Osterix is regulated by Erk1/2 during osteoblast differentiation

Choi, You Hee; Gu, Young-Mi; Oh, Jae-Wook; Lee, Kwang-Youl

doi:10.1016/j.bbrc.2011.10.097

Cited by 43 publications

(33 citation statements)

References 25 publications

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“…Thus, it is likely that osteogenic genes containing adjacent Runx2 and Osx sites are regulated in a similar fashion. These results are consistent with data reported by Lee and co-workers (34) showing cooperation of Osx and Runx2 in the regulation of osteogenic marker genes during differentiation of adipose stem cells into osteoblasts.…”

Section: Discussionsupporting

confidence: 93%

“…MAPKs have been shown to phosphorylate and increase the transcriptional activities of Runx2 and Osx (6,(32)(33)(34)42) Moreover, the MAPK phosphorylation sites in Osx and Runx2 identified so far are localized within the regions described above as being involved in their physical interaction (33,42,43). Therefore, we tested the effect of the phosphorylation state of Osx and Runx2 on their transcriptional cooperation.…”

Section: P38 and Erk Mapk Activities Are Necessary For Functional Andmentioning

confidence: 99%

“…Induction of Osx expression requires the activation of Dlx5 through p38-mediated phosphorylation (16). Furthermore, it has been shown that Osx itself is a substrate for p38 (33) and ERK MAPK (34,35), which increases recruitment of transcriptional coactivators (33). Similarly, Runx2 is strongly regulated through direct p38 and ERK MAPK phosphorylation.…”

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confidence: 99%

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Mitogen-activated Protein Kinase (MAPK)-regulated Interactions between Osterix and Runx2 Are Critical for the Transcriptional Osteogenic Program

Artigas

Ureña

Rodríguez-Carballo

et al. 2014

Journal of Biological Chemistry

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Background: Osterix and Runx2 are master genes that transcriptionally promote osteoblast differentiation. Results: Osterix and Runx2 cooperate to induce osteogenic genes by binding to promoters and interacting with each other. Conclusion: Osterix and Runx2 exhibit cooperation, subject to further regulation by MAPK signals, during osteogenesis. Significance: A network of interactions between transcription factors provides a circuit that drives the osteoblast differentiation program.

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Section: Discussionsupporting

confidence: 93%

Section: P38 and Erk Mapk Activities Are Necessary For Functional Andmentioning

confidence: 99%

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Mitogen-activated Protein Kinase (MAPK)-regulated Interactions between Osterix and Runx2 Are Critical for the Transcriptional Osteogenic Program

Artigas

Ureña

Rodríguez-Carballo

et al. 2014

Journal of Biological Chemistry

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“…124 Controversial results regarding the role of the PI3K/Akt pathway in osteogenesis have also been presented. [125][126][127][128][129][130][131] Studies have shown that imatinib stimulates osteogenesis by inhibiting PDGFR/PI3K/Akt signaling in MSCs. [132][133][134] The reason for this effect is not well understood.…”

mentioning

confidence: 99%

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Chen

Crawford

Chen

et al. 2013

Tissue Engineering Part B: Reviews

197

156

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Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types and have been widely used in tissue engineering application. In tissue engineering, a scaffold, MSCs and growth factors are used as essential components and their interactions have been regarded to be important for regeneration of tissues. A critical problem for MSCs in tissue engineering is their low survival ability and functionality. Most MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity including application of growth factors, overexpression of stem cell regulatory genes, and improvement of biomaterials for scaffolds. The effects of these approaches on MSCs have been associated with activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The pathway plays central regulatory roles in MSC survival, proliferation, migration, angiogenesis, cytokine production, and differentiation. In this review, we summarize and discuss the literatures related to the roles of the PI3K/Akt pathway in the functionalities of MSCs and the involvement of the pathway in biomaterials-increased MSC functionalities. Biomaterials have been modified in their properties and surface structure and loaded with growth factors to increase MSC functionalities. Several studies demonstrated that the biomaterials-increased MSC functionalities are mediated by the activation of the PI3K/Akt pathway. MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity including application of growth factors, overexpression of stem cell regulatory genes, and improvement of biomaterials for scaffolds. The effects of these approaches on MSCs have been associated with activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The pathway plays central regulatory roles in MSC survival, proliferation, migration, angiogenesis, cytokine production, and differentiation. In this review, we summarize and discuss the literatures related to the roles of the PI3K/Akt pathway in the functionalities of MSCs and the involvement of the pathway in biomaterials-increased MSC functionalities. Biomaterials have been modified in their properties and surface structure and loaded with growth factors to increase MSC functionalities. Several studies demonstrated that the biomaterials-increased MSC functionalities are mediated by the activation of the PI3K/Akt pathway.

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“…Osterix is also up-regulated by BMP2 during osteoblastic differentiation (Matsubara et al, 2008;Nakashima et al , 2002). Osterix is thought to function downstream of RUNX2 during osteoblast differentiation (Choi et al , 2011;Matsubara et al , 2008). Recently, Matsubara et al (2008) obtained from the other 2 cell types.…”

Section: Resultsmentioning

confidence: 99%

Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell

Park¹,

Goo²,

Choi³

et al. 2013

International Journal of Industrial Entomology

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Bone morphogenetic proteins (BMPs) belong to the transforming growth factor (TGF-b) superfamily and are involved in osteoblastic differentiation. The largest TGF-b superfamily subgroup shares genetic homology with human BMPs (hBMPs) and silkworm decapentaplegic (dpp). In addition, hBMPs are functionally interchangeable with Drosophila dpp . Bombyx mori dpp may induce bone formation in mammalian cells. To test this hypothesis, we synthesized the 1,285-base pairs cDNA of full-length B. mori dpp using total RNAs obtained from the fat body of 3-day-old of the 5 th instar larvae and cloned the cDNA into the pCEP4 mammalian expression vector. Next, B. mori dpp was expressed in C3H10T1/2 cells. The target cells transfected with the pCEP4-Bm dpp plasmid showed biological functions similar to those of osteogenic differentiation induction growth factors such as hBMPs. We determined the relative mRNA expression rates of Runt-related transcription factor 2 (RUNX2), osterix, osteocalcin, and alkaline phosphatase (ALP) to validate the osteoblast-specific differentiation effects of B. mori dpp by performing quantitative real-time RT-PCR. Interestingly, mRNA expression levels of the 3 marker genes except RUNX2, in cells expressing B. mori dpp were much higher than those in control cells and C3H10T1/2 cells transfected with pCEP4. These results suggested that B. mori dpp signaling regulates osterix expression during osteogenic differentiation via RUNX2-independent mechanisms.

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Osterix is regulated by Erk1/2 during osteoblast differentiation

Cited by 43 publications

References 25 publications

Mitogen-activated Protein Kinase (MAPK)-regulated Interactions between Osterix and Runx2 Are Critical for the Transcriptional Osteogenic Program

Mitogen-activated Protein Kinase (MAPK)-regulated Interactions between Osterix and Runx2 Are Critical for the Transcriptional Osteogenic Program

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Functional analysis of Bombyx mori Decapentaplegic gene for bone differentiation in a mammalian cell

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