Otitis media with effusion is not a sterile inflammatory process: scanning electron microscope evidence

Elzayat, Saad; Nosair, Nahla; Ghazy, Amany A.; Abdulghaffar, Ibrahim A

doi:10.1007/s00405-020-06338-4

Cited by 11 publications

(11 citation statements)

References 25 publications

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“…S. pneumoniae biofilms have been frequently found in the middle ear mucosa of children with OM. ,, So, the activity of encapsulated MSlys against biofilms was evaluated (Figure ). Both MSlys and MSlys-loaded liposomes significantly reduced the number of pneumococcal cells from 24 h biofilms in all the time points tested ( P < 0.05).…”

Section: Results and Discussionmentioning

confidence: 99%

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

et al. 2021

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Local delivery of antimicrobials for otitis media treatment would maximize therapeutic efficacy while minimizing side effects. However, drug transport across the tympanic membrane in the absence of a delivery system is challenging. In this study, the MSlys endolysin was encapsulated in deformable liposomes for a targeted treatment of S. pneumoniae, one of the most important causative agents of otitis media. MSlys was successfully encapsulated in liposomes composed of l-alpha-lecithin and sodium cholate (5:1) or l-alpha-lecithin and PEG2000 PE (10:1), with encapsulation efficiencies of about 35%. The PEGylated and sodium cholate liposomes showed, respectively, mean hydrodynamic diameters of 85 and 115 nm and polydispersity indices of 0.32 and 0.42, both being stable after storage at 4 °C for at least one year. Both liposomal formulations showed a sustained release of MSlys over 7 days. Cytotoxicity studies against fibroblast and keratinocyte cell lines revealed the biocompatible nature of both MSlys and MSlys-loaded liposomes. Additionally, the encapsulated MSlys showed prompt antipneumococcal activity against planktonic and biofilm S. pneumoniae, thus holding great potential for transtympanic treatment against S. pneumoniae otitis media.

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Section: Results and Discussionmentioning

confidence: 99%

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

et al. 2021

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“…The presence of Alloiococcus otitidis has been considered as a precipitating factor of OME [ 46 ]. Likewise, the importance of “bacterial biofilm” and its association with persistent otic disease has been observed [ 47 , 48 , 49 ]. The local administration of probiotic bacteria seems to have the ability to inhibit the growth of otopathogens [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of the Traditional Mediterranean Diet in Patients with Otitis Media with Effusion

Calatayud-Sáez¹,

Calatayud²,

Calatayud³

2021

Nutrients

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Introduction: Otitis media with effusion (OME) is common in pediatric primary care consultations. Its etiology is multifactorial, although it has been proven that inflammation factors mediate and that immunity is in a phase of relative immaturity. The objective of this study was to assess the effects of the Traditional Mediterranean Diet (TMD) modulating inflammation and immunity in patients diagnosed with OME. Materials and Methods: A analysis as a single-group pre-test/post-test was conducted on 40 girls and 40 boys between 18 months and 5 years old. Tympanometry normalization was the main test to control the benefit of diet. Clinical and therapeutic variables were studied through evaluation questionnaires, a quality test of the diet, as well as various anthropometric parameters. Results: At the end of one year, tympanometry had normalized in 85% of patients. The remaining 15% had normal audiometry and/or associated symptoms had decreased. Likewise, episodes of recurrent colds decreased from 5.96 ± 1.41 to 2.55 ± 0.37; bacterial complications of 3.09 ± 0.75 to 0.61 ± 0.06 and persistent nasal obstruction of 1.92 ± 0.27 to 0.26 ± 0.05. The degree of satisfaction of the families with the program was very high. Conclusions: The application of the Traditional Mediterranean Diet could have promising effects in the prevention and treatment of otitis media with effusion.

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“…Following title and abstract screening, 19 full-text articles were retrieved and screened for eligibility. Of them, 11 articles were excluded, and eight studies [11][12][13][14][15][16][17][18] (n = 402 patients, n = 504 samples) were reviewed in detail and included in this meta-analysis…”

Section: Search Results and Characteristics Of Included Studiesmentioning

confidence: 99%

The Prevalence and Association of Biofilms With Otitis Media With Effusion: A Systematic Review and Meta-Analysis

Elzayat,

El-Deeb,

El-Shirbeny

et al. 2023

Ann Otol Rhinol Laryngol

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Purpose: We aimed to identify the role of bacterial biofilms in the chronicity of otitis media with effusion and its resistance to antibiotics. We illustrated this role by reviewing, analyzing, and correlating the findings with the results of the included studies to reach clear evidence. Methods: A comprehensive search of electronic databases (Scopus, PubMed, Web of Science, Cochrane, and GHL databases) was performed for all studies using the following strategy till April 2021 with the search terms: Biofilm and Middle ear effusion. We found 935 references, 421 were duplicates, and 514 were needed for further screening, and it was as follows: PubMed 215, Scopus 18, Cochrane 130, Web of Science 136, and GHL 15. Results: The pooled prevalence of culture-positive effusions was estimated to be 40% (95% CI [28%, 53%]) of the total OME population. Overall, the prevalence of PCR-positive effusions was estimated to be 97% (95% CI [95%, 99%]) of the total OME population. The pooled prevalence of EM-positive effusions was estimated to be 82% (95% CI [69%, 95%]) of the total OME population. Conclusion: The data presented in this study coincide with the significant role of bacterial biofilms in the pathogenesis of chronic otitis media with effusion. The involvement of bacterial biofilm as a component of the OME pathogenic process can help us to explain why antimicrobial therapy is not always effective in the eradication of the disease process and, also explain the recurrence of middle ear effusion after treatment with tympanostomy tubes either with or without adenoidectomy.

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Otitis media with effusion is not a sterile inflammatory process: scanning electron microscope evidence

Cited by 11 publications

References 25 publications

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment

Effects of the Traditional Mediterranean Diet in Patients with Otitis Media with Effusion

The Prevalence and Association of Biofilms With Otitis Media With Effusion: A Systematic Review and Meta-Analysis

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