Oto-endoscopy: A reliable and validated technique for phenotyping otitis media in the mouse

Bhutta, Mahmood F.; Hedge, Elizabeth A.; Parker, Andrew; Cheeseman, Michael; Brown, S. D. M.

doi:10.1016/j.heares.2010.09.002

Cited by 3 publications

(6 citation statements)

References 36 publications

(29 reference statements)

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“…This supports use of this device to reliably screen and evaluate ear disease, in particular in remote or rural locations in low‐ or middle‐income countries. We also found that video capture was preferable to capturing still images as it provided depth perception, a finding reported in other studies …”

Section: Discussionsupporting

confidence: 82%

“…We also found that video capture was preferable to capturing still images as it provided depth perception, a finding reported in other studies. 16,17 When comparing the Cupris© device to standard assessment, the same diagnosis was reached in 95% of ears. The Cohen's kappa coefficient of 0.89 also evidences high inter-rater agreement in primary diagnosis.…”

Section: Synopsis Of Findings and Clinical Applicabilitymentioning

confidence: 83%

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A cross‐sectional evaluation of the validity of a smartphone otoscopy device in screening for ear disease in Nepal

Mandavia

Lapa

Smith³

et al. 2017

Clinical Otolaryngology

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Objectives Hearing loss is a neglected international health problem. The greatest burden of ear disease is in low‐income countries where there is also a lack of resources. In this context, screening for otological disease may be worthwhile. Cupris© has developed an otoscopy device that offers the possibility of low‐cost mass screening in remote communities. We evaluated the validity of this device in diagnosing ear disease and in determining whether referral to an ENT centre is warranted. Design Cross‐sectional study. Setting Outpatient clinic, Nepal. Participants All adults and children were invited to take part over a 2‐day period. The Cupris© device was used to record participants otological history and examination. Stored history and images were assessed in the United Kingdom by a Consultant‐grade ENT Surgeon, who provided a diagnosis and decided whether referral to an ENT centre was warranted. After screening with the Cupris© device, participants were immediately assessed by a UK trained ENT Consultant Surgeon using a standard otoscope (“standard assessment”). A diagnosis was recorded for each participant and a decision was made as to whether referral to an ENT centre was warranted. Outcomes Concordance in primary diagnosis (analysed per ear) and concordance in the decision to refer (analysed per patient). Cohen's kappa coefficient for inter‐rater agreement in diagnosis. Results Fifty‐six patients agreed to participate. In four patients, the quality of video recorded precluded a diagnosis or management plan. These patients were excluded from subsequent analysis, leaving 52 patients for analysis. The same diagnosis was reached for 99 of 104 ears when comparing the Cupris© device to standard assessment (95% concordance), with Cohen's kappa coefficient of 0.89. The decision as to whether a patient should be referred to an ENT centre for further assessment was the same for all 52 participants when comparing the Cupris© device to standard assessment. Conclusions When compared to standard assessment, the Cupris© device is a valid tool for the diagnosis of ear disease and decision for onward referral. It shows considerable promise for use by trained non‐medical workers, as a low‐cost and portable tool to screen for ear disease in remote settings, particularly in low‐ and middle‐income countries.

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Section: Discussionsupporting

confidence: 82%

Section: Synopsis Of Findings and Clinical Applicabilitymentioning

confidence: 83%

A cross‐sectional evaluation of the validity of a smartphone otoscopy device in screening for ear disease in Nepal

Mandavia

Lapa

Smith³

et al. 2017

Clinical Otolaryngology

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“…The right ear was left unoperated to act as a control. Prior to operation, all Junbo mice were assessed for bilateral visible evidence of tympanic membrane opacity, which we have previously shown to be a reliable marker of inflammatory effusion in the murine middle ear …”

Section: Methodsmentioning

confidence: 99%

“…Prior to operation, all Junbo mice were assessed for bilateral visible evidence of tympanic membrane opacity, which we have previously shown to be a reliable marker of inflammatory effusion in the murine middle ear. 24 At intervals after myringotomy groups of mice were euthanized and skinned heads were fixed for 48 hours in 10% neutral buffered formalin, decalcified with Immunocal (Decal Chemical Corp., Tallman, NY) for 72 hours, and embedded in paraffin wax. Three-micrometer sections were stained with hematoxylin and eosin.…”

Section: Myringotomy In Three Mouse Cohortsmentioning

confidence: 99%

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Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

2014

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Objectives/Hypothesis: Ventilation of the chronically inflamed middle ear is a key outcome in functional middle ear surgery. Grommets eliminate middle ear effusion, but there is also evidence that they downregulate inflammation. The reason for this is not understood, but there is little to suggest alteration in eustachian tube ventilatory capacity. Previous work has shown that the Junbo mouse model of chronic otitis media has hypoxic middle ear mucosa and bulla fluid leucocytes. Here we explore whether surgical ventilation may alleviate chronic otitis media through downregulation of hypoxia.Study Design: Surgical intervention on a mouse model of disease. Methods: We established patency of myringotomy incision as 5 days in wild-type mice. We performed unilateral myringotomy on three cohorts of mice: 10 wild-type controls, 12 Junbo mice, and 15 Junbo mice with additional removal of middle ear effusion. A small cohort of these mice were labeled in vivo by intraperitoneal injection of pimonidazole to identify tissue hypoxia. Tissues were assessed for mucoperiosteal thickening and pimonidazole labeling, comparing operated to nonoperated ears.Results: Ventilation of the inflamed Junbo middle ear revealed significant reduction in inflammatory thickening associated with loss of pimonidazole labeling, suggesting resolution of cellular hypoxia.Conclusions: Surgical ventilation may achieve therapeutic effect through alleviation of cellular hypoxia in the chronically inflamed middle ear. Targeted molecular therapy of hypoxia signaling may offer future alternative therapy for chronic otitis media.

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Smartphone otoscopy by non-specialist health workers in rural Greenland: A cross-sectional study

Demant

Jensen

Bhutta³

et al. 2019

International Journal of Pediatric Otorhinolaryngology

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Oto-endoscopy: A reliable and validated technique for phenotyping otitis media in the mouse

Cited by 3 publications

References 36 publications

A cross‐sectional evaluation of the validity of a smartphone otoscopy device in screening for ear disease in Nepal

A cross‐sectional evaluation of the validity of a smartphone otoscopy device in screening for ear disease in Nepal

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

Smartphone otoscopy by non-specialist health workers in rural Greenland: A cross-sectional study

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