OsVIN2 encodes a vacuolar acid invertase that affects grain size by altering sugar metabolism in rice

Xu, Xiaoyong; Ren, Yulong; Wang, Chunming; Zhang, Huan; Wang, Fan; Chen, Jun; Liu, Xi; Zheng, Tianhui; Cai, Maohong; Zeng, Zhaoqiong; Zhou, Liang; Zhu, Shanshan; Tang, Weijie; Wang, Jiulin; Guo, Xiuping; Jiang, Ling; Chen, Saihua; Wan, Jianmin

doi:10.1007/s00299-019-02443-9

Cited by 28 publications

(34 citation statements)

References 64 publications

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“…The INV3-YFP fusion protein localized to the nucleus ( Figure 7B), an observation consistent with a previous report [41]. OsVIN2 has been reported to be a dual localization protein, and it is not detectable in the vacuole because the fluorescence signal is easily degraded in the acidic vacuolar lumen in the light and may be transported to the vacuole through PVCs [41,50,51]. Thus, the expression pattern of OsINV3 is consistent with the role of OsINV3 in influencing grain and panicle size.…”

Section: Osinv3 Expression Pattern and Protein Subcellular Localizationsupporting

confidence: 93%

“…A plasmid containing the yellow fluorescent protein (YFP) gene tagged to OsINV3 wild-type cDNA driven by a native promoter (INV3-YFP) was transiently expressed in rice protoplast. The INV3-YFP fusion protein localized to the nucleus ( Figure 7B), an observation consistent with a previous report [41]. OsVIN2 has been reported to be a dual localization protein, and it is not detectable in the vacuole because the fluorescence signal is easily degraded in the acidic vacuolar lumen in the light and may be transported to the vacuole through PVCs [41,50,51].…”

Section: Osinv3 Expression Pattern and Protein Subcellular Localizationsupporting

confidence: 91%

“…Moreover, many studies have showed that VINs play key roles in plant growth by modulating cell expansion [33,53,54]. At the cellular level, the cell size of sg2-1 and sg2-2 mutants was affected by cell expansion in spikelet hulls (Figure 2), which is consistent with a previous report [41]. Map-based cloning and Mutmap analysis demonstrated that a SNP in the exon of OsINV3 resulted in the mutant phenotype (Figure 3).…”

Section: Sg2 Is a Novel Mutant Allele Of Osinv3supporting

confidence: 88%

“…In rice, several factors that control grain size have been identified, but the mechanisms remain largely unknown. In this study, we identified SG2, which encodes a vacuolar invertase, and is a novel allele of OsINV3, also known as OsVIN2 [40,41]. The sg2-1 and sg2-2 mutants showed reduced grain length, grain width, and 1000-grain weight (Figure 1).…”

Section: Sg2 Is a Novel Mutant Allele Of Osinv3mentioning

confidence: 96%

“…These results suggested that LOC_Os02g01590 represents the SG2 gene. This gene encodes a vacuolar invertase, OsINV3, which is involved in sink strength determination, mainly by regulation of grain size, assimilates partitioning to grain and affects grain size by altering sugar metabolism [40,41]. To identify the gene responsible for the sg2 phenotype, we obtained F1 and F2 progenies from the crosses between mutants and WT.…”

Section: Genetic Analysis and Map-based Cloning Of The Sg2-1 And Sg2-mentioning

confidence: 99%

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OsINV3 and Its Homolog, OsINV2, Control Grain Size in Rice

Deng

Han

et al. 2020

IJMS

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Vacuolar invertase is involved in sugar metabolism and plays a crucial role in plant growth and development, thus regulating seed size. However, information linking vacuolar invertase and seed size in rice is limited. Here we characterized a small grain mutant sg2 (grain size on chromosome 2) that showed a reduced in grain size and 1000-grain weight compared to the wild type. Map-based cloning and genetic complementation showed that OsINV3 is responsible for the observed phenotype. Loss-of-function of OsINV3 resulted in grains of smaller size when compared to the wild type, while overexpression showed increased grain size. We also obtained a T-DNA insertion mutant of OsINV2, which is a homolog of OsINV3 and generated double knockout (KO) mutants of OsINV2 and OsINV3 using CRISPR/Cas9. Genetic data showed that OsINV2, that has no effect on grain size by itself, reduces grain length and width in the absence of OsINV3. Altered sugar content with increased sucrose and decreased hexose levels, as well as changes vacuolar invertase activities and starch constitution in INV3KO, INV2KO, INV3KOINV2KO mutants indicate that OsINV2 and OsINV3 affect sucrose metabolism in sink organs. In summary, we identified OsINV3 as a positive regulator of grain size in rice, and while OsINV2 has no function on grain size by itself. In the absence of OsINV3, it is possible to detect a role of OsINV2 in the regulation of grain size. Both OsINV3 and OsINV2 are involved in sucrose metabolism, and thus regulate grain size. Our findings increase our understanding of the role of OsINV3 and its homolog, OsINV2, in grain size development and also suggest a potential strategy to improve grain yield in rice.

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Section: Osinv3 Expression Pattern and Protein Subcellular Localizationsupporting

confidence: 93%

Section: Osinv3 Expression Pattern and Protein Subcellular Localizationsupporting

confidence: 91%

Section: Sg2 Is a Novel Mutant Allele Of Osinv3supporting

confidence: 88%

Section: Sg2 Is a Novel Mutant Allele Of Osinv3mentioning

confidence: 96%

Section: Genetic Analysis and Map-based Cloning Of The Sg2-1 And Sg2-mentioning

confidence: 99%

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OsINV3 and Its Homolog, OsINV2, Control Grain Size in Rice

Deng

Han

et al. 2020

IJMS

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Improvement of yield and grain quality by periodic cold plasma treatment with rice plants in a paddy field

Hashizume

Kitano

Mizuno

et al. 2020

Plasma Processes & Polymers

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The application of cold plasma has attracted considerable attention in the field of agriculture. We examined the effects of plasma irradiation and treatment with plasma‐activated Ringer's lactate solution (PAL) on rice seedlings in a paddy field. Irradiation during the vegetative growth period increased seedling growth, panicle number, and grain yield. In contrast, treatment during the reproductive growth period had a negative or no effect. Moreover, treatment with PAL solution promoted the growth of the main stem, such as grain number and panicle weight, and grain quality, but the grain yield from the whole plant was decreased. The results suggest that cold plasma treatment of rice seedlings is effective for improving plant growth, grain yield, and grain quality.

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