OsWRKY45 alleles play different roles in abscisic acid signalling and salt stress tolerance but similar roles in drought and cold tolerance in rice

Zeng, Tao; Kou, Yanjun; Liu, Hongbo; Li, Xianghua; Xiao, Jinghua; Wang, Shiping

doi:10.1093/jxb/err144

Cited by 211 publications

(164 citation statements)

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“…The function of this cascade may be balanced via the negative feedback regulation of WRKY45-2 by WRKY13, and it also may be balanced by the autoregulation of the three WRKY proteins on their own genes. Because WRKY45-2 and WRKY13 are negatively involved in the regulation of rice response to abiotic stresses Tao et al, 2011), this cascade may provide rapid amplification of pathogen-induced defense signaling and balancing adaptation to different environmental stimuli by reprogramming the transcriptome.…”

Section: Resultsmentioning

confidence: 99%

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

et al. 2015

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Blast caused by fungal Magnaporthe oryzae is a devastating disease of rice (Oryza sativa) worldwide, and this fungus also infects barley (Hordeum vulgare). At least 11 rice WRKY transcription factors have been reported to regulate rice response to M. oryzae either positively or negatively. However, the relationships of these WRKYs in the rice defense signaling pathway against M. oryzae are unknown. Previous studies have revealed that rice WRKY13 (as a transcriptional repressor) and WRKY45-2 enhance resistance to M. oryzae. Here, we show that rice WRKY42, functioning as a transcriptional repressor, suppresses resistance to M. oryzae. WRKY42-RNA interference (RNAi) and WRKY42-overexpressing (oe) plants showed increased resistance and susceptibility to M. oryzae, accompanied by increased or reduced jasmonic acid (JA) content, respectively, compared with wild-type plants. JA pretreatment enhanced the resistance of WRKY42-oe plants to M. oryzae. WRKY13 directly suppressed WRKY42. WRKY45-2, functioning as a transcriptional activator, directly activated WRKY13. In addition, WRKY13 directly suppressed WRKY45-2 by feedback regulation. The WRKY13-RNAi WRKY45-2-oe and WRKY13-oe WRKY42-oe double transgenic lines showed increased susceptibility to M. oryzae compared with WRKY45-2-oe and WRKY13-oe plants, respectively. These results suggest that the three WRKYs form a sequential transcriptional regulatory cascade. WRKY42 may negatively regulate rice response to M. oryzae by suppressing JA signaling-related genes, and WRKY45-2 transcriptionally activates WRKY13, whose encoding protein in turn transcriptionally suppresses WRKY42 to regulate rice resistance to M. oryzae.

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Section: Resultsmentioning

confidence: 99%

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

et al. 2015

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“…4C). The WRKY45-1 transcription factor also negatively regulates SNAC1 expression (Tao et al, 2011). WRKY13 appeared to constitutively bind to WBOXa and WBOXc-C, but it had a stronger effect on WBOXa than WBOXc-C and it had a stronger effect during drought stress than during Xoo stress and no stress (Figs.…”

Section: Wrky13 Differentially Regulates Abiotic and Biotic Stress Rementioning

confidence: 97%

“…2; Qiu et al, 2007Qiu et al, , 2008. The opposite functions of WRKY13 appear to be at least partly performed by directly suppressing the expression of two transcription factor genes: WRKY45-1, which decreases both bacterial disease and abiotic stress resistance (Tao et al, 2009(Tao et al, , 2011, and SNAC1, which increases drought resistance (Hu et al, 2006). It does this by binding to site-and sequence-specific cis-elements on their promoters in vascular tissue or guard cells (Fig.…”

Section: Wrky13 Differentially Regulates Abiotic and Biotic Stress Rementioning

confidence: 99%

“…The WRKY45-2 transcription factor mediates broad-spectrum disease resistance but negatively regulates adaptation to salt, cold, and drought stresses in rice (Tao et al, 2009(Tao et al, , 2011. Interestingly, the allele of WRKY45-2, the WRKY45-1 that also encodes a transcription factor but with a difference of 10 amino acids from WRKY45-2, enhances rice resistance to M. oryzae but decreases resistance to the bacterial pathogens Xoo and Xanthomonas oryzae pv oryzicola and to cold and drought stresses (Tao et al, 2009(Tao et al, , 2011. Overexpression of the rice WRKY30 transcription factor increases rice resistance to fungal pathogen and drought stresses Shen et al, 2012).…”

