Ottowia konkukae sp. nov., isolated from rotten biji (tofu residue)

Yi, Kwon Jung; Im, Wan Taek; Kim, Dong Ho; Kim, Soo Ki

doi:10.1099/ijsem.0.003009

“…The DNA G+C content fall within the range from 59.0 to 68.5 mol% [10]. At the time of writing, the genus Ottowia comprises seven identified species with validly published names [9][10][11][12][13][14][15]. Samples were collected from various environments, including activated sludge, rice beverage, fish intestines and tofu residue.…”

Section: Introductionmentioning

confidence: 99%

“…Common characteristics (Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, catalase- and oxidase-positive) are shared by the members of the genus Ottowia [11]. Ubiquinone 8 (Q-8) and C 16 : 0 , summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ) are the major quinone and major fatty acids, respectively [12]. The DNA G+C content fall within the range from 59.0 to 68.5 mol% [10].…”

Section: Introductionmentioning

confidence: 99%

Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge

Fa-hu

¹

,

Yang

²

,

Sun

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterium, designated BD-1T, was isolated from a sludge sample. Cells of the novel Gram-stain-negative strain were identified to be facultative anaerobic, non-motile and short rod-shaped. Growth occurred at 15–37 °C (optimum, 30 °C), pH 5.0–10.0 (pH 7.0) and in 0–4.0 % NaCl (2.0 %, w/v). The 16S rRNA gene sequence of strain BD-1T showed the highest sequence similarity to Ottowia thiooxydans DSM 14619T (97.0 %), followed by Ottowia pentelensis DSM 21699T (96.3 %) and less than 96 % to other related strains. The phylogenetic trees revealed that strain BD-1T clustered within the genus Ottowia . Summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c, 48.2 %), C16 : 0 (23.2 %) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c, 8.6 %) were the major fatty acids (>5 %), and ubiquinone-8 was the respiratory quinone. Phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylglycerol were identified as the major polar lipids. Meanwhile, the G+C content of the DNA was 63.6 mol% based on the draft genome analysis. The average nucleotide identity and digital DNA–DNA hybridization values between strain BD-1T and DSM 14619T were 74.5 and 21.4 %, respectively. In addition, the novel strain completely degraded 500 mg l−1 phenylacetic acid within 72 h under the condition of 3 % NaCl. Given the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain BD-1T was considered to represent a novel species of the genus Ottowia , for which the name Ottowia caeni sp. nov. is proposed. The strain is a potential resource for the bioremediation of phenylacetic acid contaminated water. The type strain is BD-1T (=CGMCC 1.18541T=KCTC 82183T).

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“…It was not detected in any background technical controls. Ottowia is a genus of bacteria that has been isolated from industrial and municipal wastewater (96–99), sikhye (100), tofu residue (101), and fish intestines (102); it has not been identified in investigations of the human uterus. Nevertheless, Ottowia is a member of the family Comamonadaceae, and Chen et al (23) and Winters et al (30) reported that Comamonadaceae was among the most relatively abundant bacterial taxa in the human endometrium.…”

Section: Discussionmentioning

confidence: 99%

Lack of evidence for microbiota in the placental and fetal tissues of rhesus macaques

