Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles

Zhao, Rui; Shang, Tieliang; Yuan, Bo; Yang, Xiao; Zhu, Xiangdong; Zhang, Xingdong

doi:10.1016/j.bioactmat.2022.01.007

Cited by 23 publications

(22 citation statements)

References 63 publications

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“…The orthopedic application of CaP bioceramics in bone repair was carried out in the approximately 1980s. , Previous studies have confirmed that CaP bioceramics can be used in fresh tooth sockets for bone regeneration and spinal fusion and as a bone void filler in a variety of bone fractures. ,, It is well-accepted that the porous structure is crucial for osteoinductive performance of bioceramics and subsequent bone regeneration. ,, Our group was the first few research groups who introduced the H 2 O 2 foaming method to prepare porous CaP bioceramics in 1998 and confirmed that this strategy can induce the formation of abundant interconnected open pores with a variety of sizes. , This porous structure later was proven to be beneficial in blood vessel formation and excellent osteogenic capacity. ,, In 2019, we demonstrated that porous CaP bioceramics prepared by the gas-forming method can be clinically used during children’s endoscopic neurosurgery so as to prevent postoperative cerebrospinal fluid leakage in endoscopic neurosurgery of young patients . Although the preparation and use of multiphase CaP bioceramics have been extensively reported previously, as far as we know, no study has designed a porous CaP bioceramic with a spherical core–shell structure for bone repair.…”

Section: Discussionmentioning

confidence: 99%

“…Next, we assessed the bone forming ability of BCP@HA, HA@BCP, HA, and BCP bioceramic granules in a rat model of femur defects established previously. , In the current study, the results demonstrated that the stride length results confirmed that the BCP@HA group had significantly better stride recovery than the HA and BCP groups; however, there was no difference was found in the stride length between the BCP@HA and HA@BCP groups. In addition, blood samples were collected from SD rats at weeks 8 and 12 after implantation.…”

Section: Discussionmentioning

confidence: 99%

“…The femurs were imaged under scanning conditions similar to the material characterization. During the reconstruction, a global threshold was used to differentiate between the material and bone. ,, The ratio of bone volume to tissue volume (BV/TV) represents the extent of new bone formation within the defect, based on previous studies . The 1 × 2 × 2 mm 3 region around the defect was taken as VOI to investigate the level of the adjacent bone loss when implanted with different bioceramic granules.…”

Section: Methodsmentioning

confidence: 99%

“…Degradation Profile of the Bioceramic Granules. According to our previously reported method, 48 the degradation rate of the samples was quantified by measuring the concentrations of Ca 2+ and PO 4 3− free ions in a standard Tris-buffer solution. Briefly, the BCP@ HA, HA@BCP, HA, and BCP bioceramics were soaked in Tris−HCl buffer (pH = 7.6) with a fixed ratio of 1 g:100 mL of bioceramics to buffer and kept at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The assessment of spontaneous behavior was performed 7 weeks after surgery, and the method was based on our previous study. 48 Briefly, all animals will be trained to run continuously on a track consisting of plain paper and solid walls prior to testing. Then, the hind paws of the rats were stained with red ink and allowed to run freely.…”

Section: Quantitative Reverse-transcription Polymerase Chain Reactionmentioning

confidence: 99%

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Core–Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration

Long

Chen

et al. 2022

ACS Appl. Mater. Interfaces

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Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis. Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability. Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core−shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell). The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H 2 O 2 foaming process. These granules could be stacked to build a porous structure with a porosity of 65−70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth. In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts. In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups. From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone. A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction. The current study demonstrated that the core−shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Quantitative Reverse-transcription Polymerase Chain Reactionmentioning

confidence: 99%

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Core–Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration

Long

Chen

et al. 2022

ACS Appl. Mater. Interfaces

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Engineered Cell‐Laden Armor Unit‐Mimicking Bioceramic Granules for Bone Regeneration

Wu,

Xing,

Zhao

et al. 2023

Adv Funct Materials

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Bioceramic granules have been used as fillers for the repair of various bone defects, but structural instability leads to susceptibility to displacement or dislodgement in practice. Armor unit is a concrete block placed along the breakwater to resist waves and protect the dike. Inspired by this, an armor unit‐mimicking (AU) bioceramic granule by three‐dimentional (3D) printing is developed, and combine it with adipose‐derived stem cells (ADSCs) overexpressing fibroblast growth factor 23 (FGF23) to construct tissue‐engineered bone. In vitro results show that AU granules and FGF23 transfection synergistically promote osteogenic differentiation of ADSCs. For the repair of beagle femoral metaphyseal defects, the implanted AU granules are stacked and interlocked to form an impact‐resistant porous scaffold. With the assistance of ADSCsFGF23, AU granules exhibit enhanced new bone formation and host bone maintenance. Taken together, these findings demonstrate the potential of 3D‐printed AU granules in combination with FGF23‐overexpressing ADSCs as a novel bone void filler for ease of handling and enhanced bone regeneration.

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Advancing Osteoporotic Bone Regeneration Through Tailored Tea Polyphenols Functionalized Micro‐/Nano‐ Hydroxyapatite Bioceramics

Zhao,

Qian,

Zhu

et al. 2024

Adv Funct Materials

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Efforts to develop advanced bone substitutes for effective bone regeneration in substantial defects have led to the fabrication of tissue‐engineered scaffolds. These scaffolds, featuring hierarchical structures, specific chemical compositions, and functional qualities, are essential in mimicking native bone tissue. Inspired by the biomineralization process, hydrothermal treatment is used to synthesize micro‐/nano‐hydroxyapatite bioceramics functionalized with tea polyphenols (TP‐nwHA), closely resembling the structure of bone‐like apatite induced by hydroxyapatite bioceramics in vivo. The in vitro results demonstrate TP‐nwHA's superior biocompatibility, enhancing cell proliferation and adhesion. Furthermore, TP‐nwHA scaffolds significantly influence mesenchymal stem cells, promoting osteogenic differentiation while inhibiting osteoclastogenic differentiation. The upregulation of osteogenic proteins BMP2 and ITGB1, along with the downregulation of osteoclastic proteins FGF21 and IGFBP1, demonstrate the synergistic effect of the biomimetic structure and polyphenols on the activation of the MAPK signaling pathway. In vivo, TP‐nwHA showe early angiogenic capabilities, leading to improved bone regeneration in critical‐size femoral bone defects in osteoporotic rats. Histological staining confirms the complete bridging of defects with new bone tissue in the TP‐nwHA group, and nanoindentation tests indicate the formation of mature mineralized bone tissue. Collectively, these findings suggest a novel strategy for fabricating bone‐mimicking constructs with potential applications in disease modeling.

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Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles

Cited by 23 publications

References 63 publications

Core–Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration

Core–Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration

Engineered Cell‐Laden Armor Unit‐Mimicking Bioceramic Granules for Bone Regeneration

Advancing Osteoporotic Bone Regeneration Through Tailored Tea Polyphenols Functionalized Micro‐/Nano‐ Hydroxyapatite Bioceramics

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