Osthole Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Human Cervical Carcinoma HeLa Cells

Abstract

Purpose. To study the effect of osthole extract on proliferation, migration, invasion, and apoptosis of human cervical carcinoma HeLa cells and investigate its underlying mechanism. Methods. HeLa cells were exposed to osthole at various concentrations. Cell viability, migration, and invasion were detected by MTT assay, scratch wound-healing assay, and invasion assay, respectively. The proportion of cells undergoing apoptosis was analyzed by flow cytometry. Western blot and RT-qPCR were performed to determine changes in the expression of key factors in the Wnt/β-catenin signaling pathway. Results. The osthole extract effectively inhibited the proliferation, migration, and invasion potential of HeLa cells in a dose-dependent manner. The rate of apoptosis induction in HeLa cells treated with the osthole extract for 48 h was significantly higher than that of the untreated controls. Outcomes of the western blotting analysis and RT-qPCR showed that the expression of β-catenin, c-Myc, cyclin D1, survivin, and MMP-9 was significantly inhibited. Conclusion. Osthole could significantly inhibit the malignant behavior of HeLa cells and induce cellular apoptosis. Inactivation of the Wnt/β-catenin signaling pathway by osthole may be a mechanism to control cancer metastasis.

Potential mechanisms of osthole against bladder cancer cells based on network pharmacology, molecular docking, and experimental validation

“…Xu et al revealed that osthole suppresses gastric cancer cell proliferation [ 36 ]. Yin et al demonstrated that osthole could inhibit proliferation, invasion, and migration of human cervical cancer [ 37 ]. Sun et al revealed that osthole induces apoptosis in human tongue cancer [ 38 ].…”

“…Liang et al determined that osthole was able suppress the proliferation of endometrial cancer cells by inhibiting the PI3K-AKT pathway [ 11 ]. Yin et al discovered that osthole could block the Wnt/β-catenin pathway, influencing the proliferation and invasion of human cervical carcinoma [ 37 ]. Dai et al concluded that osthole could inhibit triple-negative breast cancer by suppressing Stat3 [ 49 ].…”

Potential Mechanisms of Osthole against Bladder Cancer Based on Network Pharmacology, Molecular Docking, and Experimental Validation

“…Xu et al revealed that osthole suppresses gastric cancer cell proliferation [26]. Yin et al demonstrated that osthole could inhibit proliferation, invasion, and migration of human cervical cancer [27]. Sun et al revealed that osthole induces apoptosis in human tongue cancer [28].…”

“…Liang et al determined that osthole was able suppress the proliferation of endometrial cancer cells by inhibiting the PI3K-AKT pathway [9]. Yin et al discovered that osthole could block the Wnt/β-catenin pathway, in uencing the proliferation and invasion of human cervical carcinoma [27]. Dai et al concluded that osthole could inhibit triple-negative breast cancer by suppressing Stat3 [37].…”

Osthole: An up-to-date review of its anticancer potential and mechanisms of action

“…IC 50 values of Osthole on HeLa and Me-180 were 45.01 ± 3.91 μM and 88.95 ± 0.13 μM, respectively, while values were 8.35 ± 1.62 μM and 10.29 ± 0.18 μM for LRRK, respectively ( Pan et al, 2021 ). Osthole (50, 100, 200 μg/ml) extract also effectively promoted apoptosis and inhibited cellular proliferation, migration, and invasion potential in a cervical cancer cell line (HeLa) in a dose-dependent manner through inactivation of the Wnt/ β -catenin pathway ( Yin et al, 2021 ).…”