Osthole inhibits the invasive ability of human lung adenocarcinoma cells via suppression of NF-κB-mediated matrix metalloproteinase-9 expression

Kao, Shang Jyh; Su, Jen Liang; Chen, Chi Kuan; Yu, Ming Chih; Bai, Kuan Jen; Chang, Jer‐Hwa; Bien, Mauo Ying; Yang, Shun Fa; Chien, Ming Hsien

doi:10.1016/j.taap.2012.03.020

Cited by 62 publications

(53 citation statements)

References 44 publications

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“…Amounts of studies showed that Osthole regulates cell apoptosis, proliferation and invasion via multiple signaling pathways in many cancer cells [13,15,16]. In addition, it has been reported in recent studies that Osthole has essential roles in brain functions by protecting neurons [17,18,19,20,21], suggesting that Osthole could penetrate the blood-brain barrier for the chemotherapy drugs of brain tumours.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have revealed that Osthole may have anti-inflammatory [6], vasorelaxant [7], and antiallergic [8] effects, as well as the prophylactic effects in hepatitis [9]. In addition, Osthole, as widely reported, exhibits an anti-cancer effect by suppressing cancer cell growth and enhancing apoptosis [10,11,12,13,14]. Given its significant activities in pharmacology as described above, Osthole is becoming a very promising lead compound for drug discovery.…”

Section: Introductionmentioning

confidence: 99%

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Osthole Exhibits Anti-Cancer Property in Rat Glioma Cells Through Inhibiting PI3K/Akt and MAPK Signaling Pathways

Ding

Wei

Song

et al. 2013

Cell Physiol Biochem

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Aims: The purpose of this study was to investigate how Osthole affects glioma cell proliferation, apoptosis, invasion and migration. Methods: Rat glioma cells were treated with different concentrations of Osthole (0 µM, 25 µM, 50 µM, and 100 µM). Cell proliferation was assessed by measuring PCNA expression and CCK8 assay at different time points. Apoptosis was evaluated by measuring the expression of pro-apoptotic protein including Bax, Bcl2, PARP, and cleaved Caspase3, and of anti-apoptotic protein Survivin. Cell migration and invasion were assessed using different methods. Signaling pathways such as PI3K/Akt and MAPK, which are involved in the development of glioma cells, were also investigated in this study. Results: Treatment with Osthole markedly inhibits glioma cell proliferation, as assessed by western blot with the PCNA antibody. Osthole also induces cell apoptosis by upregulating the expression of pro-apoptotic proteins, and by reducing the expression of anti-apoptotic factors. Moreover, C6 cell migration and invasion were efficiently inhibited in groups treated with Osthole, compared to the control group. Additionally, inhibition of PI3K/Akt and MAPK signaling pathway was also observed in C6 cells treated with Osthole. Conclusions: Our findings showed an anti-cancer effect of Osthole on glioma cells, including the proliferation inhibition, apoptosis induction, and migration/invasion inhibition. Further investigation in C6 glioma cells implicated the role of Osthole in essential pathways controlling glioma cell progression. Taken together, our data suggested that Osthole may have a potential application in glioma therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Osthole Exhibits Anti-Cancer Property in Rat Glioma Cells Through Inhibiting PI3K/Akt and MAPK Signaling Pathways

Ding

Wei

Song

et al. 2013

Cell Physiol Biochem

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“…Osthole ( Figure 1) is a major bioactive ingredient isolated from Cnidium monnieri (L.) cusson [1,2], which has been used for treatment of pain in female genitalia, impotence, and suppurative dermatitis (as an antipruritogenic agent). Osthole exhibits various pharmacological activities, including in vitro/in vivo antitumor effects [3][4][5][6][7][8], alleviation of hyperglycemia and hypolipidemia [9,10], and has been proposed the possibility of its development as a promising lead compound for drug discovery [11,12]. However, the clinical utility of this phytochemical is limited due to its low bioavailability in vivo [13][14][15][16], and some structural modifications are required for sufficient bioavailability upon oral administration.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics Analysis of Copen, a Novel Antitumor Semi Synthetic Derivative of Osthole, in Rats after Intragastric and Intravenous Administration

Yang¹,

Zhou²,

Hu³

et al. 2017

J Bioequiv Availab

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“…Moreover, accumulating evidence has shown that osthole exerts anti-cancer effects through inducing cell cycle arrest and apoptotic cell death [16,17,18]. Recent studies have also shown that osthole is able to inhibit the migration and invasion of cancer cells through suppression of MMP-2 and MMP-9 enzyme activities [19,20]. However, the exact molecular mechanism of osthole induced anticancer effect is not completely understood, and there are no results to date of the effect of osthole on cisplatin-related chemotherapeutic resistance in human lung cancer cells.…”

Section: Introductionmentioning

confidence: 99%

NBM-T-BMX-OS01, an Osthole Derivative, Sensitizes Human Lung Cancer A549 Cells to Cisplatin through AMPK-Dependent Inhibition of ERK and Akt Pathway

Chen

Zhou

Ning

et al. 2015

Cell Physiol Biochem

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Background: Drug combination therapies using cisplatin and natural products are common practice in the treatment of human lung cancer. Osthole is a natural compound extracted from a number of medicinal plants and has been shown to exert strong anticancer activities with low toxicity. Methods: In the present study, NBM-T-BMX-OS01 (BMX), derived from the semi-synthesis of osthole, was evaluated in cisplatin treated A549 cells to investigate its effect on cisplatin resistance in human lung cancer. The anticancer effect of BMX were measured by cell viablity‚ colony formation‚ TUNEL staining‚ flow cytometry and cell cycle assay. The fluorescence staining was performed to detect intracellular and mitochondrial reactive oxygen species (ROS) generation. Western blot analysis, antagonists pretreatment and small interfering RNA (siRNA) transfection were used to determine the potential mechanism. Results: It was found that, in comparison with single cisplatin treatment, the combination of BMX and cisplatin resulted in greater efficacy in inhibition of proliferation and colony formation, apoptosis induction and cell cycle arrest. The results of fluorescence staining showed that the combination effect of BMX and cisplatin was due to oxidative stress induced by mitochondrial ROS generation. In addition, BMX significantly attenuated the phosphorylation of ERK and Akt, two important pro-survival kinases. In contrast, BMX inhibited the activation of AMPK, and knockdown of AMPK using specific siRNA partially reversed BMX-induced inhibition of ERK and Akt, as well as its synthetic effects on cisplatin induced anticancer activity in A549 cells. Conclusion: Taken together, this study provides that BMX might modulate cisplatin resistance through AMPK-ERK and AMPK-Akt pathways. These results also support the role of BMX as a potential drug candidate for use in combination with cisplatin in the treatment of human lung cancer.

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Osthole inhibits the invasive ability of human lung adenocarcinoma cells via suppression of NF-κB-mediated matrix metalloproteinase-9 expression

Cited by 62 publications

References 44 publications

Osthole Exhibits Anti-Cancer Property in Rat Glioma Cells Through Inhibiting PI3K/Akt and MAPK Signaling Pathways

Osthole Exhibits Anti-Cancer Property in Rat Glioma Cells Through Inhibiting PI3K/Akt and MAPK Signaling Pathways

Pharmacokinetics Analysis of Copen, a Novel Antitumor Semi Synthetic Derivative of Osthole, in Rats after Intragastric and Intravenous Administration

NBM-T-BMX-OS01, an Osthole Derivative, Sensitizes Human Lung Cancer A549 Cells to Cisplatin through AMPK-Dependent Inhibition of ERK and Akt Pathway

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