Ouabain-insensitive effects of metabolism on ion and water content of red blood cells

Jc, Parker

doi:10.1152/ajplegacy.1971.221.1.338

Cited by 34 publications

(24 citation statements)

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“…The density change may also be considered in terms of continued hemoglobin synthesis as recently shown for red cells of anemic dogs (Kirk et al, 1978). In addition, it is known that elevated levels of impermeable organophosphate anions cause a chloride and water shift out of the cell, which results in celt volume reduction (Parker, 1971). Hence, elevated levels of adenosine triphosphate, glutamate, and glutathione may also contribute to volume and density changes.…”

Section: Discussionmentioning

confidence: 98%

LK sheep reticulocytosis: effect of anti-L on K influx and in vitro maturation.

Kim¹,

Theg²,

Lauf³

1980

The Journal of General Physiology

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After massive hemorrhage, adult sheep with genotypically low potassium (LK) red cells temporarily produce high potassium (HK) cells with ouabain-sensitive K + pump fluxes equivalent to mature HK red cells. In light of recent reports of different red cell volume populations accompanying the HK-LK transition also occurring in newborn LK sheep and the unresolved controversy over the effect of anti-L on K + transport in these immature red cells, we have reexamined the K + transport changes and the effect of anti-L in the newly formed HK cells at various times after anemic stress and under in vitro conditons. We found that ~7 d after bleeding, maximum reticulocytosis occurred in the peripheral blood. After separation by density centrifugation, the top 10% cell fraction contained 100% reticulocytes, with a mean cell volume 2.5 times larger than that of mature erythrocytes. These immature red cells were of HK type, and their K + pump and leak fluxes were 30 and 10 times higher, respectively, than those found in mature LK cells. The new cells may possess HK-and LK-type pumps because K + pump influx was significantly stimulated by anti-L. When separated by density centrifugation on days 9, 17, and 23 after bleeding, some of the cells apparently maintained their large size while gaining higher density. Large cells from day 9, kept in vitro for 22 h, showed anti-Lsensitive K + pump and leak fluxes that declined within hours, paralleling the behavior of these cells in vivo, whereas cellular K + levels changed much less. It is concluded that the newly formed red cells may belong to a stress-induced macrocytic cell population that does not acquire all of the characteristics of adult LK cells.

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Section: Discussionmentioning

confidence: 98%

LK sheep reticulocytosis: effect of anti-L on K influx and in vitro maturation.

Kim¹,

Theg²,

Lauf³

1980

The Journal of General Physiology

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“…All RBC incubations were at 37°C in suspensions having a cell/medium ratio of ~0 to ~0 At each sampling time some of the suspension was removed, and in preparation for sodium, potassium, and water measurements the ccUs were washed four times by repeated centrifugation at room temperature in solutions which contained glycylglycine 5 mM (pH 7.4) plus sufficient NaC1 to bring the freezing point of the wash solution to that of the medium in which the cells had been incubated. Methods for cell sodium, potassium, dry weight (DW), and water have been previously reported from this laboratory (13)(14)(15)(16). Cells were prepared for calcium determinations by washing four times at room temperature with a solution containing (mM): NaCI 140, glycylglycine 5, pH 7.35.…”

mentioning

confidence: 99%

Role of calcium in volume regulation by dog red blood cells.

Parker¹,

Gitelman²,

Glosson³

et al. 1975

The Journal of General Physiology

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Dog red blood cells (RBC) are shown to regulate their volume in anisosmotic media. Extrusion of water from osmotically swollen cells requires external calcium and is associated with net outward sodium movement. Ac= cumulation of water by osmotically shrunken cells is not calcium dependent and is associated with net sodium uptake. Net movements of calcium are influenced by several variables including cell volume, pH, medium sodium concentration, and cellular sodium concentration. Osmotic swelling of cells increases calcium permeability, and this effect is diminished at acid pH. Net calcium flux in either direction between cells and medium is facilitated when the sodium concentration is low in the compartment from which calcium moves and/or high in the compartment to which calcium moves. The hypothesis is advanced that energy for active sodium extrusion in dog RBC comes from passive, inward flow of calcium through a countertransport mechanism.

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“…A cell to medium ratio of approximately 1:20 was maintained in all experiments. For cell Ca determinations the cells were packed by the centrifuge method previously described by Parker (1971). Since erythrocytes do not accumulate Ca in the absence of ionophore, we used the Ca values of nonionophore-treated samples to correct for the trapping of extracellular fluid.…”

Section: Methodsmentioning

confidence: 99%

“…Cellular concentrations are expressed in terms of hemoglobin so the units of measure are independent of any alterations in cell volume. Cell water determinations were done by drying to constant weight (Parker 1971). Red celI ghosts were prepared by hemolyzing cells in a cold 0.1 mM EDTA solution, followed by repeated washes in 15 mM sodium chloride solutions containing 1.7 mM tris buffer, pH 7.4.…”

Section: Methodsmentioning

confidence: 99%

Calcium dependent ATP losses in intact red blood cells without cellular accumulations of calcium

Plishker

Gitelman

1977

J. Membrain Biol.

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Intact human red blood cells incubated with ionophore A23187 and calcium develop a depletion of ATP that is dependent upon the concentrations of both A23187 and Ca. Incubations of fresh cells with 0.5 micrometer A23187 and concentrations of Ca at or below 70 micrometer produce a depletion of ATP without a net cellular uptake of Ca. In contrast, ATP-depleted cells display an ionophore-dependent cellular uptake of Ca, under identical conditions. A hypothesis is proposed that relates these ionophore-produced ATP depletions to active Ca extrusion by the Ca ATPase.

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Ouabain-insensitive effects of metabolism on ion and water content of red blood cells

Cited by 34 publications

References 0 publications

LK sheep reticulocytosis: effect of anti-L on K influx and in vitro maturation.

LK sheep reticulocytosis: effect of anti-L on K influx and in vitro maturation.

Role of calcium in volume regulation by dog red blood cells.

Calcium dependent ATP losses in intact red blood cells without cellular accumulations of calcium

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