Otx2, Gbx2, and Fgf8 expression patterns in the chick developing inner ear and their possible roles in otic specification and early innervation

Sánchez-Calderón, Hortensia; Martı́n-Partido, Gervasio; Hidalgo‐Sánchez, Matías

doi:10.1016/j.modgep.2004.04.008

Cited by 35 publications

(45 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Null mutations of Ngn1 and NeuroD in mice show that these genes are necessary for neuronal determination and differentiation (Ma et al, 2000;Kim et al, 2001;Matei et al, 2005). Cell communication signals are also expressed in this domain, including several FGFs, which have been shown to be required for neuronal production (Alsina et al, 2004;Sanchez-Calderon et al, 2004. Other genes are expressed in the complementary posterior and dorsal domains of the otic cup and vesicle (Alsina et al, 2004;Abelló et al, 2006).…”

Section: Sox2 and The State Of Commitment Of Sensory Progenitorsmentioning

confidence: 99%

Differential expression of Sox2 and Sox3 in neuronal and sensory progenitors of the developing inner ear of the chick

Neves

Kamaid

Alsina

et al. 2007

J of Comparative Neurology

129

View full text Add to dashboard Cite

The generation of the mechanosensory elements of the inner ear during development proceeds in a precise temporal and spatial pattern. First, neurosensory precursors form sensory neurons. Then, prosensory patches emerge and give rise to hair and supporting cells. Hair cells are innervated by cochleovestibular neurons that convey sound and balance information to the brain. SOX2 is an HMG transcription factor characteristic of the stem-cell genetic network responsible for progenitor self-renewal and commitment, and its loss of function generates defects in ear sensory epithelia. The present study shows that SOX2 protein is expressed in a spatially and temporally restricted manner throughout development of the chick inner ear. SOX2 is first expressed in the neurogenic region that gives rise to sensory neurons. SOX2 is then restricted to the prosensory patches in E4 and E5 embryos, as revealed by double and parallel labelling with SOX2 and Tuj1, MyoVIIa, or Islet1. Proliferating cell nuclear antigen labelling showed that SOX2 is expressed in proliferating cells during those stages. By E5, SOX2 is also expressed in the Schwann cells of the cochleovestibular ganglion, but not in the otic neurons. At E8 and E17, beyond stages of sensory cell specification, SOX2 is transiently expressed in hair cells, but its level remains high in supporting cells. SOX3 is concomitantly expressed with SOX2 in the neurogenic domain of the otic cup, but not in prosensory patches. Our data are consistent with a role for SOX2 in specifying a population of otic progenitors committed to a neural fate, giving rise to neurons and hair cells.

show abstract

Section: Sox2 and The State Of Commitment Of Sensory Progenitorsmentioning

confidence: 99%

Differential expression of Sox2 and Sox3 in neuronal and sensory progenitors of the developing inner ear of the chick

Neves

Kamaid

Alsina

et al. 2007

J of Comparative Neurology

129

View full text Add to dashboard Cite

show abstract

“…Only the presumptive domain of the macula neglecta and the lateral crista start from being Fgf10 negative to become Fgf10 positive after their specification as sensory elements (Sánchez-Guardado et al 2013). Also, Fgf8 could participate in the arrangement of the macula sacculi and macula lagena, as well as in the specification of the medial part of the macula utriculi (Sánchez-Calderón et al 2002, 2004). Besides, Fgf19 expression could regulate the development of both the macula utriculi and the macula lagena (Sánchez-Calderón et al 2007a; Sánchez-Guardado et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…It has been used as a marker for differentiating neurons in the developing chick nervous system (Yamada et al 1991; Storey et al 1992; Hill et al 1995; Perez et al 1999). In the developing inner ear, the 3A10 antibody is a useful tool to label axons of the acoustic-vestibular ganglion (AVG) neurons and to aid in the identification of presumptive sensory epithelia (Adam et al 1998; Sánchez-Calderón et al 2004, 2005, 2007b; Battisti and Fekete 2008; Sánchez-Guardado et al 2009, 2011, 2013; see also Sienknecht and Fekete 2008, 2009). …”

Section: Methodsmentioning

confidence: 99%

“…At the otic vesicle stage, the Fgf8 expression defines a continuous rostrocaudal band located within the Fgf10 -expressing domain, contiguous to the Gbx2 domain, and overlapping the Otx2 domain at caudal levels (Hidalgo-Sánchez et al 2000; Sánchez-Guardado et al 2013). This Fgf8 -positive patch observed in the otocyst wall could participate in the specification of the macula sacculi and macula lagena, as well as in the medial part of the macula utriculi (Sánchez-Calderón et al 2002, 2004). Also, Fgf19 expression is present in the developing macula utriculi and macula lagena, as well as in the borders of various developing sensory elements (Sánchez-Calderón et al 2007a; Sánchez-Guardado et al 2013).…”