Theis

¹

,

Romero

²

,

Winters

³

et al. 2020

Preprint

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ABSTRACTThe prevailing paradigm in obstetrics has been the sterile womb hypothesis. However, some are asserting that the placenta, intra-amniotic environment, and fetus harbor microbial communities. The objective of this study was to determine if the fetal and placental tissues of rhesus macaques harbor viable bacterial communities. Fetal, placental, and uterine wall samples were obtained from cesarean deliveries without labor (∼130/166 days gestation). The presence of viable bacteria in the fetal intestine and placenta was investigated through culture. The bacterial burden and profile of the placenta, umbilical cord, and fetal brain, heart, liver, and colon were determined through quantitative real-time PCR and DNA sequencing. These data were compared with those of the uterine wall, as well as to negative and positive technical controls. Bacterial cultures of fetal and placental tissues yielded only a single colony of Cutibacterium acnes. This bacterium was detected at a low relative abundance (0.02%) in the 16S rRNA gene profile of the villous tree sample from which it was cultured, yet it was also identified in 12/29 background technical controls. The bacterial burden and profile of fetal and placental tissues did not exceed or differ from those of background technical controls. In contrast, the bacterial burden and profiles of positive controls exceeded and differed from those of background controls. Among the macaque samples, distinct microbial signals were limited to the uterine wall. Therefore, using multiple modes of microbiologic inquiry, there was not consistent evidence of viable bacterial communities in the fetal and placental tissues of rhesus macaques.IMPORTANCEMicrobial invasion of the amniotic cavity (i.e. intra-amniotic infection) has been causally linked to pregnancy complications, especially preterm birth. Therefore, if the placenta and the fetus are typically populated by low biomass yet viable microbial communities, current understanding of the role of microbes in reproduction and pregnancy outcomes will need to be fundamentally reconsidered. Could these communities be of benefit by competitively excluding potential pathogens or priming the fetal immune system for the microbial bombardment it will experience upon delivery? If so, what properties (e.g. microbial load, community membership) of these microbial communities preclude versus promote intra-amniotic infection? Given the ramifications of the in utero colonization hypothesis, critical evaluation is required. In this study, using multiple modes of microbiologic inquiry (i.e. culture, qPCR, DNA sequencing) and controlling for potential background DNA contamination, we did not find consistent evidence for microbial communities in the placenta and fetal tissues of rhesus macaques.

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“…This species was named Ottowia thiooxidans . Meanwhile, six additional species of the genus and one species without a validated name have been proposed, namely Ottowia beijingensis [2], Ottowia caeni [3], Ottowia flava [4], Ottowia konkukae [5], Ottowia oryzae [6], Ottowia pentelensis [7] and ‘ Ottowia shaoguanensis ’ [8]. The genus Ottowia is classified within the family Comamonadaceae and the phylum Betaproteobacteria .…”

Section: Full-textmentioning

confidence: 99%

“…Data for strain 1 from this study; data for strains 2 and 3 from Shi et al[3], obtained with identical methods and precultivation conditions. Data for strains 2 (in brackets), 4, 5 and 7 were obtained by Heo et al[5]; data for strain 6 from Yi et al[4]. tr, trace amounts (<0.5 %).…”

mentioning

confidence: 99%

Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis)

Busse¹,

Kämpfer

²

,

Szostak³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain designated 27CT isolated from the cloaca of a giant Asian pond turtle was subjected to polyphasic taxonomic characterization. The strain was Gram-stain negative and oxidase- and catalase-positive. It had highest 16S rRNA gene sequence similarity to Ottowia beijingensis GCS-AN-3T (97.6 %) and Ottowia flava GY511T 96.0% and less than 96.0 % to other established species including Ramlibacter rhizophilus YS3.2.7T, Ottowia konkukae SK3863T, Acidovorax caeni E-24608T and Ottowia thiooxydans DSM 14619T. Phylogenetically, strain 27CT formed a branch with O. beijingensis GCS-AN-3T within the Ottowia clade. The genome size was 4.32 Mbp and the G+C content was 65.7 mol%. Strain 27CT shared highest ANIb values with O. beijingensis GCS-AN-3T (82.71/82.73 %) followed by O. oryzae KADR8-3T (78.9/79.0 %) and O. caeni BD-1T (73.3/75.2 %). The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid and the quinone system was ubiquinone Q-8. Predominant compounds in the polar lipid profile were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. Major polyamines were 2-hydroxyputrescine and putrescine. In the fatty acid profile, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C16 : 0, summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C14 : 0, C10 : 0 3-OH and C16 : 0 2-OH were detected. All these data identify strain 27CT as representing a novel species of the genus Ottowia and hence we propose the name Ottowia testudinis sp. nov. The type strain is 27CT (=CCM 9138T=LMG 32213T).

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Ottowia konkukae sp. nov., isolated from rotten biji (tofu residue)

Cited by 13 publications

References 15 publications

Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge

Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge

Lack of evidence for microbiota in the placental and fetal tissues of rhesus macaques

Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis)

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