Osteopontin mRNA expression by rat mesothelial cells exposed to multi-walled carbon nanotubes as a potential biomarker of chronic neoplastic transformation in vitro

Sridharan, Sreepradha; Taylor-Just, Alexia J.; Bonner, James C.

doi:10.1016/j.tiv.2021.105126

Cited by 3 publications

(5 citation statements)

References 41 publications

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“…Anchorage-independent growth (soft-agar colony formation assay) and migration and invasion assays were considered basic carcinogenic hallmark assays for almost all instances of nm-induced cell transformation when a human cell line was targeted. As exceptions, few studies chose only proliferation, migration, and invasion assays as proof of induced cell transformation [65,70,72]. Matrix metalloproteinases (MMPs), specifically MMP-2 and MMP-9, were used as biomarkers for nmNM-induced neoplastic transformation [37,39,42,43,60,72].…”

Section: Carcinogenic-hallmark-related Assaysmentioning

confidence: 99%

“…As exceptions, few studies chose only proliferation, migration, and invasion assays as proof of induced cell transformation [65,70,72]. Matrix metalloproteinases (MMPs), specifically MMP-2 and MMP-9, were used as biomarkers for nmNM-induced neoplastic transformation [37,39,42,43,60,72]. Several studies also performed wound-healing assays, angiogenesis, and xenograft tumorigenesis (in nude mice) (Table 1) as evidence of nm-induced in vitro transformation.…”

Section: Carcinogenic-hallmark-related Assaysmentioning

confidence: 99%

“…In general, the molecular mechanism of cell transformation, distinct from asbestos, shared similarities among CNTs (MWCNT vs. SWCNT). The studies with various carbon-based nanomaterials exhibited similar responses to malignant transformation hallmarks, such as migration and invasion [72], gene expression signatures [71], and cancer stem cell-like properties [67]. Size-related studies (diameter or aspect ratio) in CNTs did not obtain a clear outcome because the results variation is possibly related to the cell line of choice.…”

Section: The Influence Of Physicochemical Properties Of Nms In Cell T...mentioning

confidence: 99%

“…Global gene expression analysis of the same model delineated activation of the pAkt/p53/Bcl-2 signaling axis, Ras family proteins for cell-cycle control, Dsh-mediated Notch 1, and the downregulation of apoptotic genes BAX and Noxa [87]. Other global gene expression studies evidenced that CNTinduced neoplastic-like transformation in normal mesothelial cells (MeT5A) was associated with overexpression of cortactin and H-Ras-ERK1/2 signaling (in the case of SWCNT exposure) [73], activating the MMP-2 gene and its critical role in an invasive phenotypic trait (MWCNT and SWCNT exposures) [72]. Whole-genome microarray further demonstrated differential signaling pathways between CNT-induced vs. asbestos-induced malignant transformation in primary small airway epithelial cells (SAECs).…”

Section: Other Mechanisms Of Cta-induced Nmmentioning

confidence: 99%

See 3 more Smart Citations

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

Chatterjee

Alfaro-Moreno

2023

IJMS

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This review explores the application of in vitro cell transformation assays (CTAs) as a screening platform to assess the carcinogenic potential of nanomaterials (NMs) resulting from continuously growing industrial production and use. The widespread application of NMs in various fields has raised concerns about their potential adverse effects, necessitating safety evaluations, particularly in long-term continuous exposure scenarios. CTAs present a realistic screening platform for known and emerging NMs by examining their resemblance to the hallmark of malignancy, including high proliferation rates, loss of contact inhibition, the gain of anchorage-independent growth, cellular invasion, dysregulation of the cell cycle, apoptosis resistance, and ability to form tumors in experimental animals. Through the deliberate transformation of cells via chronic NM exposure, researchers can investigate the tumorigenic properties of NMs and the underlying mechanisms of cancer development. This article examines NM-induced cell transformation studies, focusing on identifying existing knowledge gaps. Specifically, it explores the physicochemical properties of NMs, experimental models, assays, dose and time requirements for cell transformation, and the underlying mechanisms of malignancy. Our review aims to advance understanding in this field and identify areas for further investigation.

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Section: Carcinogenic-hallmark-related Assaysmentioning

confidence: 99%

Section: Carcinogenic-hallmark-related Assaysmentioning

confidence: 99%

Section: The Influence Of Physicochemical Properties Of Nms In Cell T...mentioning

confidence: 99%

Section: Other Mechanisms Of Cta-induced Nmmentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

Chatterjee

Alfaro-Moreno

2023

IJMS

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“…Because of their extremely small particle size, CNTs may enter the body through the pulmonary blood barrier or the skin via simple diffusion or penetration [ 12 ]. Sridharan demonstrated that MWCNTs are carcinogenic in vitro [ 13 ]. The above experiments prove that CNTs can easily pass through cell membranes by simple osmosis.…”

Section: Introductionmentioning

confidence: 99%

Dibutyl Phthalate Adsorbed on Multiwalled Carbon Nanotubes Causes Fetal Developmental Toxicity in Balb/C Mice

Qin

Peng

et al. 2023

Toxics

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This study investigated whether using multiwalled carbon nanotubes (MWCNTs) as a carrier for dibutyl phthalate (DBP) could delay the degradation rate of DBP in mice and increase its estrogen-like interference effect. Pregnant Balb/C mice were divided into four groups and exposed to different treatments via tail-vein injection every 3 days until gestational day 20. The female and male mice were then sacrificed for toxicological study. The results showed that the combination of MWCNTs and DBP resulted in a higher fetal mortality rate than if the mice were exposed to MWCNTs or DBP alone. H&E staining showed that the estrous period of the exposed mice was delayed, the development of oocytes was blocked in the combination group, the number of spermatogenic cells decreased, and the quality of sperm decreased. Our experiment showed that the expression levels of the genes involved in sex hormone synthesis in the testis and ovaries were significantly increased after combined treatment compared with the MWCNT group (p < 0.01). The study suggests that DBP degradation is delayed when absorbed on MWCNTs, which increases its estrogen-like interference and interferes with fetal development, ultimately leading to increased fetal mortality.

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Unlocking the potential of nanocarrier-mediated mRNA delivery across diverse biomedical frontiers: A comprehensive review

Pawar,

Pingale,

Garkal

et al. 2024

International Journal of Biological Macromolecules

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Osteopontin mRNA expression by rat mesothelial cells exposed to multi-walled carbon nanotubes as a potential biomarker of chronic neoplastic transformation in vitro

Cited by 3 publications

References 41 publications

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

In Vitro Cell Transformation Assays: A Valuable Approach for Carcinogenic Potentiality Assessment of Nanomaterials

Dibutyl Phthalate Adsorbed on Multiwalled Carbon Nanotubes Causes Fetal Developmental Toxicity in Balb/C Mice

Unlocking the potential of nanocarrier-mediated mRNA delivery across diverse biomedical frontiers: A comprehensive review

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