Osteopontin improves sperm capacitation and in vitro fertilization efficiency in buffalo (Bubalus bubalis)

Boccia, Lorenzo; Francesco, S. Di; Neglia, Gianluca; Blasi, M. De; Longobardi, V.; Campanile, Giuseppe; Gasparrini, B.

doi:10.1016/j.theriogenology.2013.04.017

Cited by 32 publications

(44 citation statements)

References 35 publications

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“…In the present study, OPN level was measured quantitatively by ELISA method which is simple, rapid and cheaper that could be performed routinely in laboratory to know fertility score of bulls. In vitro studies also confirmed that OPN supplementation improved the capacitation, in vitro fertilization, cleavage rate and embryonic development (Boccia et al., ; Erikson et al., ; Goncalves et al., ; Hao et al., ; Monaco et al., ). Actually, OPN is secreted by seminal vesicles in bovines and binds to sperm at the time of ejaculation and remain bound until sperm reach the oviduct (Souza, Moura, Monaco, & Killian, ).…”

Section: Discussionmentioning

confidence: 83%

“…() reported the presence of four fertility‐associated proteins in the seminal plasma of bulls, one of them later identified as OPN (Cancel et al., ). In in vitro studies, OPN treatment influenced sperm capacitation, in vitro fertilization, cleavage rate and embryo development (Boccia et al., ; Erikson, Way, Chapman, & Killian, ; Goncalves, Chapmana, Bertolla, Eder, & Killian, ; Hao et al., ; Monaco et al., ). But the exact endogenous level of OPN in seminal plasma has not been reported in bulls in relation to fertility till now.…”

Section: Introductionmentioning

confidence: 99%

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Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Kumar

Saini

Kumar

et al. 2016

Reprod Domestic Animals

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This study was attempted to identify subfertile bulls by quantifying the endogenous levels of osteopontin (OPN), total antioxidant capacity (TAC) and malondialdehyde (MDA) in seminal plasma of buffalo bulls. On the basis of conception rate, buffalo bulls were classified into two groups: high-fertile (conception rate >50%) and subfertile bulls (conception rate <40%). A total of 100 ejaculates (10 ejaculates from each bull) were collected through artificial vagina method. The concentration of OPN, TAC and catalase (CAT) of high-fertile bulls was found to be higher (p < .05) than that of subfertile bulls. Further, MDA level in seminal plasma was found to be lower (p < .05) in high-fertile bulls compared with subfertile bulls. The fertility status had no effect on the superoxide dismutase (SOD) concentration in seminal plasma of both the groups. The levels of OPN (r = .678, p = 0.013) and TAC (r = .648, p = .042) were found to be positively correlated with bull fertility and the level of MDA (r = -.718, p = .019) was found to be negatively correlated with bull fertility. However, the fertility of bulls was not found to be significantly correlated with SOD, CAT and sperm motility. In conclusion, seminal OPN, TAC and MDA tended to be more realistic in identification of subfertile bulls from breeding herds.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Kumar

Saini

Kumar

et al. 2016

Reprod Domestic Animals

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“…In particular, in our system, an increased capacitation is recorded either after 2 h or 4 h according to the capacitating agents employed (Boccia et al. ; Elkhawagah et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate the sperm viability and the acrosome reaction, the sperm were fixed and stained with Trypan blue–Giemsa (Boccia et al. ). Sperm were counted under differential interference light microscopy (Nikon Diaphot 300), and the number of acrosome‐intact live (AILS), acrosome‐intact dead (AIDS), acrosome‐reacted live (ARLS) and acrosome‐reacted dead (ARDS) sperm was recorded.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Relaxin on Fertility Parameters of Frozen–Thawed Buffalo (Bubalus bubalis) Sperm

Elkhawagah

Longobardi

Neglia

et al. 2015

Reprod Domestic Animals

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The aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. Sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. Sperm viability, motility, capacitation and the effect of relaxin on the fertilizing ability after heterologous IVF were evaluated. Although viability was not affected, relaxin increased (p < 0.05) sperm motility compared to the negative and positive controls both after 2 h (60.0 ± 2.0, 60.0 ± 3.1, 68.3 ± 1.7 and 69.4 ± 2.7, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) and 4 h (55.0 ± 2.5, 53.3 ± 3.0, 62.2 ± 3.0 and 65.0 ± 3.2, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin) incubation. When sperm were incubated with both 100 ng/ml relaxin and heparin, a decrease (p < 0.01) of pattern A, that is low capacitation level, was observed compared to the negative control both after 2 h (54.4, 34.3 and 36.4%, respectively, in negative control, positive control and 100 ng/ml relaxin) and 4 h (51.9, 35.0 and 34.3%, respectively, in negative control, positive control and 100 ng/ml relaxin). Moreover, an increase (p < 0.01) of pattern EA, that is high capacitation level, was recorded with 100 ng/ml relaxin and heparin compared to the negative control both after 2 h (44.1, 59.3 and 57.7%, respectively, in negative control, positive control and 100 ng/ml relaxin) and after 4 h (43.0, 54.4 and 56.0%, respectively, in negative control, positive control and 100 ng/ml relaxin). Finally, relaxin increased (p < 0.01) cleavage rate compared to the negative control (57.1 ± 4.4, 72.5 ± 6.0, 71.4 ± 5.5 and 73.6 ± 2.9, respectively, in negative control, positive control, 50 and 100 ng/ml relaxin). In conclusion, relaxin has a beneficial effect on motility, capacitation and fertilizing ability of frozen-thawed buffalo sperm.

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Advances in Embryo Production in Buffaloes: In Vivo Versus In Vitro Procedures

Ohashi

Kumar

Filho

et al. 2022

Biotechnological Applications in Buffalo Research

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Osteopontin improves sperm capacitation and in vitro fertilization efficiency in buffalo (Bubalus bubalis)

Cited by 32 publications

References 35 publications

Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Effect of Relaxin on Fertility Parameters of Frozen–Thawed Buffalo (Bubalus bubalis) Sperm

Advances in Embryo Production in Buffaloes: In Vivo Versus In Vitro Procedures

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