Ottowia shaoguanensis sp. nov., Isolated From Coking Wastewater

Geng, Shuang; Pan, Xin-Chi; Mei, Renwei; Wang, Yanan; Sun, Junsong; Liu, Xueying; Tang, Yue‐Qin; Wu, Xiao‐Lei

doi:10.1007/s00284-013-0481-8

Cited by 31 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the anaerobic sludge, the facultative aerobic Gram-negative bacterium Ottowia was the most abundant genus, which differed from previous studies Zhu et al 2016). Though Ottowia has been isolated from activated sludge treating CWW wastewater (Felfoldi et al 2011;Cao et al 2014;Geng et al 2014), its presence at such a high abundance in anaerobic sludge was not reported previously. The presence of Ottowia together with Advenella Ghosh et al 2011), Corynebacterium (Dalal et al 2012;Guo et al 2015), and Sphingobium (Kertesz and Kawasaki 2010) might be associated with the removal of phenols, NHCs as well as PAHs under the anaerobic conditions (Table S8).…”

Section: Discussioncontrasting

confidence: 73%

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The combined anaerobic-aerobic biosystem is assumed to consume less energy for the treatment of high strength industrial wastewater. In this study, pollutant removal performance and microbial diversity were assessed in a longterm (over 300 days) bench-scale sequential anaerobicaerobic bioreactor treating coking wastewater. Anaerobic treatment removed one third of the chemical oxygen demand (COD) and more than half of the phenols with hydraulic retention time (HRT) of 42 h, while the combined system with total HRT of 114 h removed 81.8, 85.6, 99.9, 98.2, and 85.4 % of COD, total organic carbon (TOC), total phenols, thiocyanate, and cyanide, respectively. Two-dimensional gas chromatography with time-of-flight mass spectrometry showed complete removal of phenol derivatives and nitrogenous heterocyclic compounds (NHCs) via the combined system, with the anaerobic process alone contributing 58.4 and 58.6 % removal on average, respectively. Microbial activity in the bioreactors was examined by 454 pyrosequencing of the bacterial, archaeal, and fungal communities. Proteobacteria (61.2-93.4 %), particularly Betaproteobacteria (34.4-70.1 %), was the dominant bacterial group. Ottowia (14.1-46.7 %), Soehngenia (3.0-8.2 %), and Corynebacterium (0.9-12.0 %), which are comprised of phenol-degrading and hydrolytic bacteria, were the most abundant genera in the anaerobic sludge, whereas Thiobacillus (6.6-43.6 %), Diaphorobacter (5.1-13.0 %), and Comamonas (0.2-11.1 %) were the major degraders of phenol, thiocyanate, and NHCs in the aerobic sludge. Despite the low density of fungi, phenol degrading oleaginous yeast Trichosporon was abundant in the aerobic sludge. This study demonstrated the feasibility and optimization of less energy intensive treatment and the potential association between abundant bacterial groups and biodegradation of key pollutants in coking wastewater.

show abstract

Section: Discussioncontrasting

confidence: 73%

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Joshi

Zhang

Tian

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…It was not detected in any background technical controls. Ottowia is a genus of bacteria that has been isolated from industrial and municipal wastewater (96)(97)(98)(99), sikhye (100), tofu residue (101), and fish intestines (102); it has not been identified in investigations of the human uterus. Nevertheless, Ottowia is a member of the family Comamonadaceae, and Chen et al ( 23) and Winters et al (30) reported that Comamonadaceae were among the most relatively abundant bacterial taxa in the human endometrium.…”

Section: Discussionmentioning

confidence: 99%

Lack of Evidence for Microbiota in the Placental and Fetal Tissues of Rhesus Macaques

