Otolin-1, an otolith- and otoconia-related protein, controls calcium carbonate bioinspired mineralization

Bielak, Klaudia; Benkowska-Biernacka, Dominika; Ptak, Maciej; Stolarski, Jarosław; Kalka, Marta; Ożyhar, Andrzej; Dobryszycki, Piotr

doi:10.1016/j.bbagen.2023.130327

Cited by 4 publications

(8 citation statements)

References 39 publications

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“…In contrast to prestin, which is instrumental in the amplification of auditory signals, otolin-1 plays a pivotal role in the vestibular system, which is integral to the maintenance of balance and spatial orientation [ 16 ]. Specifically, otolin-1 is a component of otoconia - the calcium carbonate crystals found within the utricle and saccule of the inner ear [ 17 ]. These otolith organs play a critical role in detecting gravity and linear acceleration.…”

Section: Discussionmentioning

confidence: 99%

“…Otolin-1 represents another promising inner ear biomarker [ 16 ]. This collagen-like protein functions as a scaffold enabling calcium carbonate deposition into otoconia [ 17 ]. Otolin-1 is expressed in the vestibular maculae and cristae, as well as within the tectorial membrane, the marginal cells of the stria vascularis, and Claudius cells [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Serum Prestin and Otolin-1 Levels in Pilots of Helicopter-Based Emergency Medical Services: Potential Markers for Ear Injury

Minoretti,

Santiago Sáez,

Liaño Riera

et al. 2024

Cureus

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Introduction: Occupational noise exposure is a major public health concern, impacting a large workforce worldwide. In this study, we sought to evaluate the serum concentrations of prestin, a cochlear protein that diminishes following noise exposure, and otolin-1, a protein secreted into the bloodstream subsequent to inner ear damage, among three diverse professional categories, each exposed to varying degrees of noise. Helicopter emergency medical service (HEMS) pilots and construction workers were considered high-risk groups due to their elevated exposure to occupational noise, whereas office workers were regarded as a low-risk group, reflecting their comparatively minimal noise exposure. Methods: The study sample included 60 males, encompassing helicopter pilots, construction laborers, and office workers (n=20, each). Recruitment occurred during standard occupational health visits, with all participants presenting normal clinical audiograms. Serum levels of prestin and otolin-1 were measured in duplicate using commercially available immunoassays and compared across the three professional categories. Results: HEMS pilots had the lowest mean serum prestin level at 211±27 pg/mL, followed by construction workers at 234±29 pg/mL, and office workers at 269±42 pg/mL (p<0.001, one-way analysis of variance), with all inter-group differences statistically significant (p<0.05, Tukey's post hoc tests). For otolin-1, HEMS pilots showed the highest mean at 216±20 pg/mL, with construction workers at 196±22 pg/mL, and office workers at 181±20 pg/mL (p<0.001, one-way analysis of variance). Statistically significant differences were found between HEMS pilots and both other groups for otolin-1 levels (p<0.05, Tukey's post hoc tests), but not between construction workers and office workers. Conclusions: Serum concentrations of prestin and otolin-1 may differ among healthy individuals according to their occupational noise exposure and have the potential to act as indicators of subclinical inner ear injury. To substantiate these preliminary observations, incorporating exposure assessment, especially via direct measurements of noise and vibration exposure, would markedly improve the reliability of our findings.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serum Prestin and Otolin-1 Levels in Pilots of Helicopter-Based Emergency Medical Services: Potential Markers for Ear Injury

Minoretti,

Santiago Sáez,

Liaño Riera

et al. 2024

Cureus

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“…This otolithic membrane must expand outwards to support the perennial growth of the otolith, yet remain porous to allow endolymph K + ions to reach the HCs at the center of the macula. Some SC also appears to express Otolin-1 in the middle of its apical membrane, which suggests it may be contributing to the biomineralization of the otolith near the central region of the macula (30–32). Interestingly, previous Otolin-1 immunostaining on the chum salmon ( Oncorhychus keta ) and the rainbow trout ( Oncorhynchus mykiss ) were immunonegative (31, 43).…”

Section: Discussionmentioning

confidence: 99%

“…Similar to previous studies (31, 43), Otolin-1 presence is significantly greater within the GC than in any other inner ear cell type. This suggests the GC is not only critical to otolith biomineralization, but also necessary for proper CaCO 3 arrangement: calcite has a rhombohedral crystal structure and is formed in the presence of exclusively Otolin-1, whereas aragonite has an orthorhombic crystal structure formation that requires the presence of both Otolin-1 and otolith matrix molecule 64 (OMM-64) during in vitro trials (30, 68). The abundant presence of Otolin-1 in the GC around the macula also matches the natural biomineralization patterns: the proximal side (facing the macula) is known to grow faster and have wider increments than the distal side (facing the patches area) of the otolith (40).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical and ultrastructural characterization of the inner ear epithelial cells of splitnose rockfish (Sebastes diploproa)

