Osteopontin Promotes Invasion, Migration and Epithelial-Mesenchymal Transition of Human Endometrial Carcinoma Cell HEC-1A Through AKT and ERK1/2 Signaling

Abstract

Background/Aims: Osteopontin (OPN) is an Extracellular Matrix (ECM) molecule and is involved in many physiologic and pathologic processes, including cell adhesion, angiogenesis and tumor metastasis. OPN is a well-known multifunctional factor involved in various aspects of cancer progression, including endometrial cancer. In this study, we examined the significance of OPN in endometrial cancer. Methods: The proliferation, migration and invasion ability of HEC-1A cells were detected by Cell Counting Kit-8 (CCK-8), Wound scratch assay and transwell. Western blots were employed to detect the expression of Matrix metalloproteinase-2 (MMP-2) and epithelial-mesenchymal transition (EMT)-related factors in HEC-1A cells treated with rhOPN. Results: rhOPN promotes cell proliferation, migration and invasion in HEC-1A cells. rhOPN influenced EMT-related factors and MMP-2 expression in HEC-1A cells. rhOPN promoted HEC-1A cells migration, invasion and EMT through protein kinase B (PKB/AKT) and Extracellular regulated protein kinases (ERK1/2) signaling pathway. Conclusions: These results may open up a novel therapeutic strategy for endometrial cancer: namely, rhOPN have important roles in controlling growth of endometrial of cancer cells and suggest a novel target pathway for treatment of this cancer.

Osteopontin Promotes Cell Migration and Invasion, and Inhibits Apoptosis and Autophagy in Colorectal Cancer by activating the p38 MAPK Signaling Pathway

“…Furthermore, the secretory OPN levels of 2 metastatic cell lines, SW620 and HCT116, and 2 non-metastatic cell lines, DLD1 and SW480, were investigated, the OPN level of the SW620 and HCT116 cell lines were found to approximate a concentration of 300 ng per ml [8]. A recent study of uterine endometrial carcinoma showed that exogenous OPN was capable of promoting tumor cells invasion, migration, and epithelial-mesenchymal transition (EMT) [27]. To evaluate the possible roles of extracellular OPN, our team used OPN-specific antibody to block the secreted OPN by HCT116 cells for the purpose of analyzing the influence of autocrine OPN.…”

Up-Regulated Expression of Matrix Metalloproteinases in Endothelial Cells Mediates Platelet Microvesicle-Induced Angiogenesis

“…MMPs promote endothelial cell migration and tube formation by proteolytically remodelling the basement membrane [2, 38]. Among these, MMP-2 and MMP-9 have been emphasized because their type IV collagenase activity is essential during the initial phase of angiogenesis [20, 39].…”

Capn4 Enhances Osteopontin Expression through Activation of the Wnt/β-Catenin Pathway to Promote Epithelial Ovarian Carcinoma Metastasis

“…OPN is a secreted multifunctional glycophosphoprotein that is overexpressed in various tumors and plays important roles in cell adhesion and migration [21-23]. Several experiments have demonstrated that OPN plays a crucial role in ovarian cancer.…”