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confidence: 99%

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Rice WRKY13 Regulates Cross Talk between Abiotic and Biotic Stress Signaling Pathways by Selective Binding to Different cis-Elements

et al. 2013

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Plants use a complex signal transduction network to regulate their adaptation to the ever-changing environment. Rice (Oryza sativa) WRKY13 plays a vital role in the cross talk between abiotic and biotic stress signaling pathways by suppressing abiotic stress resistance and activating disease resistance. However, it is not clear how WRKY13 directly regulates this cross talk. Here, we show that WRKY13 is a transcriptional repressor. During the rice responses to drought stress and bacterial infection, WRKY13 selectively bound to certain site-and sequence-specific cis-elements on the promoters of SNAC1 (for STRESS RESPONSIVE NO APICAL MERISTEM, ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR1/2, CUP-SHAPED COTYLEDON), the overexpression of which increases drought resistance, and WRKY45-1, the knockout of which increases both bacterial disease and drought resistance. WRKY13 also bound to two cis-elements of its native promoter to autoregulate the balance of its gene expression in different physiological activities. WRKY13 was induced in leaf vascular tissue, where bacteria proliferate, during infection, and in guard cells, where the transcriptional factor SNAC1 enhances drought resistance, during both bacterial infection and drought stress. These results suggest that WRKY13 regulates the antagonistic cross talk between drought and disease resistance pathways by directly suppressing SNAC1 and WRKY45-1 and autoregulating its own expression via site-and sequence-specific cis-elements on the promoters of these genes in vascular tissue where bacteria proliferate and guard cells where the transcriptional factor SNAC1 mediates drought resistance by promoting stomatal closure.

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“…Moreover, the OsWRKY30 protein also interacts with MAPKs suggesting its downstream interactive role with MAPK cascade . Another member of this family (OsWRKY45) also play an important role against drought stress is Arabidopsis and rice (Qiu and Yu, 2008;Tao et al, 2011). The Arabidopsis transcriptional factor (WRKY57) when transferred to rice confers drought tolerance.…”

Section: H2o2mentioning

confidence: 99%

Role of Reactive Oxygen Species and Contribution of New Players in Defense Mechanism under Drought Stress in Rice

Qureshi¹,

Munir²,

Rasul³

et al. 2018

IJAB

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Rice (Oryza sativa) falls among the staple food crops in different parts of the globe. In current scenario of climate change, drought stress leads to significant decrease in crop production. It has negative effect on rice growth and development by affecting cellular, physiological and molecular processes. Photo-respiration increases under drought stress leads to overproduction of reactive oxygen species (ROS) in different organelles of the cell like chloroplasts, mitochondria and peroxisomes etc., which induce severe oxidative stress in rice. Over production of ROS can cause damage to proteins, lipids and DNA leading to lipid peroxidation, proteins oxidation, mutation, DNA damage that can lead to cell death. Under drought stress, ROS turn over in various organelles overload antioxidant quenching mechanism leading to oxidative damage. Oxidative stress can be overcome by the scavenging system, which consists of enzymatic and non-enzymatic antioxidants. Moreover, ROS also acts as signaling molecule and triggers defense mechanism through specific signal transduction network under stress. Under stress condition, activation of molecular cascades is initiated through the perception of stress that leads to the activation of signal transduction pathway including expression of transcription factors and stress related genes. Understanding of this regulatory mechanism of plant development and growth in drought-ROS stress can be promising in the development of improved transgenic rice under this stress. This review will provide an overview of ROS synthesis and signaling pathway under drought condition in rice.

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OsWRKY45 alleles play different roles in abscisic acid signalling and salt stress tolerance but similar roles in drought and cold tolerance in rice

Cited by 211 publications

References 53 publications

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

The WRKY45-2 WRKY13 WRKY42 Transcriptional Regulatory Cascade Is Required for Rice Resistance to Fungal Pathogen

Rice WRKY13 Regulates Cross Talk between Abiotic and Biotic Stress Signaling Pathways by Selective Binding to Different cis-Elements

Role of Reactive Oxygen Species and Contribution of New Players in Defense Mechanism under Drought Stress in Rice

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