OsZIP7 functions in xylem loading in roots and inter-vascular transfer in nodes to deliver Zn/Cd to grain in rice

Tan, Liwen; Zhu, Yan; Fan, Tony; Peng, Can; Wang, Jiurong; Sun, Liang; Chen, Caiyan

doi:10.1016/j.bbrc.2019.03.024

Cited by 157 publications

(104 citation statements)

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“…Zn is partially sequestered by OsHMA3 localized at the tonoplast in root cells (Cai et al, 2019) and the remaining Zn is translocated to the shoot by OsHMA2 localized at the pericycle cells (Yamaji et al, 2013). OsZIP7 was also implicated in Zn xylem loading although its exact role remains to be examined (Tan et al, 2019). At the node, Zn is preferentially delivered to developing organs such as new leaves and grains by OsZIP3 and OsHMA2.…”

Section: Discussionmentioning

confidence: 99%

“…Several ZIP members have been functionally characterized in terms of transport activity, expression patterns, and ectopic expression analysis. OsZIP1, OsZIP3, OsZIP4, OsZIP5, OsZIP7a, and OsZIP8 showed influx transport activity for Zn in yeast (Ramesh et al, 2003, Ishimaru et al, 2005Yang et al, 2009;Lee et al, 2010aLee et al, , 2010bTan et al, 2019). However, OsZIP2 in yeast and OsZIP6 in Xenopus oocytes did not show transport activity for Zn (Ramesh et al, 2003;Kavitha et al, 2015).…”

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confidence: 99%

“…On the other hand, overexpression of OsZIP4 and OsZIP5 causes decreased Zn accumulation in the shoots, but increased Zn accumulation in the roots (Ishimaru et al, 2007;Lee et al, 2010a). Based on these findings, OsZIP1 has been proposed to function in Zn uptake from soil (Ramesh et al, 2003, whereas OsZIP4, OsZIP5, OsZIP7, and OsZIP8 are involved in Zn translocation/distribution in the shoots (Ishimaru et al, 2005;Lee et al, 2010aLee et al, , 2010bSasaki et al, 2015;Tan et al, 2019). However, except for OsZIP3, the exact physiological roles of these ZIP genes in planta remain poorly understood.…”

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The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions

et al. 2020

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Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed that OsZIP9 was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore, OsZIP9 was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout of OsZIP9 significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A shortterm kinetic experiment with stable isotope 67 Zn showed that 67 Zn uptake in knockout lines was much lower than that in wildtype rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice.

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Section: Discussionmentioning

confidence: 99%

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The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions

et al. 2020

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“…The expression of OsZIP1 gene is seen only in root tissues under Zn starvation, but OsZIP3 is expressed in both roots and shoots under both Zn sufficient and deficient conditions (Ramesh et al, 2003). OsZIP7 and OsZIP8 are expressed in roots and shoots under Zn deficient condition (Yang et al, 2009;Tan et al, 2019). The OsZIP3 and OsZIP4 are highly expressed in the nodal regions under Zn deficiency condition (Sasaki et al, 2015).…”

Section: Ricementioning

confidence: 99%

Structure, Function, Regulation and Phylogenetic Relationship of ZIP Family Transporters of Plants

et al. 2020

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Zinc (Zn) is an essential micronutrient for plants and humans. Nearly 50% of the agriculture soils of world are Zn-deficient. The low availability of Zn reduces the yield and quality of the crops. The zinc-regulated, iron-regulated transporter-like proteins (ZIP) family and iron-regulated transporters (IRTs) are involved in cellular uptake of Zn, its intracellular trafficking and detoxification in plants. In addition to Zn, ZIP family transporters also transport other divalent metal cations (such as Cd 2+ , Fe 2+ , and Cu 2+). ZIP transporters play a crucial role in biofortification of grains with Zn. Only a very limited information is available on structural features and mechanism of Zn transport of plant ZIP family transporters. In this article, we present a detailed account on structure, function, regulations and phylogenetic relationships of plant ZIP transporters. We give an insight to structure of plant ZIPs through homology modeling and multiple sequence alignment with Bordetella bronchiseptica ZIP (BbZIP) protein whose crystal structure has been solved recently. We also provide details on ZIP transporter genes identified and characterized in rice and other plants till date. Functional characterization of plant ZIP transporters will help for the better crop yield and human health in future.

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“…To date, at least several transporters from the three transporter families, including the ZRT/IRT-like protein (ZIP) family, the heavy metal ATPase (HMA) family, and the metal tolerance protein (MTP) family, have been found to transport Zn in rice plants. OsZIP3, OsZIP7 and OsHMA2 were reported to be responsible for the preferential distribution of Zn to developing tissues (Satoh-Nagasawa et al, 2012;Takahashi et al, 2012;Yamaji et al, 2013;Sasaki et al, 2015;Tan et al, 2019). OsHMA3 and OsMTP1 were proposed to have a role in maintaining Zn homeostasis through sequestration of Zn into root and leaf vacuoles respectively (Ueno et al, 2010;Miyadate et al, 2011;Yuan et al, 2012;Menguer et al, 2013;Cai et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A high activity zinc transporter OsZIP9 mediates zinc uptake in rice

Yang

Liu

et al. 2020

The Plant Journal

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SUMMARY Zinc (Zn) is an essential micronutrient for most organisms including humans, and Zn deficiency is widespread in human populations, particularly in underdeveloped regions. Cereals such as rice (Oryza sativa) are the major dietary source of Zn for most people. However, the molecular mechanism underlying Zn uptake in rice is still not fully understood. Here, we report that a member of the ZIP (ZRT, IRT‐like protein) family, OsZIP9, contributes to Zn uptake in rice. It was expressed in the epidermal and exodermal cells of lateral roots, localized in the plasma membrane and induced during Zn deficiency. Yeast‐expressed OsZIP9 showed much higher Zn influx transport activity than other rice ZIP proteins in a wide range of Zn concentrations. OsZIP9 knockout rice plants showed a significant reduction in growth at low Zn concentrations, but could be rescued by a high Zn supply. Compared with the wild type, accumulation of Zn in root, shoot and grain was much lower in knockout lines, particularly with a low supply of Zn under both hydroponic and paddy soil conditions. OsZIP9 also showed Co uptake activity. Natural variation of OsZIP9 expression level is highly associated with Zn content in milled grain among rice varieties in the germplasm collection. Taken together, these results show that OsZIP9 is an important influx transporter responsible for the take up of Zn and Co from external media into root cells.

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OsZIP7 functions in xylem loading in roots and inter-vascular transfer in nodes to deliver Zn/Cd to grain in rice

Cited by 157 publications

References 30 publications

The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions

The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions

Structure, Function, Regulation and Phylogenetic Relationship of ZIP Family Transporters of Plants

A high activity zinc transporter OsZIP9 mediates zinc uptake in rice

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