Osteoprotegerin-eluting nanoparticulate mineralized collagen scaffolds improve skull regeneration

Ren, Xiaoyan; Dejam, Dillon; Oberoi, Michelle K.; Dahan, Natalie J; Zhou, Qi; Huang, Kelly X.; Bedar, Meiwand; Chan, Candace H.; Kolliopoulos, Vasiliki; Dewey, Maynard M.; Harley, Brendan A.C.; Lee, Justine C.

doi:10.1016/j.bioadv.2022.213262

Cited by 4 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osteoprotegerin (OPG) release was measured using a Human OPG DuoSet ELISA (R&D Systems DY805 Lot# P246532, MN, USA) using a previously developed protocol 21,22,29,30 . Seeded scaffolds ( n = 6) were cultured in phenol‐red‐free growth media, with media collected and replaced every 3 days for a total of 28 days.…”

Section: Methodsmentioning

confidence: 99%

“…Osteoprotegerin (OPG) release was measured using a Human OPG DuoSet ELISA (R&D Systems DY805 Lot# P246532, MN, USA) using a previously developed protocol. 21,22,29,30 Seeded scaffolds (n = 6) were cultured in phenol-red-free growth media, with media collected and replaced every 3 days for a total of 28 days. Collected media was pooled into four sample groups, with each group equally representing all included time points: T1 (D3 + D6), T2 (D9 + D12), T3 (D15 + D18 + D21), and T4 (D24 + D27 + D28).…”

Section: Osteoprotegerin Releasementioning

confidence: 99%

“…We have previously described a mineralized collagen–glycosaminoglycan porous scaffold that promotes osteogenic development in human mesenchymal stem cells (hMSCs) in vitro and regenerative CMF healing in vivo without dependence on exogenous factors 15–20 . The porosity of the scaffold design supports rapid cellular infiltration as well as matrix biosynthesis, and it can also be used to induce MSCs within to produce high levels of the glycoprotein osteoprotegerin (OPG), a known inhibitor of osteoclastogenesis, to transiently inhibit osteoclast resorption in order to further improve bone formation 21–23 . While already established as a strong candidate for bone regeneration, we now seek to improve the efficacy of this base material through various means.…”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17][18][19][20] The porosity of the scaffold design supports rapid cellular infiltration as well as matrix biosynthesis, and it can also be used to induce MSCs within to produce high levels of the glycoprotein osteoprotegerin (OPG), a known inhibitor of osteoclastogenesis, to transiently inhibit osteoclast resorption in order to further improve bone formation. [21][22][23] While already established as a strong candidate for bone regeneration, we now seek to improve the efficacy of this base material through various means. We have reported structural modifications that promote conformal fitting of complex defect geometries via the inclusion of macroscale polymeric meshes architectures within the scaffold microstructure [24][25][26] as well as strategies to sequester biomolecules within the mineral phase of the scaffold, further improving cellular bioactivity.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds

Dewey,

Timmer,

Blystone

et al. 2023

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Current treatments for craniomaxillofacial (CMF) defects motivate the design of instructive biomaterials that can promote osteogenic healing of complex bone defects. We report methods to promote in vitro osteogenesis of human mesenchymal stem cells (hMSCs) within a model mineralized collagen scaffold via the incorporation of ascorbic acid (vitamin C), a key factor in collagen biosynthesis and bone mineralization. An addition of 5 w/v% ascorbic acid into the base mineralized collagen scaffold significantly changes key morphology characteristics including porosity, macrostructure, and microstructure. This modification promotes hMSC metabolic activity, ALP activity, and hMSC‐mediated deposition of calcium and phosphorous. Additionally, the incorporation of ascorbic acid influences osteogenic gene expression (BMP‐2, RUNX2, COL1A2) and delays the expression of genes associated with osteoclast activity and bone resorption (OPN, CTSK), though it reduces the secretion of OPG. Together, these findings highlight ascorbic acid as a relevant component for mineralized collagen scaffold design to promote osteogenic differentiation and new bone formation for improved CMF outcomes.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Osteoprotegerin Releasementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds

Dewey,

Timmer,

Blystone

et al. 2023

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our lab has developed an instructive 3D mineralized collagen scaffold that promotes osteogenesis in vitro and in vivo without the need for exogenous factors. We have shown these scaffolds promote osteogenesis in vitro using cells from animals [37][38][39][40] and humans [41][42][43][44][45][46][47][48][49][50][51],…”

Section: Introductionmentioning

confidence: 99%

Donor Variability in Human Mesenchymal Stem Cell Osteogenic Response as a Function of Passage Conditions and Donor Sex

Kolliopoulos,

Tiffany,

Polanek

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Contemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their potential to differentiate to various tissue-specific cells and generate a pro-regenerative secretome. While MSC differentiation and therapeutic potential can differ as a function of matrix environment, it may also be widely influenced as a function of donor-to-donor variability. Further, effects of passage number and donor sex may further convolute the identification of clinically effective MSC-mediated regeneration technologies. We report efforts to adapt a well-defined mineralized collagen scaffold platform to study the influence of MSC proliferation and osteogenic potential as a function of passage number and donor sex. Mineralized collagen scaffolds broadly support MSC osteogenic differentiation and regenerative potency in the absence of traditional osteogenic supplements for a wide range of MSCs (rabbit, rat, porcine, human). We obtained a library of bone marrow and adipose tissue derived stem cells to examine donor-variability of regenerative potency in mineralized collagen scaffolds. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex-based differences. Notably, MSCs from male donors displayed significantly higher metabolic activity and proliferation while MSCs from female donor displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. Our study highlights the essentiality of considering MSC donor sex and culture expansion in future studies of biomaterial regenerative potential.

show abstract

Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds

Kolliopoulos,

Tiffany,

Polanek

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

Contemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their multi‐potent potential and ability to generate a pro‐regenerative secretome. While many have reported the influence of matrix environment on MSC osteogenic response, few have investigated the effects of donor and sex. Here, a well‐defined mineralized collagen scaffold is used to study the influence of passage number and donor‐reported sex on MSC proliferation and osteogenic potential. A library of bone marrow and adipose tissue‐derived stem cells from eight donors to examine donor viability in osteogenic capacity in mineralized collagen scaffolds is obtained. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex‐associated variability in osteogenic capacity. Notably, MSCs from male donors displayed significantly higher cell proliferation while MSCs from female donors displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. The study highlights the essentiality of including donor‐reported sex as an experimental variable and reporting culture expansion in future studies of biomaterial regenerative potential.

show abstract

Osteoprotegerin-eluting nanoparticulate mineralized collagen scaffolds improve skull regeneration

Cited by 4 publications

References 42 publications

Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds

Evaluating osteogenic effects associated with the incorporation of ascorbic acid in mineralized collagen scaffolds

Donor Variability in Human Mesenchymal Stem Cell Osteogenic Response as a Function of Passage Conditions and Donor Sex

Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds

Contact Info

Product

Resources

About