Theis

Romero

Winters

et al. 2020

mSphere

View full text Add to dashboard Cite

The prevailing paradigm in obstetrics has been the sterile womb hypothesis. However, some are asserting that the placenta, intra-amniotic environment, and fetus harbor microbial communities. The objective of this study was to determine whether the fetal and placental tissues of rhesus macaques harbor bacterial communities. Fetal, placental, and uterine wall samples were obtained from cesarean deliveries without labor (∼130/166 days gestation). The presence of bacteria in the fetal intestine and placenta was investigated through culture. The bacterial burden and profiles of the placenta, umbilical cord, and fetal brain, heart, liver, and colon were determined through quantitative real-time PCR and DNA sequencing. These data were compared with those of the uterine wall as well as to negative and positive technical controls. Bacterial cultures of fetal and placental tissues yielded only a single colony of Cutibacterium acnes. This bacterium was detected at a low relative abundance (0.02%) in the 16S rRNA gene profile of the villous tree sample from which it was cultured, yet it was also identified in 12/29 background technical controls. The bacterial burden and profiles of fetal and placental tissues did not exceed or differ from those of background technical controls. By contrast, the bacterial burden and profiles of positive controls exceeded and differed from those of background controls. Among the macaque samples, distinct microbial signals were limited to the uterine wall. Therefore, using multiple modes of microbiologic inquiry, there was not consistent evidence of bacterial communities in the fetal and placental tissues of rhesus macaques. IMPORTANCE Microbial invasion of the amniotic cavity (i.e., intra-amniotic infection) has been causally linked to pregnancy complications, especially preterm birth. Therefore, if the placenta and the fetus are typically populated by low-biomass microbial communities, current understanding of the role of microbes in reproduction and pregnancy outcomes will need to be fundamentally reconsidered. Could these communities be of benefit by competitively excluding potential pathogens or priming the fetal immune system for the microbial bombardment it will experience upon delivery? If so, what properties (e.g., microbial load and community membership) of these microbial communities preclude versus promote intra-amniotic infection? Given the ramifications of the in utero colonization hypothesis, critical evaluation is required. In this study, using multiple modes of microbiologic inquiry (i.e., culture, quantitative real-time PCR [qPCR], and DNA sequencing) and controlling for potential background DNA contamination, we did not find consistent evidence for microbial communities in the placental and fetal tissues of rhesus macaques.

show abstract

“…They were isolated from diverse environments including soil, freshwater, groundwater, marine environments, swamps, sewage, activated sludge, plant rhizospheres, nitrifying inocula, chickens, saline pond, and lake sediment [7,10,36,37]. During the investigation into the diversity of phenol-degrading bacteria in a coking wastewater [8], a strain designed as J5-3 T was isolated and identified. Based on the physiological, biochemical, and phylogenetic analyses, strain J5-3 T represents a novel genus belonging to the family Hyphomicrobiaceae.…”

Section: Introductionmentioning

confidence: 99%

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

et al. 2014

Self Cite

View full text Add to dashboard Cite

A Gram staining negative, rod-shaped, aerobic bacterial strain J5-3(T) with a single polar flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was motile and capable of optimal growth at pH 6-8, 30 °C, and 0-2 % (w/v) NaCl. Its predominant fatty acids were 11-methyl C18:1 ω7c (29.2 %), C16:0 (20.6 %), C19:0 cyclo ω8c (18.2 %), C18:0 (11.0 %), and C18:1 ω7c/C18:1 ω6c (10.9 %) when grown on trypticase soy agar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids (GL1, GL2), and two unknown phospholipid (PL1, PL2). The predominant ubiquinone was Q-10, and the genome DNA G+C content was 61.7 mol %. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain J5-3(T) belonged to the family Hyphomicrobiaceae in Alphaproteobacteria. It shared the 16S rRNA gene sequence similarities of 93.8-96.1 % with the genus Devosia, 94.5-94.8 % with the genus Pelagibacterium, and <92.0 % with all the other type strains in family Hyphomicrobiaceae. It can be distinguished from the closest phylogenetic neighbors based on several phenotypic and genotypic features, including α-galactosidase activity, tetracycline susceptibility, major fatty acid composition, polar lipid profile, DNA gyrase B subunit (gyrB) gene sequence, and random-amplified polymorphic DNA profile. Therefore, we consider strain J5-3(T) to represent a novel species of a novel genus within the family Hyphomicrobiaceae, for which the name Paradevosia shaoguanensis gen. nov., sp. nov. is proposed. The type strain of Paradevosia shaoguanensis is J5-3(T) (=CGMCC 1.12430(T) =LMG 27409(T)).

show abstract

Ottowia shaoguanensis sp. nov., Isolated From Coking Wastewater

Cited by 31 publications

References 19 publications

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Performance and microbial community composition in a long-term sequential anaerobic-aerobic bioreactor operation treating coking wastewater

Lack of Evidence for Microbiota in the Placental and Fetal Tissues of Rhesus Macaques

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

Contact Info

Product

Resources

About