Other spoilage bacteria

Betts, Gail

doi:10.1533/9781845691417.5.668

Cited by 18 publications

(5 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acinetobacter , belonging to the Moraxellaceae family, was detected on most chicken samples despite the observed differences in detection abundances. Although is a strictly aerobic, Gram-negative microorganism, its occurrence on fresh and spoiled meats under aerobic, modified atmosphere and vacuum-storage conditions is well documented [ 14 , 37 , 42 , 61 , 86 , 87 , 88 , 89 ]. The proportion of Acinetobacter decreased on all chicken samples stored at 0 °C and 5 °C or remained stable on thigh samples stored at 10 °C.…”

Section: Discussionmentioning

confidence: 99%

“…The proportion of Acinetobacter decreased on all chicken samples stored at 0 °C and 5 °C or remained stable on thigh samples stored at 10 °C. This could be attributed to the relatively high optimal growth temperature of Acinetobacter (i.e., from 20 °C up to 44 °C, depending on strain) [ 86 , 90 , 91 ]. Psychrobacter , also belonging to the Moraxellaceae family, was detected as a small part of the initial microbiota.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microbiota of Chicken Breast and Thigh Fillets Stored under Different Refrigeration Temperatures Assessed by Next-Generation Sequencing

Dourou¹,

Spyrelli

Doulgeraki³

et al. 2021

Foods

View full text Add to dashboard Cite

Chicken is one of the most widely consumed meats worldwide. The exploration of the bacterial diversity of chicken meat may provide new insights into the chicken-associated microbiome that will lead to moderation of food spoilage or safety. This study was undertaken to explore the bacterial communities of chicken breast and thigh fillets stored at refrigeration (0 °C and 5 °C) and slightly abuse (10 °C) temperatures for 5 days through conventional cultural methods along with next-generation sequencing (NGS) analysis. Total viable counts (TVC), Brochothrix thermosphacta, Pseudomonas spp., and lactic acid bacteria (LAB) were enumerated, while the bacterial communities were mapped through 16S rRNA gene amplicon sequencing. Chicken breast and thigh fillets possessed a complex bacterial structure that incorporated a total of >200 Operational Taxonomic Units (OTUs) at the genus level. The core microbiota of fresh samples consisted of Acinetobacter, Brochothrix, Flavobacterium, Pseudomonas, Psychrobacter, and Vibrionaceae (family). These genera persisted until the end of storage in >80% of samples, except Psychrobacter and Flavobacterium, while Photobacterium was also identified. Hierarchical clustering showed a distinction of samples based on storage time and chicken part. Conventional plate counting with growth media commonly used in spoilage studies did not always correspond to the microbial community profiles derived from NGS analysis, especially in Pseudomonas, Acinetobacter, Photobacterium, and Vibrionaceae. Results of the present study highlight Photobacterium and Vibrionaceae, in general, as potent chicken meat spoilers and suggest the necessity to combine classical microbiological methods along with NGS technologies to characterize chicken meat spoilage microbiota.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Microbiota of Chicken Breast and Thigh Fillets Stored under Different Refrigeration Temperatures Assessed by Next-Generation Sequencing

Dourou¹,

Spyrelli

Doulgeraki³

et al. 2021

Foods

View full text Add to dashboard Cite

show abstract

“…Among these, Flavobacteria are known to include commensal and opportunistic pathogens (Crump et al, 2001). Though a few species of Flavobacterium may grow weakly under micro- and anaerobic conditions, many species are obligately aerobic and occurring in a variety of terrestrial and aquatic environments, and food (Betts, 2006). Other typical taxa at the surface included genera potentially associated with human and animal hosts (e.g., Roseomonas and Lautropia ), as well as aerobic, anoxygenic (such as Sandarakinorhabdus ; Gich and Overmann, 2006) and oxygenic (Cyanobacteria; Whitton, 2012) phototrophic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Effects of Habitat Partitioning on the Distribution of Bacterioplankton in Deep Lakes

Salmaso

2019

Front. Microbiol.

