na + -ATPase activity but not (Na + ,K + )-ATPase activity in proximal tubules [37]. However, in a previous study [38], it was shown that exogenous urodilatin dosed at 10 nM increased the (Na + ,K + )-ATPase activity in the renal outer cortex of Sprague–Dawley rats.…”

Section: Discussionsupporting

confidence: 86%

The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

Diniz

Portella

Cardoso

et al. 2012

BMC Complement Altern Med

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BackgroundIn a previous study, we showed that a saponin mixture isolated from the roots of Ampelozizyphus amazonicus Ducke (SAPAaD) reduces urine excretion in rats that were given an oral loading of 0.9 % NaCl (4 ml/100 g body weight). In the present study, we investigated whether atrial natriuretic peptides (ANP) and renal ATPases play a role in the SAPAaD- induced antidiuresis in rats.MethodsTo evaluate the effect of SAPAaD on furosemide-induced diuresis, Wistar rats (250-300 g) were given an oral loading of physiological solution (0.9 % NaCl, 4 ml/100 g body weight) to impose a uniform water and salt state. The solution containing furosemide (Furo, 13 mg/kg) was given 30 min after rats were orally treated with 50 mg/kg SAPAaD (SAPAaD + Furo) or 0.5 ml of 0.9 % NaCl (NaCl + Furo). In the SAPAaD + NaCl group, rats were pretreated with SAPAaD and 30 min later they received the oral loading of physiological solution. Animals were individually housed in metabolic cages, and urine volume was measured every 30 min throughout the experiment (3 h). To investigate the role of ANP and renal Na+ pumps on antidiuretic effects promoted by SAPAaD, rats were given the physiological solution (as above) containing SAPAaD (50 mg/kg). After 90 min, samples of urine and blood from the last 30 min were collected. Kidneys and atria were also removed after previous anesthesia. ANP was measured by radioimmunoassay (RIA) and renal cortical activities of Na+- and (Na+,K+)-ATPases were calculated from the difference between the [32P] Pi released in the absence and presence of 1 mM furosemide/2 mM ouabain and in the absence and presence of 1 mM ouabain, respectively.ResultsIt was observed that SAPAaD inhibited furosemide-induced diuresis (at 90 min: from 10.0 ± 1.0 mL, NaCl + Furo group, n = 5, to 5.9 ± 1.0 mL, SAPAaD + Furo group n = 5, p < 0.05), increased both Na+-ATPase (from 25.0 ± 5.9 nmol Pi.mg-1.min-1, control, to 52.7 ± 8.9 nmol Pi.mg-1.min-1, p < 0.05) and (Na+,K+)-ATPase (from 47.8 ± 13.3 nmol Pi.mg-1.min-1, control, to 79.8 ± 6.9 nmol Pi .mg-1.min-1, p < 0.05) activities in the renal cortex. SAPAaD also lowered urine ANP (from 792 ± 132 pg/mL, control, to 299 ± 88 pg/mL, p < 0.01) and had no effect on plasma or atrial ANP.ConclusionWe concluded that the SAPAaD antidiuretic effect may be due to an increase in the renal activities of Na+- and (Na+,K+)-ATPases and/or a decrease in the renal ANP.

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Section: Discussionsupporting

confidence: 86%

The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

Diniz

Portella

Cardoso

et al. 2012

BMC Complement Altern Med

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“…Atrial natriuretic peptide (ANP) and urodilatin specifically inhibit the Na + -ATPase activity by activating the PKG pathway through the natriuretic peptide receptor (NPR-A) located in the luminal and basolateral membranes of proximal tubular cells [28, 176]. …”

Section: Modulation Of the Na+-atpase Activitymentioning

confidence: 99%

The second sodium pump: from the function to the gene

Rocafull

Thomas

Castillo

2012

Pflugers Arch - Eur J Physiol

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Transepithelial Na+ transport is mediated by passive Na+ entry across the luminal membrane and exit through the basolateral membrane by two active mechanisms: the Na+/K+ pump and the second sodium pump. These processes are associated with the ouabain-sensitive Na+/K+-ATPase and the ouabain-insensitive, furosemide-inhibitable Na+-ATPase, respectively. Over the last 40 years, the second sodium pump has not been successfully associated with any particular membrane protein. Recently, however, purification and cloning of intestinal α-subunit of the Na+-ATPase from guinea pig allowed us to define it as a unique biochemical and molecular entity. The Na+- and Na+/K+-ATPase genes are at the same locus, atp1a1, but have independent promoters and some different exons. Herein, we spotlight the functional characteristics of the second sodium pump, and the associated Na+-ATPase, in the context of its role in transepithelial transport and its response to a variety of physiological and pathophysiological conditions. Identification of the Na+-ATPase gene (atna) allowed us, using a bioinformatics approach, to explore the tertiary structure of the protein in relation to other P-type ATPases and to predict regulatory sites in the promoter region. Potential regulatory sites linked to inflammation and cellular stress were identified in the atna gene. In addition, a human atna ortholog was recognized. Finally, experimental data obtained using spontaneously hypertensive rats suggest that the Na+-ATPase could play a role in the pathogenesis of essential hypertension. Thus, the participation of the second sodium pump in transepithelial Na+ transport and cellular Na+ homeostasis leads us to reconsider its role in health and disease.

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“…This transporter is an important homeostatic target of brain and circulating signals for fi ne regulation of Na + reabsorption. For example, it has been demonstrated that ANP inhibits the proximal Na + -ATPase by the NPR-TO/guanilate cyclase/ cGMP pathway (Harris et al 1996, Caruso-Neves et al 2004. Similarly, natriuretic effect of OT is dependent on guanilate cyclase/cGMP pathway but through NO synthesis (Soares et al 1999).…”

Section: Renin-angiotensin-aldosterone Anp and Oxytocin Systems And mentioning

confidence: 99%

Role of the serotoninergic system in the sodium appetite control

Reis

2007

An. Acad. Bras. Ciênc.

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The present article reviews the role of the serotoninergic system in the regulation of the sodium appetite. Data from the peripheral and icv administration of serotoninergic (5-HTergic) agents showed the participation of 5-HT2/3 receptors in the modulation of sodium appetite. These observations were extended with the studies carried out after brain serotonin depletion, lesions of DRN and during blockade of 5-HT2A/2C receptors in lateral parabrachial nucleus (LPBN). Brain serotonin depletion and lesions of DRN increased the sodium appetite response, in basal conditions, after sodium depletion and hypovolemia or after beta-adrenergic stimulation as well. These observations raised the hypothesis that the suppression of ascending pathways from the DRN, possibly, 5-HTergic fi bers, modifi es the angiotensinergic or sodium sensing mechanisms of the subfornical organ involved in the control of the sodium appetite. 5-HTergic blockade in LPBN induced to similar results, particularly those regarded to the natriorexigenic response evoked by volume depletion or increase of the hypertonic saline ingestion induced by brain angiotensinergic stimulation. In conclusion, many evidences lead to acceptation of an integrated participation resulting of an interaction, between DRN and LPBN, for the sodium appetite control.

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Ouabain-insensitive Na+-ATPase of proximal tubules is an effector for urodilatin and atrial natriuretic peptide

Cited by 26 publications

References 25 publications

The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

The effect of saponins from Ampelozizyphus amazonicus Ducke on the renal Na+ pumps’ activities and urinary excretion of natriuretic peptides

The second sodium pump: from the function to the gene

Role of the serotoninergic system in the sodium appetite control

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