Osterix Enhances BMSC-associated Osseointegration of                Implants

Xu, Binbin; Zhang, J.; Brewer, Erika; Tu, Qisheng; Yu, Liming; Tang, Jintian; Krebsbach, Paul H.; Wieland, M; Chen, Jake Y.

doi:10.1177/0022034509346928

Cited by 54 publications

(55 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The titanium implants (1.05 mm in diameter and 2 mm in length, Institute Straumann AG, Basel, Switzerland) were SLA (sand-blasting and acid etching) surfaced and the surgery was performed as previously described (Xu et al, 2009). Briefly, two implantation sites, one in each femur, were prepared on the anterior-distal surface of the www.ecmjournal.org SG Yan et al Implant osseointegration enhanced by SATB2 femora in 20 10-week-old B6D2F1 mice (Jax #100006, Jackson Laboratory, Bar Harbor, ME, USA).…”

Section: Animal Surgery and Bmsc Applicationmentioning

confidence: 99%

“…The percentage of new bone edges in direct contact with the implant surface was also determined (Xu et al, 2009).…”

Section: Histomorphometric Analysismentioning

confidence: 99%

“…According to the histomorphometric measurements on the H&E stained sections, the newly formed bone was restricted to a 0.5 mm area surrounding the implant. Thus, at a 3D level the Hounsfield Unit (HU) of this newly formed bone area was determined using eFilm Workstation 2.12 (Merge Technologies Inc., Milwaukee, USA) as we reported previously (Xu et al, 2009). …”

Section: Micro-computed Tomography (μCt) Analysismentioning

confidence: 99%

“…The scarceness of bone forming cells in the implant site also poses the difficulty in early implant placement and immediate loading and thus limits the use of titanium implants in certain patients. Our previous study demonstrated that transplanted BMSCs can be recruited from circulation to the implantation sites and participate in implant osseointegration (Xu et al, 2009). Based on this study, BMSCs were labelled with GFP prior to transplantation into the implant sites and GFP staining was performed to determine the BMSC fate.…”

Section: Sg Yan Et Al Implant Osseointegration Enhanced By Satb2mentioning

confidence: 99%

“…Osteoblast progenitors are recruited from bone marrow and are involved in bone regeneration from peripheral circulation (Li et al, 2008). Transplanted BMSCs can be recruited from peripheral circulation to implantation sites and participate in the osseointegration of the titanium implants (Xu et al, 2009). However, there is only a small population of mesenchymal stem cells with osteogenic potential in the implant region, which explains the slow bone regeneration around implants after surgery.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Transcription factor and bone marrow stromal cells in osseointegration of dental implants

Yan

Zhang

et al. 2013

eCM

View full text Add to dashboard Cite

Titanium implants are widely used in dental clinics and orthopaedic surgery. However, bone formation surrounding the implant is relatively slow after inserting the implant. The current study assessed the effects of bone marrow stromal cells (BMSCs) with forced expression of special AT-rich sequence-binding protein 2 (SATB2) on the osseointegration of titanium implants. To determine whether SATB2 overexpression in BMSCs can enhance the osseointegration of implants, BMSCs were infected with the retrovirus encoding Satb2 (pBABE-Satb2) and were locally applied to bone defects before implanting the titanium implants in the mouse femur. Seven and twentyone days after implantation, the femora were isolated for immunohistochemical (IHC) staining, haematoxylin eosin (H&E) staining, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), and micro-computed tomography (μCT) analysis. IHC staining analysis revealed that SATB2-overexpressing BMSCs were intensely distributed in the bone tissue surrounding the implant. Histological analysis showed that SATB2-overexpressing BMSCs significantly enhanced new bone formation and bone-to-implant contact 3 weeks after implantation. Real-time qRT-PCR results showed that the local delivery of SATB2-overexpressing BMSCs enhanced expression levels of potent osteogenic transcription factors and bone matrix proteins in the implantation sites. μCT analysis demonstrated that SATB2-overexpressing BMSCs significantly increased the density of the newly formed bone surrounding the implant 3 weeks postoperatively. These results conclude that local delivery of SATB2-overexpressing BMSCs significantly accelerates osseointegration of titanium implants. These results provide support for future pharmacological and clinical applications of SATB2, which accelerates bone regeneration around titanium implants.