View full text Add to dashboard Cite

In deep lakes, many investigations highlighted the existence of exclusive groups of bacteria adapted to deep oxygenated and hypoxic and anoxic hypolimnia. Nevertheless, the extent of bacterial strain diversity has been much less scrutinized. This aspect is essential for an unbiased estimation of genetic variation, biodiversity, and population structure, which are essential for studying important research questions such as biogeographical patterns, temporal and spatial variability and the environmental factors affecting this variability. This study investigated the bacterioplankton community in the epilimnetic layers and in the oxygenated and hypoxic/anoxic hypolimnia of five large and deep lakes located at the southern border of the Alps using high throughput sequencing (HTS) analyses (16S rDNA) and identification of amplicon sequence variants (ASVs) resolving reads differing by as little as one nucleotide. The study sites, which included two oligomictic (Garda and Como) and three meromictic lakes (Iseo, Lugano, and Idro) with maximum depths spanning from 124 to 410 m, were chosen among large lakes to represent an oxic-hypoxic gradient. The analyses showed the existence of several unique ASVs in the three layers of the five lakes. In the case of cyanobacteria, this confirmed previous analyses made at the level of strains or based on oligotyping methods. As expected, the communities in the hypoxic/anoxic monimolimnia showed a strong differentiation from the oxygenated layer, with the exclusive presence in single lakes of several unique ASVs. In the meromictic lakes, results supported the hypothesis that the formation of isolated monimolimnia sustained the development of highly diversified bacterial communities through ecological selection, leading to the establishment of distinctive biodiversity zones. The genera identified in these layers are well-known to activate a wide range of redox reactions at low O2 conditions. As inferred from 16S rDNA data, the highly diversified and coupled processes sustained by the monimolimnetic microbiota are essential ecosystem services that enhance mineralization of organic matter and formation of reduced compounds, and also abatement of undesirable greenhouse gasses.

show abstract

“…biofilm formation often occurs, especially under conditions of reduced aeration [62]. According to the amplicon sequencing, we have shown that the leaf litter consortium BL6 contains both aerobes (Achromobacter) [63], microaerophiles (Methyloversatilis, Magnetospirillum) [64,65], and strict anaerobes (Ruminiclostridium, Anaerocolumna, Herbinix) [66][67][68], which can be a sign of biofilm formation in this consortium, since they are often formed in nature during the breakdown of lignocellulose [69]. In the consortium OS4, we observed Flavobacterium as the major component, which also was reported to be able to form biofilms [70].…”

Section: Diversity Of Members Of the Cellulolytic Consortiamentioning

confidence: 97%

The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates

Gladkov

Kimeklis

Afonin

et al. 2022

IJMS

View full text Add to dashboard Cite

Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation. Although many bacterial strains from natural substrates demonstrate cellulolytic activities, the CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse and usually distributed among different bacteria in one habitat. Thus, using microbial consortia can be a solution to rapid and effective decomposition of plant biomass. Four cellulolytic consortia were isolated from enrichment cultures from composting natural lignocellulosic substrates—oat straw, pine sawdust, and birch leaf litter. Enrichment cultures facilitated growth of similar, but not identical cellulose-decomposing bacteria from different substrates. Major components in all consortia were from Proteobacteria, Actinobacteriota and Bacteroidota, but some were specific for different substrates—Verrucomicrobiota and Myxococcota from straw, Planctomycetota from sawdust and Firmicutes from leaf litter. While most members of the consortia were involved in the lignocellulose degradation, some demonstrated additional metabolic activities. Consortia did not differ in the composition of CAZymes genes, but rather in axillary functions, such as ABC-transporters and two-component systems, usually taxon-specific and associated with CAZymes. Our findings show that enrichment cultures can provide reproducible cellulolytic consortia from various lignocellulosic substrates, the stability of which is ensured by tight microbial relations between its components.

show abstract

Other spoilage bacteria

Cited by 18 publications

References 36 publications

Microbiota of Chicken Breast and Thigh Fillets Stored under Different Refrigeration Temperatures Assessed by Next-Generation Sequencing

Microbiota of Chicken Breast and Thigh Fillets Stored under Different Refrigeration Temperatures Assessed by Next-Generation Sequencing

Effects of Habitat Partitioning on the Distribution of Bacterioplankton in Deep Lakes

The Structure of Stable Cellulolytic Consortia Isolated from Natural Lignocellulosic Substrates

Contact Info

Product

Resources

About