Osteopontin-derived synthetic peptide SVVYGLR upregulates functional regeneration of oral and maxillofacial soft-tissue injury

Tanaka, Susumu; Hamada, Y.; Yokoyama, Yuhki; Yamamoto, Hirofumi; Kogo, Mikihiko

doi:10.1016/j.jdsr.2021.09.002

Cited by 7 publications

(2 citation statements)

References 70 publications

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“…The possible biological role of this peptide sequence has attracted attention since it becomes exposed following thrombin cleavage of osteopontin. 2,37 Synthetic SVVYGLR peptide has been shown to stimulate proliferation and migration of endothelial cells 38 as well as to stimulate the functional regeneration of oral skeletal muscle. 39 The effect of the SVVYGLR peptide has been shown to be mediated through binding to α4 and α9 integrins.…”

Section: Discussionmentioning

confidence: 99%

Identification of an osteopontin-derived peptide that binds neuropilin-1 and activates vascular cells

Chen,

Gialeli,

Shen

et al. 2023

Preprint

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Background: The osteopontin-derived peptide FOL-005 has been shown to stimulate hair growth. Using ligand-receptor glycocapture technology we identified neuropilin-1 (NRP-1), a known co-receptor for vascular endothelial growth factor (VEGF) receptors, as the most probable receptor for FOL-005. Considering that induction of perifollicular angiogenesis by VEGF is a critical step in activation of the anagen growth phase of hair follicles, we investigated the effect of the more stable FOL-005 analogue FOL-026 on vascular cells. Methods: X-ray diffraction and microscale thermophoresis analysis were used to determine receptor binding of FOL-peptides. Cultured human arterial smooth muscle cells were used for studies of cell proliferation, migration, intracellular signaling, and apoptosis. A Matrigel plug assay was used to study angiogenesis in vivo. RNA seq. was used to analyze changes in gene expression patterns. Results: FOL-026 was found to share binding site with VEGF in the NRP-1 b1-subdomain. Stimulation of human umbilical vein endothelial cells with FOL-026 resulted in phosphorylation of AKT and ERK1/2, increased cell growth and migration, stimulation of endothelial tube formation and inhibition of apoptosis in vitro, as well as activation of angiogenesis in vivo. Down-regulation of NRP-1 by NRP-1-specific small interfering RNA blocked the stimulatory effects of FOL-026 on endothelial cells. RNA sequencing showed that FOL-026 activated pathways involved in tissue repair. Exposure of human coronary artery smooth muscle cells to FOL-026 stimulated cell proliferation, migration, inhibited apoptosis, and induced VEGF gene expression by an NRP-1-dependent mechanism. Conclusions: These findings identify NRP-1 as the receptor for FOL-026 and show that its biological effects mimic that of growth factors binding to the VEGF receptor family. They also suggest that FOL-026 may have therapeutical potential in conditions that require vascular repair and / or enhanced angiogenesis.

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Section: Discussionmentioning

confidence: 99%

Identification of an osteopontin-derived peptide that binds neuropilin-1 and activates vascular cells

Chen,

Gialeli,

Shen

et al. 2023

Preprint

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“…Severe oral and maxillofacial skin injury caused by trauma or surgery leads to undesirable healing, such as delayed wound closure and scar formation. This results in physical dysfunction and affects maxillofacial appearance ( Tanaka et al, 2021 ). Wound healing is a complex process involving fibroblast and myofibroblast subpopulations, growth factors, cytokines, and extracellular matrix (ECM) components ( Chang et al, 2002 ; Darby and Hewitson, 2007 ; Driskell et al, 2013 ; Dalisson and Barralet, 2019 ; Hinz and Lagares, 2020 ; Kim et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Engineered stem cell exosomes for oral and maxillofacial wound healing

Hao

Duan²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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Wound healing of the oral and maxillofacial area affects the quality of life and mental health of the patient; therefore, effective therapies are required to promote wound healing. However, traditional treatment methods have limited efficacy. Exosomes secreted by stem cells used for oral and maxillofacial wound healing have shown outstanding results. Stem cell-derived exosomes possess the regenerative and repair ability of stem cells. Moreover, they are nontumorigenic and have good biosafety. However, the application of natural stem cell exosomes is limited owing to their low yield, impurity, lack of targeting, and low drug delivery rate. Many modification methods have been developed to engineered stem cell exosomes with beneficial properties, such as modifying parent cells and directly processing stem cell exosomes. These methods include coincubation, genetic engineering, electroporation, ultrasound, and artificial synthesis of engineered stem cell exosomes. These engineered stem cell exosomes can cargo nucleic acids, proteins, and small molecules. This gives them anti-inflammatory and cell proliferation regulatory abilities and enables the targeted promotion of efficient soft tissue repair after trauma. Engineered stem cell exosomes can decrease inflammation, promote fibroblast proliferation, and angiogenesis, and decrease scar formation to promote oral and maxillofacial wound healing, including diabetic and burn wounds. Thus, engineered stem cell exosomes are an effective treatment that has the potential for oral and maxillofacial wound healing.

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3D-printed bone regeneration scaffolds modulate bone metabolic homeostasis through vascularization for osteoporotic bone defects

Yan,

Zhang,

Qin

et al. 2024

Biomaterials

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Osteopontin-derived synthetic peptide SVVYGLR upregulates functional regeneration of oral and maxillofacial soft-tissue injury

Cited by 7 publications

References 70 publications

Identification of an osteopontin-derived peptide that binds neuropilin-1 and activates vascular cells

Identification of an osteopontin-derived peptide that binds neuropilin-1 and activates vascular cells

Engineered stem cell exosomes for oral and maxillofacial wound healing

3D-printed bone regeneration scaffolds modulate bone metabolic homeostasis through vascularization for osteoporotic bone defects

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