Osteopontin in gingival crevicular fluid

Abstract

Osteopontin (OPN) is a major glycosylated phosphoprotein in bone matrix and is produced by several cells including osteoblasts, osteoclasts and macrophages. OPN levels increase in active sites of bone metabolism. Recently, several bone-related proteins were identified in gingival crevicular fluid (GCF) to seek markers of alveolar bone resorption in periodontal disease. In this study, we investigated the existence of OPN in GCF and the correlation between OPN level in GCF and probing depth (PD) of sampling sites in 98 periodontitis patients and 35 healthy subjects. An immunoblotting analysis using 10% polyacrylamide gel showed that two forms of OPN with molecular masses of 54 and 66 kDa and several degraded fragments were detected in most GCF samples from diseased sites (PD > 4 mm). In GCF samples from healthy sites (PD < or = 3 mm), only one form (54 kDa) was observed, but any degraded fragments were not detected. When OPN amounts in GCF samples were determined by ELISA, a weak. but significant correlation was observed between OPN amount in GCF and PD (r=0.32, p=0.0013). These results demonstrate that OPN exists in GCF and that OPN level in GCF increases with the progression of periodontal disease.

Comparison Of Bone Turnover Markers In Gingival Crevicular Fluid Of Elderly Individuals With Different Periodontal Diseases

“…25 Kido et al reported that OPN in GCF may be considered as a marker of alveolar bone loss in periodontitis, while Sharma and Pradeep reported that GCF OPN levels were higher in periodontitis patients compared to those with gingivitis and healthy subjects. 24,25 Conversely, the present study showed similar total amounts of OPN GCF levels in all geriatric groups. Neighboring tissues including alveolar bone and cementum, macrophages in periodontal tissues, blood and salivary glands function as the source of OPN in GCF.…”

“…OPN, is mainly involved in enhancing osteoclastic action and has also been detected in GCF in some aforementioned studies. 24,25 The effects of initial periodontal therapy also confirmed that OPN levels significantly decreased and were correlated with decreasing clinical periodontal parameters. 25 Kido et al reported that OPN in GCF may be considered as a marker of alveolar bone loss in periodontitis, while Sharma and Pradeep reported that GCF OPN levels were higher in periodontitis patients compared to those with gingivitis and healthy subjects.…”

“…Neighboring tissues including alveolar bone and cementum, macrophages in periodontal tissues, blood and salivary glands function as the source of OPN in GCF. 24 Moreover, the reduced inflammatory response may also play a role of similar pattern of OPN in GCF. 3 It appears that GCF OPN levels are not related to inflammatory changes in elderly individuals with periodontal disease, so this could partly explain the similar results observed in all groups.…”

Gingival Crevicular Fluid Calprotectin, Osteocalcin and Cross-Linked N-Terminal Telopeptid Levels in Health and Different Periodontal Diseases

“…The increased recognition of the interactions between the cells of the immune and skeletal system has led to the seeking of a variety of regulatory molecules that play a role in the progression of periodontal disease [25][26][27]. In previous studies, the bestknown markers of the bone turnover have been widely investigated in GCF samples of patients with different periodontal diseases and implicated to be associated with the progression of periodontal diseases [28][29][30]. Several investigations provided contradictory evidence about the role of osteocalcin in GCF of patients with periodontitis [31][32][33].…”

Recent Advances in Salivary Proteomics, Genomics and Transcriptomics: A Reliable Tool in Periodontal diagnosis – A Review

“…Several investigations on OPN indicated that OPN concentrations in gingival crevicular fluid increased proportionally with the progression of disease; and OPN levels in GCF were significantly reduced following nonsurgical periodontal treatment (33,34).…”