Osteopontin Expression in Clonal Dental Pulp Cells

Oida

et al. 2010

The purpose of the current research is to elucidate of gene expression of the tooth germ. Fresh permanent incisor and molar teeth germs were extracted from mandibles of 6-month-old pig. Extracted human premolars for orthodontic reasons were used as sample of the completed tooth root. Apical and central samples were prepared from pulp tissue. mRNA expression by RT-PCR and protein expression of BMP in the pulp tissue were detected. DMP1, BMP2 expressions were similar in all samples. OP expression was weaker in centrum of the molar tooth as compared to the other parts. OC was not expressed. BMP4 expression was strong at the incisor apical part, though it was not expressed at the molar central area. BMP5 was weakly expressed at the incisor central and molar apical areas. Though BMP7 expression was remarkably detected at the incisor and molar apexes, its expression was almost absent at the central area. Moreover for examining the specific expression of BMP7 at the apex, central superficial layer corresponding to the neck of the incisor and molar teeth was prepared. The result was that BMP7 was strongly expressed at the apex than in the central superficial layer and only a little expression was detected at central superficial layer of the incisor and molar tooth. In the current research, expressions of OP, DMP, BMP 2, 4, 5, 7 were seen in the tooth germ tissue. Thus the results suggested that expression of these genes played a central role in differentiation of the pulp. BMP7 was also suggested to have a specific role in apex formation (differentiation). .

Section: Discussionmentioning

confidence: 99%

“…The main matrix component forming the pulp is generally same for mesenchymal tissue, and collagen, proteoglycan as a noncollagenous protein 7) , osteopontin 8,9) , osteocalcin 10) , dentine matrix protein1 11,12) etc. have been known to be produced, all of these materials are also known to be included in bone, dentine and cementum.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression on Developmental Process of Porcine Pulp Cell

Oida

et al. 2010

“…However, compared to the researches done on the crown of the tooth such as in the enamel and dentin, studies in the formation of periodontal tissues are very few. The organic component of the furcation and periodontal tissues is in general similar to connective tissues which are composed of collagenous and non-collagenous proteins like proteoglycans 10) A osteopontin 11,12) , osteocalcin 13) , dentin matrix protein- 1 14-15) . Those proteins are also included in the formation of pulp and dentin.…”

Section: Introductionmentioning

confidence: 99%

Gene Expression During the Formation of Furcation in Porcine Tooth Germ

Kanazashi

et al. 2012

In recent years, many things have progressed and became clear in the study of genes involved in tooth development. However, the genes associated in the formation of periodontal tissues particular those studies involved in the formation of furcation are few. We have carried out a research to examine the gene expression of cementoblasts in the furcation area in porcine tooth germ. Porcine tooth germs were used as samples excised during the formation of mandibular molars and the cementoblasts around the root and at the furcation were prepared. Total RNA was isolated from the samples followed by PCR amplifying specific genes using PCR templates. Further, significant difference with respect to gene expression was determined by semi-quantitative PCR from the electrophoresis image. Also, after the removal of tooth germs, tissues were processed in routine tissue preparation, embedded in paraffin, sectioned into 5 um thickness, stained with H and E and examined under the light microscope.Amelogenin, enamelin, ameloblastin, MMP-20, Klk-4 expressions were observed uniformly by the cementoblasts at the furcation of the tooth but only amelogenin and ameloblastin were observed by the cementoblasts around the root of the tooth. Also, a typical strong Msx2 homeobox gene by the cementoblasts at the furcation was observed with an expression level of approximately 64 times than the cementoblasts around the root.The present study clearly showed that specific genes are involved in the formation of furcation. A strong Msx2 gene expression was observed in the tooth germ and even at the furcation suggesting that Msx2 plays an important role in the process of periodontal tissue formation and maturation of furcation.

“…The major component of the matrix that makes up the pulp is generally a connective tissue composed of collagen and proteoglycan and non-collagenous protein 2) such as osteopontin 3,4) , osteocalcin 5) , DMP1 6,7) are produced.…”

Section: Introductionmentioning

confidence: 99%

Effects of Carbon Dioxide Laser Irradiation on Human Pulp Fibroblasts

Kobayashi

et al. 2012

The aim of this study was to evaluate the effects of pulsed CO 2 laser irradiation on human pulp fibroblast.Pulp fibroblasts were collected from extracted human third molars. The fibroblasts were kept in humidified atmosphere at 37 o C with 5% CO 2 . Experiments were performed with cells not exceeding 7 passages. Pulp fibroblast cells were irradiated with CO 2 laser device (BEL-LUXER super pulse laser). Laser irradiation was applied continuously with a wave of E16 mode for 90 sec. The specimens were cultured (10-day) with or without CO 2 laser irradiation. Laser-irradiated medium was separated from cell culture dish and the protein, calcium and phosphate were quantified from the supernatants. After cultivation, fibroblast count was determined using a hemocytometer (Bright-line Hausser Scientific Horsham, PA USA). Total RNA was extracted from fibroblast cells and RT-PCR analysis was performed. The protein concentration increased in samples treated with CO 2 laser irradiation. The number of fibroblast cells in laser irradiated group was significantly higher than the untreated group (n=8, t-test, p<0.01). HSP27 and 60 mRNA expressions were detected in both untreated and laser irradiated groups. However, HSP 70 was found only in laser irradiation group. OC was presented in both untreated and laser irradiated groups. Other markers of calcification were not detected in all specimens.It was suggested that pulsed CO 2 laser irradiation tends to accelerate cell proliferation and induces HSP70 expression.