OTU-022 Gwas of microscopic colitis in the UK biobank confirms association within the HLA region

Abstract

symptom response (adequate relief), gut microbiome (16S rRNA sequencing), short-chain fatty acids (SCFA, gas liquid chromatography), volatile organic compounds (VOC) (gas chromatography/mass spectrometry) and urine metabolomics (NMR spectroscopy) at baseline (BL) and 4 weeks. Data were analysed by Chi 2 for dichotomous outcomes and Kruskal-Wall-isMann-Whitney U tests for continuous variables. Predictive analysis was tested using receiver operator curves (ROC) and orthoganol partial least squared discriminant analysis (OPLS-DA). Results Response rate (adequate relief) at 4 weeks was greater in the LFD+GOS (67%) and LFD (50%) than the Control group (30%) (p=0.046). The phylum Actinobacteria was lower (p<0.001), specifically Bifidobacterium (p=0.002) and Collinsella (p=0.004), in the LFD and LFD+B GOS groups compared to Control at 4 weeks. Prediction of response to the LFD was seen in the LFD group only, with significant separation of BL faecal VOC profiles (AUC=0.854, p=0.045), faecal propionate (AUC=0.848, p=0.009), and urine metabolite profiles (Q 2 =0.296 vs randomised-0.175). Roseburia, Blautia, Lachnospira and Faecalibacterium, were positively but not significantly associated with VOC classes that predicted response when all samples were correlated. Microbiome and VOC showed a significant negative correlation between propionic acid derivatives and Phascolarctobacterium (r=-0.85 and-0.81, p<0.05) at BL in the LFD group only although the difference in Phascolarctobacterium between responders (1.5%) and nonresponders (9.3%) at BL did not reach significance (p=0.055). Conclusions The LFD +B GOS improves symptoms in IBS compared to Control but the LFD significantly reduces Actinobacteria and addition of 1.4 g/d of prebiotic B-GOS does not overcome this effect. Faecal VOCs and SCFAs and urine metabolomes may predict clinical response to the LFD, and specific bacterial groups correlate with predictive metabolites. Larger studies are required to validate these algorithms to develop personalised nutrition in IBS.

Short-term duration of diabetic retinopathy as a predictor for development of diabetic kidney disease

“…The UK Biobank further confirmed its association with the human leukocyte antigen (HLA) region. [ 37 ] The rs 10737680 is also reported to be associated with interleukin-6 receptor. [ 38 ] These results may provide evidence for the causal role of DR in the development of DKD, since inflammatory factors cause lesions in renal function in patients with DR, while the effects of confounding factors are minimized.…”