Section: Introductionmentioning

confidence: 94%

“…Final cell fate specifications, differential growth, and morphogenesis in the developing membranous labyrinth could occur through asymmetric transcription factor expression patterns, which provide positional identities according to a compartmental model (Fekete 1996; Brigande et al 2000; Fekete and Wu 2002). A complex network of morphogenes, such as FGF, RA, WNT, and BMP, determine directly the regionalization of the developing membranous labyrinth (for the chick inner ear, see: Oh et al 1996; Wu and Oh 1996; Sánchez-Calderón et al 2004, 2007a; Stevens et al 2003; Sienknecht and Fekete 2008, 2009; Sánchez-Guardado et al 2009; 2013). Despite the interest existing about the molecular and cellular mechanisms involved in inner ear patterning and fate specification of the growing membranous labyrinth, a high number of questions remain to be resolved.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fgf3 and Fgf16 expression patterns define spatial and temporal domains in the developing chick inner ear

et al. 2016

View full text Add to dashboard Cite

The inner ear is a complex sensorial structure with auditory and vestibular functions. The developing otic epithelium gives rise to neurosensory and non-sensory elements of the adult membranous labyrinth. Extrinsic and intrinsic signals manage the patterning and cell specification of the developing otic epithelium by the establishment of lineage-restricted compartments defined in turn by differential expressions of regulatory genes. FGF3 and FGF16 are excellent candidates to govern these developmental events. In the chick, we showed that Fgf3 expression was present in the borders of all developing cristae. Strong Fgf16 expressions were detected in a portion of the developing vertical and horizontal pouches, whereas the cristae showed weaker or undetected Fgf16 expressions at different developmental stages. Concerning the rest of the vestibular sensory elements, both the utricular and saccular maculae were Fgf3 positive. Interestingly, a strong Fgf16 expression delimited these Fgf16-negative sensory patches. The Fgf3-negative macula neglecta and Fgf3-positive macula lagena were included within weakly Fgf16-expressing areas. Therefore, different FGF-mediated mechanisms might regulate the specification of the anterior (utricular and saccular) and posterior (neglecta and lagena) maculae. In the developing cochlear duct, the dynamic Fgf3 and Fgf16 expressions suggest their co-operation in the early specification and later cell differentiation in the hearing system. The requirement of Fgf3 and Fgf16 genes in endolymphatic apparatus development and neurogenesis are discussed. Based on these observations, FGF3 and FGF16 appear to be key signaling pathways to control the inner ear plan by means of defining epithelial identities within the developing otic epithelium.

show abstract

AMN107, a novel aminopyrimidine inhibitor of p190 Bcr‐Abl activation and of in vitro proliferation of Philadelphia‐positive acute lymphoblastic leukemia cells

Verstovšek

Golemović

Kantarjian

et al. 2005

Cancer

View full text Add to dashboard Cite

BACKGROUNDPrevious studies have shown that patients with Bcr‐Abl–positive acute lymphoblastic leukemia (ALL) either have primary disease that is refractory to imatinib mesylate or develop disease recurrence after an initial response.METHODSThe authors investigated the effects of a newly designed Bcr‐Abl inhibitor, AMN107, by comparing its in vitro inhibitory potency on p190 Bcr‐Abl ALL cell lines with that of imatinib.RESULTSIn two Philadelphia (Ph)‐positive ALL cell lines, AMN107 was found to be 30–40 times more potent than imatinib in inhibiting cellular proliferation. AMN107 was also more effective than imatinib in inhibiting phosphorylation of p190 Bcr‐Abl tyrosine kinase in cell lines and primary ALL cells. The inhibition of cellular proliferation was associated with the induction of apoptosis in only one of the cell lines. No activity was observed in cell lines lacking the BCR‐ABL genotype.CONCLUSIONSThe results of the current study suggest the superior potency of AMN107 compared with imatinib in Ph‐positive ALL and support clinical trials of AMN107 in patients with Ph‐positive ALL. Cancer 2005. © 2005 American Cancer Society.

show abstract

Otx2, Gbx2, and Fgf8 expression patterns in the chick developing inner ear and their possible roles in otic specification and early innervation

Cited by 35 publications

References 30 publications

Differential expression of Sox2 and Sox3 in neuronal and sensory progenitors of the developing inner ear of the chick

Differential expression of Sox2 and Sox3 in neuronal and sensory progenitors of the developing inner ear of the chick

Fgf3 and Fgf16 expression patterns define spatial and temporal domains in the developing chick inner ear

AMN107, a novel aminopyrimidine inhibitor of p190 Bcr‐Abl activation and of in vitro proliferation of Philadelphia‐positive acute lymphoblastic leukemia cells

Contact Info

Product

Resources

About