Other HPLC separations performed on hydrophobic stationary phases

Moldoveanu, Serban C.; David, Víctor

doi:10.1016/b978-0-323-91177-1.00013-2

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…The majority of LC applications currently exploit reliable and reproducible separation of non-polar compounds through reversed-phase LC [22]. However, since a significant part of metabolites bear polar or ionic group, their separation by reversed-phase liquid chromatography often requires chemical modification through derivatization or relies on ion pairing [23,24]. Alternative to separation of polar compounds could be ion-exchange chromatography, but this technique requires high concentrations of buffers in the mobile phase, which could have negative effects on the ionization source [25].…”

Section: Introductionmentioning

confidence: 99%

Application of the Hydrophilic Interaction Liquid Chromatography (HILIC-MS) Novel Protocol to Study the Metabolic Heterogeneity of Glioblastoma Cells

Šofranko,

Gondáš,

Murín

2024

Metabolites

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Glioblastoma is a highly malignant brain tumor consisting of a heterogeneous cellular population. The transformed metabolism of glioblastoma cells supports their growth and division on the background of their milieu. One might hypothesize that the transformed metabolism of a primary glioblastoma could be well adapted to limitations in the variety and number of substrates imported into the brain parenchyma and present it their microenvironment. Additionally, the phenotypic heterogeneity of cancer cells could promote the variations among their metabolic capabilities regarding the utilization of available substrates and release of metabolic intermediates. With the aim to identify the putative metabolic footprint of different types of glioblastoma cells, we exploited the possibility for separation of polar and ionic molecules present in culture media or cell lysates by hydrophilic interaction liquid chromatography (HILIC). The mass spectrometry (MS) was then used to identify and quantify the eluted compounds. The introduced method allows the detection and quantification of more than 150 polar and ionic metabolites in a single run, which may be present either in culture media or cell lysates and provide data for polaromic studies within metabolomics. The method was applied to analyze the culture media and cell lysates derived from two types of glioblastoma cells, T98G and U118. The analysis revealed that even both types of glioblastoma cells share several common metabolic aspects, and they also exhibit differences in their metabolic capability. This finding agrees with the hypothesis about metabolic heterogeneity of glioblastoma cells. Furthermore, the combination of both analytical methods, HILIC-MS, provides a valuable tool for metabolomic studies based on the simultaneous identification and quantification of a wide range of polar and ionic metabolites—polaromics.

show abstract

Section: Introductionmentioning

confidence: 99%

Application of the Hydrophilic Interaction Liquid Chromatography (HILIC-MS) Novel Protocol to Study the Metabolic Heterogeneity of Glioblastoma Cells

Šofranko,

Gondáš,

Murín

2024

Metabolites

View full text Add to dashboard Cite

show abstract

“…The majority of LC applications currently exploit reliable and reproducible separation of non-polar compounds through reversed-phase LC [22]. However, since a significant part of metabolites bear polar or ionic group, their separation by reversed-phase liquid chromatography often requires chemical modification through derivatization or relies on ion pairing [23,24]. Therefore, it seems to be demanding to introduce more simple methods for the separation of polar and ionic compounds by LC without prior derivatization, if possible.…”

Section: Introductionmentioning

confidence: 99%

Application of the HILIC-MS Novel Protocol to Study the Metabolic Heterogeneity of Glioblastoma Cells

Šofranko,

Gondáš,

Murín

2024

Preprint

View full text Add to dashboard Cite

Glioblastoma is a highly malignant brain tumor consisting of heterogeneous cellular population. The transformed metabolism of glioblastoma cells supports their growth and division on the background of their milieu. One might hypothesize that the transformed metabolism of a primary glioblastoma could be well adapted to limitations in the variety and number of substrates imported into the brain parenchyma and present it their microenvironment. Additionally, the phenotypic heterogeneity of cancer cells could promote the variations among their metabolic capabilities regarding the utilization of available substrates and release of metabolic intermediates. With the aim to identify the putative metabolic footprint of different types of glioblastoma cells, we exploited the possibility for separation of polar and ionic molecules present in culture media or cell lysates by hydrophilic interaction liquid chromatography (HILIC). The mass spectrometry (MS) was then used to identify and quantify the eluted compounds. The introduced method allows the detection and quantification of more than 150 polar and ionic metabolites in single run, those may be present either in culture media or cell lysates and provide data for polaromic studies within metabolomics. The method was applied to analyze the culture media and cell lysates derived from two types of glioblastoma cells, T98G and U118. The analysis revealed that even the both types of glioblastoma cells share several common metabolic aspects, they also exhibit differences in their metabolic capability. This finding agrees with the hypothesis about metabolic heterogeneity of glioblastoma cells. Furthermore, the combination of both analytical methods, HILIC-MS, provides a valuable tool for metabolomic studies based on the simultaneous identification and quantification of a wide range of polar and ionic metabolites - polaromics.

show abstract

Simultaneous Quantitation of Trimethylamine (Tma) and Trimethylamine-N-Oxide (Tmao) Using Liquid Chromatography with Tandem Mass Spectrometry

Valdivia-Garcia,

Bi,

Abaakil

et al. 2024

Preprint

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Other HPLC separations performed on hydrophobic stationary phases

Cited by 3 publications

References 54 publications

Application of the Hydrophilic Interaction Liquid Chromatography (HILIC-MS) Novel Protocol to Study the Metabolic Heterogeneity of Glioblastoma Cells

Application of the Hydrophilic Interaction Liquid Chromatography (HILIC-MS) Novel Protocol to Study the Metabolic Heterogeneity of Glioblastoma Cells

Application of the HILIC-MS Novel Protocol to Study the Metabolic Heterogeneity of Glioblastoma Cells

Simultaneous Quantitation of Trimethylamine (Tma) and Trimethylamine-N-Oxide (Tmao) Using Liquid Chromatography with Tandem Mass Spectrometry

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