show abstract

Section: Animal Surgery and Bmsc Applicationmentioning

confidence: 99%

“…The percentage of new bone edges in direct contact with the implant surface was also determined (Xu et al, 2009).…”

Section: Histomorphometric Analysismentioning

confidence: 99%

Section: Micro-computed Tomography (μCt) Analysismentioning

confidence: 99%

Section: Sg Yan Et Al Implant Osseointegration Enhanced By Satb2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcription factor and bone marrow stromal cells in osseointegration of dental implants

Yan

Zhang

et al. 2013

eCM

View full text Add to dashboard Cite

show abstract

Adherence to gastroprotection during cyclooxygenase 2 inhibitor treatment and the risk of upper gastrointestinal tract events: A population‐based study

Valkhoff¹,

Soest²,

Mazzaglia

et al. 2012

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. Guidelines recommend coprescription of gastroprotective agents (GPAs) in patients receiving cyclooxygenase 2 inhibitors (coxibs) who are at high risk of upper gastrointestinal (UGI) tract complications (i.e., patients with a previous complicated ulcer or with multiple risk factors). Suboptimal GPA adherence has been shown to diminish the gastroprotective effect during use of nonselective nonsteroidal antiinflammatory drugs, but little is known about the effect of GPA adherence during coxib treatment. We undertook this study to determine the association between GPA adherence and UGI tract events among patients receiving coxibs.Methods. Using primary care data from 3 databases, we conducted a case-control study in a cohort of patients age >50 years who were newly starting treatment with coxibs and concomitantly taking GPAs. Patients who had a UGI tract event (bleeding or symptomatic ulcer) were matched to event-free controls for age, sex, database, and calendar date. Coxib treatment intervals were defined as consecutive coxib prescriptions with intervening gaps not exceeding the duration of the previous coxib prescription. Adherence to GPAs was calculated as the proportion of days of coxib treatment covered by a GPA prescription. Odds ratios (ORs) with 95% confidence intervals (95% CIs) were calculated using conditional logistic regression analysis.Results. The coxib plus GPA-treated cohort consisted of 14,416 coxib-treated patients who received GPAs for at least 1 day, yielding 16,442 coxib treatment intervals in which a GPA was coprescribed. Most patients were treated with coxibs for <30 days. Seventyfour patients had a UGI tract event during or shortly after a coxib treatment interval in which a GPA was coprescribed, with an incidence rate of 11.9 (95% CI Study results were presented in part by Drs. Valkhoff and

show abstract

BESSICC, a COSMO‐RS based tool for in silico solvent screening of biocatalyzed reactions

Braiuca

Khaliullin

Švedas

et al. 2012

Biotech & Bioengineering

View full text Add to dashboard Cite

Many enzymatic reactions are near-equilibrium reactions. This often limits final yield and hence application of biocatalyzed processes in the industrial production. The most widely applied strategy to overcome this issue is solvent selection. It must be underlined that measuring the equilibrium position experimentally is a difficult and time-consuming procedure and any tool for predicting the solvent effect on the reaction equilibrium can be very valuable. The present work reports on the development of BESSICC, an algorithm to calculate the effect of medium composition on biocatalyzed reactions equilibrium. It is based on COSMO-RS calculation of activity coefficients of all the species in the reaction mixture and minimization of Gibbs free energy of the reaction. Starting from one single experimental measurement of the equilibrium position for a given biocatalyzed reaction it can predict the yield of the reaction in any other solvent or solvent mixture. Predictions are accurate, the errors of prediction are in average below 25% for the esterification of dodecanoic acid with menthol and below 65% for esterification of 1-dodecanoic acid with 1-dodecanol. The best predictions show an error well below 5%.

show abstract

Osterix Enhances BMSC-associated Osseointegration of Implants

Cited by 54 publications

References 20 publications

Transcription factor and bone marrow stromal cells in osseointegration of dental implants

Transcription factor and bone marrow stromal cells in osseointegration of dental implants

Adherence to gastroprotection during cyclooxygenase 2 inhibitor treatment and the risk of upper gastrointestinal tract events: A population‐based study

BESSICC, a COSMO‐RS based tool for in silico solvent screening of biocatalyzed reactions

Contact Info

Product

Resources

About