Osteosarcoma Cells Differentiate into Phenotypes from all Three Dermal Layers

Russinoff, Scott; Miran, Sara; Gowda, Ashok; Lucas, Paul A.

doi:10.1007/s11999-011-1946-3

Cited by 3 publications

(4 citation statements)

References 52 publications

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“…Moreover, disrupted apoptosis play a crucial role in drugresistance of tumor cells, and it has become a significant obstacle for the successful management of patients with chondrosarcoma (22). Since current chemotherapy regimens have limited success in improving metastasis-free survival and limited by the severe toxicity of conventional agents, the therapeutic bottleneck of chondrosarcoma still remains unconquered (23,24). Natural products are important to discover new drugs.…”

Section: Discussionmentioning

confidence: 99%

Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells

Liang

et al. 2013

International Journal of Oncology

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Gallic acid (GA), a natural agent, is widely distri-buted in plants with a range of biological effects and has been of potential interest as anticancer agent. However, its effects on chondrosarcoma cell apoptosis are still undefined. In the present study, the possible mechanisms of GA-induced apoptosis were explored in SW1353 cells, a human chondrosarcoma cell line. Our results showed that GA inhibited cell viability dose- and time-dependently. Morphological examination of GA-treated cells exhibited the typical features of cell death, such as rounding up of the cells and cell shrinkage. Wound-healing assay indicated that GA inhibited the migratory abilities of SW1353 cells. Hoechst 33258 staining assay and Annexin V/PI staining assay exhibited apoptosis induction by GA. To determine the molecular mechanism of GA-induced apoptosis, the expression levels Bcl-2, Bax, caspase-3 and caspase-9 were determined in SW1353 cells treated with GA. We found that GA downregulated the expression of the anti-apoptotic protein Bcl-2, and upregulated the expression of the pro-apoptotic protein Bax, and the activation of caspase-3 and caspase-9. To identify the possible mechanisms, the changes of microRNA expression were tested using the miRCURY™ LNA expression array. It was observed that the miR-518b gene was upregulated in treated cells. Taken together, these data show that GA induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 cells.

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Section: Discussionmentioning

confidence: 99%

Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells

Liang

et al. 2013

International Journal of Oncology

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“…Human immortalized bone marrow stromal cells (iMSC; ABM, Canada) were cultured in minimum essential eagle's media with 10% fetal bovine serum, 1% antibiotics and dexamethasone as 10 -6 M, for seven days with media change every other day [16]. Cells were cultured on two different surfaces, tissue culture plastic and glass, as the latter can modulate cell adhesion that is essential for keratinocyte differentiation [17].…”

Section: Page 4/15mentioning

confidence: 99%

Sprayed cultured autologous keratinocytes in the treatment of severe burns: a retrospective matched cohort study

Karlsson

Steinvall

Olofsson

et al. 2019

Preprint

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Objective: Cultured autologous keratinocytes were used to treat burns since 1981 with many challenges and variable clinical outcome. Our aim was to compare the duration of hospital stay between burned patients received skin grafts with and without cultured autologous keratinocytes, in a retrospective cohort study. Multivariable regression was used to analyse associations between duration of hospital stay and treatment adjusted for age, mortality, size, and depth of the burn. Then, we investigated the differentiation of human stem cell line to keratinocyte-like cells as a future perspective. Results : The median hospital stay in the group given cultured autologous keratinocytes (n=12) was 70 days (10 th – 90 th centile 50-141) and in the matched group 62 days (2 -196) (n=43), p=0.25. The multivariable regression analysis showed a coefficient of 17.36 (95 % CI -17.69 to 52.40), p= 0.32, for hospital stay in the treatment group, compared with the matched group. Our results showed no difference in the duration of hospital stay between the two groups. Therefore, further evaluation for this treatment is needed. Stem cells showed enhanced proliferation and differentiation when cultured on glass surface rather than the classical tissue culture plastic and should be considered for burn management research.

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“…Interestingly, both rat and human osteosarcoma cell lines also show markers of all three germ layers (Russinoff et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…Single cell derived multipotent adult progenitor cells, a subpopulation of mesenchyme stem cells, express a marker of pluripotency, Oct4, as well as genes specific for each of the three germ layers (Ji et al 2008). Interestingly, both rat and human osteosarcoma cell lines also show markers of all three germ layers (Russinoff et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Possible role of H1 histone in replication timing

Flickinger

2014

Develop. Growth Differ.

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AT-rich repetitive DNA sequences become late replicating during cell differentiation. Replication timing is not correlated with LINE density in human cells (Ryba et al. 2010). However, short and properly spaced runs of oligo dA or dT present in nuclear matrix attachment regions (MARs) of the genome are good candidates for elements of AT-rich repetitive late replicating DNA. MAR attachment to the nuclear matrix is negatively regulated by chromatin binding of H1 histone, but this is counteracted by H1 phosphorylation, high mobility group proteins or, indirectly, core histone acetylation. Fewer MAR attachments correlates positively with longer average DNA loop size, longer replicons and an increase of late replicating DNA.

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Osteosarcoma Cells Differentiate into Phenotypes from all Three Dermal Layers

Abstract: Background Osteosarcomas are the most common solid malignant bone tumors, but little is known of their origin.

Cited by 3 publications

References 52 publications

Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells

Gallic acid induces apoptosis and inhibits cell migration by upregulating miR-518b in SW1353 human chondrosarcoma cells

Sprayed cultured autologous keratinocytes in the treatment of severe burns: a retrospective matched cohort study

Possible role of H1 histone in replication timing

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