Kwan,

Andrade,

Prime

et al. 2023

Preprint

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The inner ear of teleost fish regulates the ionic and acid-base chemistry and secretes the protein matrix of the endolymph to facilitate otolith biomineralization, which are used to maintain vestibular and auditory functions. The otolith is biomineralized in a concentric ring pattern corresponding to seasonal growth, and this CaCO3 polycrystal has become a vital aging and life-history tool for fishery managers, ecologists, and conservation biologists. Moreover, biomineralization patterns are sensitive to environmental variability including climate change, thereby threatening the accuracy and relevance of otolith-reliant toolkits. However, the cellular biology of the inner ear is poorly characterized, which is a hurdle for a mechanistic understanding of the underlying processes. This study provides a systematic characterization of the cell types in the inner ear of splitnose rockfish (Sebastes diploproa). Scanning electron microscopy revealed the apical morphologies of the six inner ear cell types. Additionally, immunostaining and confocal microscopy characterized the expression and subcellular localization of the proteins Na+/K+-ATPase, carbonic anhydrase, V-type H+-ATPase, Na+-K+-2Cl--Co-Transporter, Otolith Matrix Protein 1, and Otolin-1 in six inner ear cell types bordering the endolymph. This fundamental cytological characterization of the rockfish inner ear epithelium illustrates the intricate physiological processes involved in otolith biomineralization, and highlights how greater mechanistic understanding is necessary to predict their multi-stressor responses to future climate change.

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“…The surrounding SCs are thought to aid in secreting the matrix proteins, and anchoring the filamentous material with cell boundary microvilli as proposed for inner ear systems of aves and amphibians (20,28,29). The GC has an extensive endoplasmic reticulum and numerous secretion granules (18) to synthesize and secrete proteins such as Otolin-1 to chelate with Ca 2+ ions to promote biomineralization (30)(31)(32)(33)(34)(35). The meshwork area is known to have T1-Is and T2-Is (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical and ultrastructural characterization of the inner ear epithelial cells of splitnose rockfish (Sebastes diploproa)

Kwan,

Andrade,

Prime

et al. 2024

American Journal of Physiology-Regulatory, Integrative and Comp

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The inner ear of teleost fish regulates the ionic and acid-base chemistry and secretes the protein matrix of the endolymph to facilitate otolith biomineralization, which are used to maintain vestibular and auditory functions. The otolith is biomineralized in a concentric ring pattern corresponding to seasonal growth, and this CaCO3polycrystal has become a vital aging and life-history tool for fishery managers, ecologists, and conservation biologists. Moreover, biomineralization patterns are sensitive to environmental variability including climate change, thereby threatening the accuracy and relevance of otolith-reliant toolkits. However, the cellular biology of the inner ear is poorly characterized, which is a hurdle for a mechanistic understanding of the underlying processes. This study provides a systematic characterization of the cell types in the inner ear of splitnose rockfish ( Sebastes diploproa). Scanning electron microscopy revealed the apical morphologies of the six inner ear cell types. Additionally, immunostaining and confocal microscopy characterized the expression and subcellular localization of the proteins Na+/K+-ATPase, carbonic anhydrase, V-type H+-ATPase, Na+-K+-2Cl--Co-Transporter, Otolith Matrix Protein 1, and Otolin-1 in six inner ear cell types bordering the endolymph. This fundamental cytological characterization of the rockfish inner ear epithelium illustrates the intricate physiological processes involved in otolith biomineralization, and highlights how greater mechanistic understanding is necessary to predict their multi-stressor responses to future climate change.

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Otolin-1, an otolith- and otoconia-related protein, controls calcium carbonate bioinspired mineralization

Cited by 4 publications

References 39 publications

Serum Prestin and Otolin-1 Levels in Pilots of Helicopter-Based Emergency Medical Services: Potential Markers for Ear Injury

Serum Prestin and Otolin-1 Levels in Pilots of Helicopter-Based Emergency Medical Services: Potential Markers for Ear Injury

Immunohistochemical and ultrastructural characterization of the inner ear epithelial cells of splitnose rockfish (Sebastes diploproa)

Immunohistochemical and ultrastructural characterization of the inner ear epithelial cells of splitnose rockfish (Sebastes diploproa)

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