OTS1 and OTS2 SUMO proteases link plant development and survival under salt stress

Conti, Lucio; Kioumourtzoglou, Dimitrios; O’Donnell, Elizabeth; Dominy, Peter; Sadanandom, Ari

doi:10.4161/psb.4.3.7867

Cited by 24 publications

(16 citation statements)

References 12 publications

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“…HMWC in the E3 ligase mutant siz1 were considerably loss-of-function generates leaf growth defects and late germination, also confirming previous evidence for the existence of an early flowering phenotype (Conti et al 2008). I n previous studies, overexpression of ULP1c/d already suggested a role for these proteases in rosette development (Conti et al 2009), and, more recently, ULP1c/d were shown to prevent accumulation of sumoylated DELLA proteins, which themselves lead to growth restraint (Conti et al 2014). Our current phenotypes are consistent with a model in which ulp1c/d growth defects can be attributed to SUMO-dependent stabilization of DELLAs (Fig.…”

Section: The Ulp1c/d Mutant Displays Altered Sumo-conjugate Levelssupporting

confidence: 74%

SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana

et al. 2016

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Sumoylation is an essential post-translational regulator of plant development and the response to environmental stimuli. SUMO conjugation occurs via an E1-E2-E3 cascade, and can be removed by SUMO proteases (ULPs). ULPs are numerous and likely to function as sources of specificity within the pathway, yet most ULPs remain functionally unresolved. In this report we used loss-of-function reverse genetics and transcriptomics to functionally characterize Arabidopsis thaliana ULP1c and ULP1d SUMO proteases. GUS reporter assays implicated ULP1c/d in various developmental stages, and subsequent defects in growth and germination were uncovered using loss-of-function mutants. Microarray analysis evidenced not only a deregulation of genes involved in development, but also in genes controlled by various drought-associated transcriptional regulators. We demonstrated that ulp1c ulp1d displayed diminished in vitro root growth under low water potential and higher stomatal aperture, yet leaf transpirational water loss and whole drought tolerance were not significantly altered. Generation of a triple siz1 ulp1c ulp1d mutant suggests that ULP1c/d and the SUMO E3 ligase SIZ1 may display separate functions in development yet operate epistatically in response to water deficit. We provide experimental evidence that Arabidopsis ULP1c and ULP1d proteases act redundantly as positive regulators of growth, and operate mainly as isopeptidases downstream of SIZ1 in the control of water deficit responses.

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Section: The Ulp1c/d Mutant Displays Altered Sumo-conjugate Levelssupporting

confidence: 74%

SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana

et al. 2016

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“…Similar to the ubiquitylation system, sumoylation is processed through the SUMO-specific E1, E2 and E3 enzymes, and SUMO is covalently conjugated to the target consensus motif ΨKxE/D (Ψ, hydrophobic amino acid; K, SUMO target lysine; x, any amino acid; E/D, glutamic acid/aspartic acid). Both loss- and gain-of-function analyses reveal that sumoylation functions in the regulation of responses to abiotic and biotic stresses [134,135,137], such as the response to nutrient availability [138–140], drought tolerance [141,142], basal thermotolerance [143], salt stress tolerance [144,145], copper tolerance [146] and innate immunity [147,148], as well as the development [149–153] and regulation of ABA signaling [154,155]. As the siz1 mutant, which is impaired in the SUMO E3 ligase [138], exhibits hypersensitivity to chilling and freezing stresses [26], sumoylation contributes to the regulation of cold signaling through the stabilization of ICE1.…”

Section: Post-translational Regulationmentioning

confidence: 99%

Cold Signaling and Cold Response in Plants

Miura

Furumoto

2013

IJMS

395

295

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Plants are constantly exposed to a variety of environmental stresses. Freezing or extremely low temperature constitutes a key factor influencing plant growth, development and crop productivity. Plants have evolved a mechanism to enhance tolerance to freezing during exposure to periods of low, but non-freezing temperatures. This phenomenon is called cold acclimation. During cold acclimation, plants develop several mechanisms to minimize potential damages caused by low temperature. Cold response is highly complex process that involves an array of physiological and biochemical modifications. Furthermore, alterations of the expression patterns of many genes, proteins and metabolites in response to cold stress have been reported. Recent studies demonstrate that post-transcriptional and post-translational regulations play a role in the regulation of cold signaling. In this review article, recent advances in cold stress signaling and tolerance are highlighted.

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“…They are together essential for normal embryogenesis (Saracco et al ., ) and also jointly suppress salicylic acid (SA)‐dependent defence responses (van den Burg et al ., ; Cheong et al ., ). SUMO proteases that catalyse deconjugation to recycle free SUMO have been shown to be implicated in multiple regulatory pathways critical for normal plant development (Colby et al ., ; Hermkes et al ., ; Murtas et al ., ; Reeves et al ., ; Xu et al ., ) and plant–pathogen interaction for altering host cell defence (Roden et al ., ) as well as plant slat tolerance (Conti et al ., , ).…”

Section: Introductionmentioning

confidence: 98%

Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass

Zhou

et al. 2012

Plant Biotechnology Journal

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SummarySumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to environmental stress in plants. To study the feasibility of manipulating SUMO E3 ligase, one of the important components in the sumoylation pathway in transgenic (TG) crop plants for improving overall plant performance under adverse environmental conditions, we have analysed TG creeping bentgrass (Agrostis stolonifera L.) plants constitutively expressing OsSIZ1, a rice SUMO E3 ligase. Overexpression of OsSIZ1 led to increased photosynthesis and overall plant growth. When subjected to water deficiency and heat stress, OsSIZ1 plants exhibited drastically enhanced performance associated with more robust root growth, higher water retention and cell membrane integrity than wild-type (WT) controls. OsSIZ1 plants also displayed significantly better growth than WT controls under phosphatestarvation conditions, which was associated with a higher uptake of phosphate (Pi) and other minerals, such as potassium and zinc. Further analysis revealed that overexpression of OsSIZ1 enhanced stress-induced SUMO conjugation to substrate in TG plants, which was associated with modified expression of stress-related genes. This strongly supports a role sumoylation plays in regulating multiple molecular pathways involved in plant stress response, establishing a direct link between sumoylation and plant response to environmental adversities. Our results demonstrate the great potential of genetic manipulation of sumoylation process in TG crop species for improved resistance to broad abiotic stresses.

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OTS1 and OTS2 SUMO proteases link plant development and survival under salt stress

Cited by 24 publications

References 12 publications

SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana

SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana

Cold Signaling and Cold Response in Plants

Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass

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